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Hyaluronan Quantikine ELISA Kit

Catalog #: DHYAL0
76 Citations 14 Reviews Datasheet
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DHYAL0
PDHYAL0
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QC104: Quantikine Immunoassay Control Set 997 for Hyaluronan
Human, Mouse, Rat, Porcine, Canine Hyaluronan ELISA Standard Curve
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Citations (76)
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Reviews (14)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Hyaluronan Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
0.2 ng/mL
Assay Range
0.6 - 40 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Measures greater than or equal to 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Hyaluronan Immunoassay is a 4.5 hour solid-phase ELISA designed to measure at least 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine cell culture supernates, tissue homogenates, serum, and plasma samples. It contains Streptococcus pyogenes-derived Hyaluronan and NS0-expressed human Aggrecan. This immunoassay has been shown to accurately quantitate Hyaluronan.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 1.53 4.81 14.3 1.68 4.68 14.98
Standard Deviation 0.11 0.26 0.52 0.08 0.23 0.72
CV% 7.2 5.4 3.6 4.8 4.9 4.8

Recovery

The recovery of Hyaluronan spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 104 96-116
EDTA Plasma (n=10) 97 83-106
Heparin Plasma (n=10) 99 80-124
Serum (n=10) 98 81-119

Linearity

To assess the linearity of the assay, samples containing high concentrations of Hyaluronan were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Hyaluronan ELISA Linearity
Mouse Hyaluronan ELISA Linearity
Rat Hyaluronan ELISA Linearity
Porcine Hyaluronan ELISA Linearity
Canine Hyaluronan ELISA Linearity

Scientific Data

N/A Human, Mouse, Rat, Porcine, Canine Hyaluronan ELISA Standard Curve View Larger

Human, Mouse, Rat, Porcine, Canine Hyaluronan ELISA Standard Curve

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Product Datasheet

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Hyaluronan

Hyaluronan, also known as hyaluronic acid (HA) or sodium hyaluronate, is a naturally occurring linear polymer. Hyaluronan is a glycosaminoglycan (GAG) that is ubiquitously present in the extracellular matrix of all vertebrates and is also present in the capsule of some strains of Streptococci. Mammalian hyaluronan is synthesized by one of three distinct hyaluronan synthases (HAS1, 2, and 3), which produce HA polymers with different chain lengths and differ in their rates of synthesis. Whereas high molecular weight HA (> 500 kDa) is anti-angiogenic, anti-inflammatory and immunosuppressive, low molecular weight HA (10 - 500 kDa) is highly angiogenic and pro-inflammatory. HA oligomers are anti-apoptotic and upregulate heat shock protein expression. Functionally, hyaluronan molecules are important for the maintenance of a highly hydrated extracellular matrix in tissues, which is involved in cell adhesion and supports cell migration.

Alternate Names:
Hyaluronan
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Hyaluronan Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

