Janelia Fluor® 669, NHS ester
Chemical Name: 1-[7-(1-Azetidinyl)-10-[2-Carboxy-5-[(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethyl]thio-3,4,6-trifluorophenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt
Purity: ≥90%
Biological Activity
Key Information: Janelia Fluor® 669, NHS ester is a red fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 669 nm and 682 nm, respectively; quantum yield = 0.37; extinction coefficient = 116,000 M-1cm-1; A280 correction factor = 0.043. Retains 97% fluorescence after 30 bleaching cycles.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was Janelia Fluor® 669, SE.
Scientific Data
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Applications on Tubulin for Janelia Fluor® 669, NHS ester. Application of Janelia Fluor® 669, NHS ester dye on Tubulin; Left: Microtubules were labeled with anti-alpha -tubulin primary antibodies. The secondary antibodies were conjugated to Janelia Fluor® 669, NHS ester. Maximum intensity projection of confocal z-stack, recorded with an Olympus FV1200 confocal microscope, using 638 nm laser line for JF 669. Right: Enlarged portion of left-hand image. Images kindly provided by Dr Kirstin Elgass, Monash University.
Optical Data for Janelia Fluor® 669, NHS ester
| Emission Color | Far Red |
|---|---|
| Brightness | |
| λabs | 669 nm |
| λem | 682 nm |
| Extinction Coefficient (ε) | 116,000 M-1cm-1 |
| Quantum Yield (φ) | 0.37 |
| Closest Laser line | 640 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.043 |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM) |
| Alternative For | Alexa Fluor® 660, Atto 655, Atto 665, CF660R |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
Background References
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A general method to fine-tune fluorophores for live-cell and in vivo imaging.
Grimm et al.
Nat.Methods, 2017;14:987 -
General synthetic method for Si-Fluoresceins and Si-Rhodamines.
Grimm et al.
ACS Cent.Sci., 2017;3:975 -
A general method to optimize and functionalize red-shifted rhodamine dyes.
Grimm et al.
Nat.Methods, 2020;17:815
Product Datasheets
Citation for Janelia Fluor® 669, NHS ester
The citations listed below are publications that use Tocris products. Selected citations for Janelia Fluor® 669, NHS ester include:
1 Citation: Showing 1 - 1
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EASI-FISH for thick tissue defines lateral hypothalamus spatio-molecular organization.
Authors: Hui Et al.
Cell 2021;184:6361-6377.e24
FAQs
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