Home / Protease Substrates / Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

Catalog #: ES005
18 Citations 1 Review Datasheet
Substrate for ECE-1, ACE, Cathepsin A, Cathepsin X/Z/P, Neprilysin, and Insulysin
Catalog # Availability Size / Price Qty
ES005
R&D Systems Fluorogenic Peptide Substrates
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Product Details
Citations (18)
FAQs
Supplemental Products
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Related Research Areas

Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate Summary

Product Specifications

Purity
>95%, by HPLC.
Activity
The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence. It is an excellent substrate for endothelin-converting enzyme-1 (ECE-1) and neprilysin. The cleavage site by ECE-1 is the peptide bond between Ala and Phe (Johnson, G.D. and Ahn, K., 2000, Anal. Biochem. 286:112). The substrate is derived from bradykinin. It is also an excellent substrate for ACE and active Cathepsin A and X/Z/P.
Source

Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH
Mca: (7-Methoxycoumarin-4-yl)acetyl, Dnp: 2, 4-Dinitrophenyl.
Predicted Molecular Mass
1388 Da

Product Datasheets

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ES005

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ES005

Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light

Citations for Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Obese Male Mice Exposed to Early Life Stress Display Sympathetic Activation and Hypertension Independent of Circulating Angiotensin II
    Authors: Dalmasso, C;Ahmed, NS;Ghuneim, S;Cincinelli, C;Leachman, JR;Giani, JF;Cassis, L;Loria, AS;
    Journal of the American Heart Association
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  2. Medium-Chain Length Fatty Acids Enhance Abeta Degradation by Affecting Insulin-Degrading Enzyme
    Authors: J Mett, AA Lauer, D Janitschke, LV Griebsch, EL Theiss, HS Grimm, H Koivisto, H Tanila, T Hartmann, MOW Grimm
    Cells, 2021-10-29;10(11):.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  3. High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity.
    Authors: Lu J, Sun P
    J Biol Chem, 2020-10-29;295(52):18579-18588.
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay
  4. Cathepsin A contributes to left ventricular remodeling by degrading extracellular superoxide dismutase in mice
    Authors: M Hohl, M Mayr, L Lang, AG Nickel, J Barallobre, X Yin, T Speer, SR Selejan, C Goettsch, K Erb, C Fecher-Tro, JC Reil, B Linz, S Ruf, T Hübschle, C Maack, M Böhm, T Sadowski, D Linz
    J. Biol. Chem., 2020-07-09;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  5. High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activity
    Authors: J Lu, PD Sun
    bioRxiv, 2020-07-01;0(0):.
    Applications: Enzyme Assay
  6. Female Mice Exposed to Postnatal Neglect Display Angiotensin II-Dependent Obesity-Induced Hypertension
    Authors: C Dalmasso, JR Leachman, CM Ensor, FB Yiannikour, JF Giani, LA Cassis, AS Loria
    J Am Heart Assoc, 2019-11-22;8(23):e012309.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Bioassay
  7. Neuroprotection induced by Navbeta2?knockdown in APP/PS1 transgenic neurons is associated with NEP regulation
    Authors: T Hu, SS Li, MN Lu, L Zhang, B Chen, R Mao, R Mei, YX Tan, S Li, YB Xiyang
    Mol Med Rep, 2019-06-20;20(2):2002-2011.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Bioassay
  8. The selectivity of galardin and an azasugar-based hydroxamate compound for human matrix metalloproteases and bacterial metalloproteases
    Authors: I Sylte, R Dawadi, N Malla, S von Hofste, TM Nguyen, AI Solli, E Berg, OA Adekoya, G Svineng, JO Winberg
    PLoS ONE, 2018-08-03;13(8):e0200237.
    Species: Bacteria
    Sample Types: Protein