76 Citations: Showing 1 - 10
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  1. Synovium friction properties are influenced by proteoglycan content
    Authors: Gangi, LR;Pagon, AD;Pellicore, MJ;Kroupa, KR;Murphy, LA;Ateshian, GA;Hung, CT;
    Journal of biomechanics
    Species: Bovine, Human
    Sample Types: Tissue Homogenates
  2. CD44 signaling in Müller cells impacts photoreceptor function and survival in healthy and diseased retinas
    Authors: Ayten, M;Straub, T;Kaplan, L;Hauck, SM;Grosche, A;Koch, SF;
    Journal of neuroinflammation
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
  3. Hyaluronan Mediates Cold-Induced Adipose Tissue Beiging
    Authors: Chen, X;Wang, Y;Li, H;Deng, Y;Giang, C;Song, A;Liu, Y;Wang, QA;Zhu, Y;
    Cells
    Species: Transgenic Mouse
    Sample Types: Serum, Tissue Homogenates
  4. Pressure sensing of lysosomes enables control of TFEB responses in macrophages
    Authors: Cai, R;Scott, O;Ye, G;Le, T;Saran, E;Kwon, W;Inpanathan, S;Sayed, BA;Botelho, RJ;Saric, A;Uderhardt, S;Freeman, SA;
    Nature cell biology
    Species: Human
    Sample Types: Cell Lysates
  5. Vocal fold fibroblasts and exposure to vibration in vitro: Does sex matter?
    Authors: Kirsch, A;Grossmann, T;Steffan, B;Groselj-Strele, A;Gerstenberger, C;Gugatschka, M;
    PloS one
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Dietary Isoeugenol Supplementation Attenuates Chronic UVB-Induced Skin Photoaging and Modulates Gut Microbiota in Mice
    Authors: Geng, R;Kang, SG;Huang, K;Tong, T;
    Nutrients
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Targeting glioblastoma tumor hyaluronan to enhance therapeutic interventions that regulate metabolic cell properties
    Authors: Neves, ER;Anand, A;Mueller, J;Remy, RA;Xu, H;Selting, KA;Sarkaria, JN;Harley, BA;Pedron-Haba, S;
    bioRxiv : the preprint server for biology
    Species: Xenograft
    Sample Types: Cell Culture Supernates
  8. Anti-Aging Potential of a Novel Ingredient Derived from Sugarcane Straw Extract (SSE)
    Authors: Carvalho, MJ;Pedrosa, SS;Mendes, A;Azevedo-Silva, J;Fernandes, J;Pintado, M;Oliveira, ALS;Madureira, AR;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Cumulus expansion is impaired with advanced reproductive age due to loss of matrix integrity and reduced hyaluronan
    Authors: Babayev, E;Suebthawinkul, C;Gokyer, D;Parkes, WS;Rivas, F;Pavone, ME;Hall, AR;Pritchard, MT;Duncan, FE;
    Aging cell
    Species: Human
    Sample Types: Follicular Fluid
  10. CD44?hyaluronan axis plays a role in the interactions between colon cancer?derived extracellular vesicles and human monocytes
    Authors: Babula, A;Ga?uszka-Bulaga, A;W?glarczyk, K;Siedlar, M;Baj-Krzyworzeka, M;
    Oncology letters
    Species: Human
    Sample Types: Cell Culture Supernates, Extracellular Vesicles
  11. Aqueous extract of Cordyceps cicadae (Miq.) promotes hyaluronan synthesis in human skin fibroblasts: A potential moisturizing and anti-aging ingredient
    Authors: Shao, L;Jiang, S;Li, Y;Yu, L;Liu, H;Ma, L;Yang, S;
    PloS one
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Biocompatible topical delivery system of high-molecular-weight hyaluronan into human stratum corneum using magnesium chloride
    Authors: Fujii, MY;Okishima, A;Ichiwata, HS;Oka, T;
    Scientific reports
    Species:  Human
    Sample Types: Whole Tissue
  13. Protective effect of Schizonepeta tenuifolia Briq. ethanolic extract against UVB-induced skin aging and photodamage in hairless mice
    Authors: Gu, MJ;Lee, HW;Yoo, G;Kim, D;Choi, IW;Kim, Y;Ha, SK;
    Frontiers in pharmacology
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. Glycan Node Analysis Detects Varying Glycosaminoglycan Levels in Melanoma-Derived Extracellular Vesicles
