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Insect Cathepsin L Antibody

Catalog #: MAB2259
2 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB2259
MAB2259-SP
Product Details
Citations (2)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Insect Cathepsin L Antibody Summary

Species Reactivity
Insect
Specificity
Detects pro form of Insect Cathepsin L in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Cathepsin S is observed and no cross-reactivity with rhCathepsin B, F, K, L2/V, and activated, mature insect Cathepsin L is observed.
Source
Monoclonal Mouse IgG2A Clone # 193720
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda ovarian cell line Sf 21-derived Insect Cathepsin L
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Insect Cathepsin L
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Insect Cathepsin L, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin L

The pro form of insect cell Cathepsin L is stable at neutral pH, but autoactivates in acidic buffers under reducing conditions. The activated enzyme is similar in substrate specificity to mammalian Cathepsin L, and is very active as a protease, as judged by its ability to degrade azocasein. Cystatins A, B, C, E/M, F, SA, and SN are potent inhibitors of the insect cell Cathepsin L. Like other cysteine proteases related to papain, insect cell Cathepsin L is also inhibited by E-64 and reagents which react with thiols.

References
  1. Johnson, G.D. and W. Jiang (2005) Arch. Biochem. Biophys.444:7.
Entrez Gene IDs
1514 (Human); 13039 (Mouse); 118276442 (Insect)
Alternate Names
Cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 3.4.22.15; EC 3.4.22; FLJ31037; Major excreted protein; MEP

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Citations for Insect Cathepsin L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Genetic analysis of the Drosophila ESCRT-III complex protein, VPS24, reveals a novel function in lysosome homeostasis
    Authors: JR Florian, SJ DeMatte, DM Sweeder, RW Ordway, F Kawasaki
    PLoS ONE, 2021-05-06;16(5):e0251184.
    Species: Drosophila
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Characterization of cathepsin L secreted by Sf21 insect cells.
    Authors: Johnson GD, Jiang W
    Arch. Biochem. Biophys., 2005-10-21;444(1):7-14.
    Species: Insect
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation, Western Blot

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