  9. Functional requirement for human pitrilysin metallopeptidase 1 arginine 183, mutated in amyloidogenic neuropathy
    Authors: JE Smith-Carp, BJ Alper
    Protein Sci., 2018-02-23;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  10. The anti-oxidant xanthorrhizol prevents amyloid-?-induced oxidative modification and inactivation of neprilysin
    Authors: CS Lim, JS Han
    Biosci. Rep., 2018-02-02;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  11. Vitamin D and Its Analogues Decrease Amyloid-? (A?) Formation and Increase A?-Degradation
    Authors: MOW Grimm, A Thiel, AA Lauer, J Winkler, J Lehmann, L Regner, C Nelke, D Janitschke, C Benoist, O Streidenbe, H Stötzel, K Endres, C Herr, C Beisswenge, HS Grimm, R Bals, F Lammert, T Hartmann
    Int J Mol Sci, 2017-12-19;18(12):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Activity Assay
  12. Characterization of insulin degrading enzyme and other amyloid-beta degrading proteases in human serum: a role in Alzheimer's disease?
    Authors: Liu Z, Zhu H, Fang G, Walsh K, Mwamburi M, Wolozin B, Abdul-Hay S, Ikezu T, Leissring M, Qiu W
    Oncogene, 2012-01-01;29(2):329-40.
    Species: Human
    Sample Types: Serum
    Applications: Enzyme Assay Substrate
  13. HIV-1 reduces Abeta-degrading enzymatic activities in primary human mononuclear phagocytes.
    Authors: Lan X, Xu J, Kiyota T, Peng H, Zheng JC, Ikezu T
    J. Immunol., 2011-05-06;186(12):6925-32.
    Species: Human
    Sample Types: Cell Lysates
  14. Expression and functional profiling of neprilysin, insulin-degrading enzyme, and endothelin-converting enzyme in prospectively studied elderly and Alzheimer's brain.
    Authors: Wang S, Wang R, Chen L, Bennett DA, Dickson DW, Wang DS
    J. Neurochem., 2010-07-30;115(1):47-57.
    Species: Human
    Sample Types: Tissue Homogenates
  15. Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
    Authors: Wendt W, Schulten R, Stichel CC, Lubbert H
    J. Neurochem., 2009-07-17;110(6):1931-41.
    Species: Mouse
    Sample Types: Cell Lysates
  16. ACE mediates ventilator-induced lung injury in rats via angiotensin II but not bradykinin.
    Authors: Wosten-van Asperen RM, Lutter R, Haitsma JJ, Merkus MP, van Woensel JB, van der Loos CM, Florquin S, Lachmann B, Bos AP
    Eur. Respir. J., 2007-10-24;31(2):363-71.
    Species: Rat
    Sample Types: BALF
  17. Immunocapture-based fluorometric assay for the measurement of neprilysin-specific enzyme activity in brain tissue homogenates and cerebrospinal fluid.
    Authors: Miners JS, Verbeek MM, Rikkert MO, Kehoe PG, Love S
    J. Neurosci. Methods, 2007-08-25;167(2):229-36.
    Species: Human
    Sample Types: Tissue Homogenates
  18. Human Opiorphin, a natural antinociceptive modulator of opioid-dependent pathways.
    Authors: Wisner A, Dufour E, Messaoudi M, Nejdi A, Marcel A, Ungeheuer MN, Rougeot C
    Proc. Natl. Acad. Sci. U.S.A., 2006-11-13;103(47):17979-84.
    Species: Human
    Sample Types: Recombinant Protein

FAQs

  1. Where does Cathepsin A cleave Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate, Catalog # ES005?

    • Although the QC assay conditions provided on our recombinant Cathepsin A datasheets should favor carboxypeptidase activity, it is possible that there is more than one site in the ES005 peptide recognized and cleaved by Cathepsin A.  We have not performed verification experiments to confirm the cleavage site preferred in our reaction conditions.

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Reviews for Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate

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Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate
By Shrinidh Joshi on 08/04/2016
Mca-RPPGFSAFK(Dnp)-OH Fluorogenic Peptide Substrate ES005