    Authors: Pendiuk Goncalves, J;Walker, SA;Aguilar Diaz de Leon, JS;Yang, Y;Davidovich, I;Busatto, S;Sarkaria, J;Talmon, Y;Borges, CR;Wolfram, J;
    International journal of molecular sciences
  15. alpha-Ionone protects against UVB-induced photoaging in epidermal keratinocytes
    Authors: R Geng, SG Kang, K Huang, T Tong
    Chinese herbal medicines, 2022-11-09;15(1):132-138.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Use of radiolabeled hyaluronic acid for preclinical assessment of inflammatory injury and acute respiratory distress syndrome
    Authors: F Zhao, CJ Barber, S Sammani, L Wan, BW Miller, LR Furenlid, Z Li, DB Gotur, R Barrios, JM Woolfenden, DR Martin, Z Liu
    Nuclear medicine and biology, 2022-10-14;114(0):86-98.
    Species: Mouse
    Sample Types: Cell Lysates
  17. Effect of CD44 signal axis in the gain of mesenchymal stem cell surface antigens from synovial fibroblasts in vitro
    Authors: M Isono, J Takeuchi, A Maehara, Y Nakagawa, H Katagiri, K Miyatake, I Sekiya, H Koga, Y Asou, K Tsuji
    Heliyon, 2022-09-28;8(10):e10739.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Characterization of Lung Injury following Abrin Pulmonary Intoxication in Mice: Comparison to Ricin Poisoning
    Authors: A Sapoznikov, Y Gal, R Alcalay, Y Evgy, T Sabo, C Kronman, R Falach
    Toxins, 2022-09-02;14(9):.
    Species: Mouse
    Sample Types: BALF
  19. The spectrin cytoskeleton integrates endothelial mechanoresponses
    Authors: S Mylvaganam, J Plumb, B Yusuf, R Li, CY Lu, LA Robinson, SA Freeman, S Grinstein
    Nature Cell Biology, 2022-07-11;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. In vitro assessment of the anti-inflammatory and skin-moisturizing effects of Filipendula palmata (Pall.) Maxim. On human keratinocytes and identification of its bioactive phytochemicals
    Authors: XJ Mi, JK Kim, S Lee, SK Moon, YJ Kim, H Kim
    Journal of ethnopharmacology, 2022-07-07;296(0):115523.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Human placental extract activates a wide array of gene expressions related to skin functions
    Authors: PY Chang, LC Chin, K Kimura, Y Nakahata
    Scientific Reports, 2022-06-30;12(1):11031.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Disulfiram Exerts Antifibrotic and Anti-Inflammatory Therapeutic Effects on Perimysial Orbital Fibroblasts in Graves' Orbitopathy
    Authors: X Wang, H Ye, S Yang, X Sha, X Wang, T Zhang, R Chen, W Xiao, H Yang
    International Journal of Molecular Sciences, 2022-05-09;23(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Plasma proteome changes linked to late phase response after inhaled allergen challenge in asthmatics
    Authors: M Weitoft, M Kadefors, H Stenberg, E Tufvesson, Z Diamant, S Rolandsson, L Bjermer, O Rosmark, G Westergren
    Respiratory Research, 2022-03-05;23(1):50.
    Species: Human
    Sample Types: Plasma
  24. Associations between serum biomarkers of cartilage metabolism and serum hyaluronic acid, with risk factors, pain categories, and disease severity in knee osteoarthritis: a pilot study
    Authors: C Papaneophy, A Alabajos-C, E Viosca-Her, C Calvis, M Costa, AE Christodou, A Kroushovsk, A Lapithis, VM Lapithi, I Papayianni, A Christou, R Messeguer, C Giannaki, K Felekkis
    Bmc Musculoskeletal Disorders, 2022-03-02;23(1):195.
    Species: Human
    Sample Types: Serum
  25. Association between plasma glycocalyx component levels and poor prognosis in severe influenza type A (H1N1)
    Authors: X Huang, F Lu, H Tian, H Hu, F Ning, Q Shang, D Hao, W Zhu, G Kong, X Ma, J Feng, T Wang, X Wang
    Scientific Reports, 2022-01-07;12(1):163.
    Species: Human
    Sample Types: Plasma
  26. Intramuscular Exposure to a Lethal Dose of Ricin Toxin Leads to Endothelial Glycocalyx Shedding and Microvascular Flow Abnormality in Mice and Swine
    Authors: A Sapoznikov, Y Gal, Y Evgy, M Aftalion, S Katalan, T Sabo, C Kronman, R Falach
    International Journal of Molecular Sciences, 2021-11-16;22(22):.
    Species: Mouse, Porcine
    Sample Types: Serum
  27. Intriguing findings of liver fibrosis following COVID-19
    Authors: O Kolesova, I Vanaga, S Laivacuma, A Derovs, A Kolesovs, M Radzina, A Platkajis, J Eglite, E Hagina, S Arutjunana, DS Putrins, J Storozenko, B Rozentale, L Viksna
    BMC gastroenterology, 2021-10-11;21(1):370.
    Species: Human
    Sample Types: Serum
  28. In�vitro effects of conditioned medium from bioreactor cultured human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on skin-derived cell lines
    Authors: YM Park, M Lee, S Jeon, D Hr?zová
    Regenerative Therapy, 2021-08-24;18(0):281-291.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Adipose tissue hyaluronan production improves systemic glucose homeostasis and primes adipocytes for CL 316,243-stimulated lipolysis
    Authors: Y Zhu, N Li, M Huang, M Bartels, S Dogné, S Zhao, X Chen, C Crewe, L Straub, L Vishvanath, Z Zhang, M Shao, Y Yang, CM Gliniak, R Gordillo, GI Smith, WL Holland, RK Gupta, B Dong, N Caron, Y Xu, Y Akgul, S Klein, PE Scherer
    Nature Communications, 2021-08-10;12(1):4829.
    Species: Mouse
    Sample Types: Tissue Homogenates
  30. Hyaluronan and Collagen Are Prominent Extracellular Matrix Components in Bovine and Porcine Ovaries
    Authors: WS Parkes, F Amargant, LT Zhou, CE Villanueva, FE Duncan, MT Pritchard
    Genes, 2021-07-30;12(8):.
    Species: Bovine, Porcine
    Sample Types: Tissue Homogenates
  31. Vocal Fold Fibroblasts in Reinke's Edema Show Alterations Involved in Extracellular Matrix Production, Cytokine Response and Cell Cycle Control
    Authors: M Grill, I Lazzeri, A Kirsch, N Steurer, T Grossmann, M Karbiener, E Heitzer, M Gugatschka
    Biomedicines, 2021-06-26;9(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Oxidative damage to hyaluronan-CD44 interactions as an underlying mechanism of action of oxidative stress-inducing cancer therapy
    Authors: M Yusupov, A Privat-Mal, RM Cordeiro, H Verswyvel, P Shaw, J Razzokov, E Smits, A Bogaerts
    Redox Biology, 2021-04-11;43(0):101968.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Sargassum horneri (Turner) C. Agardh ethanol extract attenuates fine dust-induced inflammatory responses and impaired skin barrier functions in HaCaT keratinocytes
    Authors: MKHM Dias, DMD Madusanka, EJ Han, HS Kim, YJ Jeon, Y Jee, KN Kim, K Lee, IPS Fernando, G Ahn
    Journal of ethnopharmacology, 2021-03-09;273(0):114003.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Blood reelin in the progression of chronic liver disease
    Authors: L Sturm, L Roth, K Zoldan, M Schultheis, T Boettler, JP Huber, R Kaeser, R Thimme, D Bettinger
    Advances in medical sciences, 2021-02-06;66(1):148-154.
    Species: Human
    Sample Types: Plasma
  35. Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner
    Authors: W Zhang, G Yin, H Zhao, H Ling, Z Xie, C Xiao, Y Chen, Y Lin, T Jiang, S Jin, J Wang, X Yang
    Cell Death & Disease, 2021-01-20;12(1):102.
    Species: Human, Mouse
    Sample Types: Serum
  36. Newly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis
    Authors: T Iba, JH Levy, K Aihara, K Kadota, H Tanaka, K Sato, I Nagaoka
    International Journal of Molecular Sciences, 2020-12-26;22(1):.
    Species: Rat
    Sample Types: Serum
  37. In vitro mechanical vibration down-regulates pro-inflammatory and pro-fibrotic signaling in human vocal fold fibroblasts
    Authors: D Hortobagyi, T Grossmann, M Tschernitz, M Grill, A Kirsch, C Gerstenber, M Gugatschka
    PLoS ONE, 2020-11-19;15(11):e0241901.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Inhibitor of Hyaluronic Acid Synthesis 4-Methylumbelliferone as an Anti-Inflammatory Modulator of LPS-Mediated Astrocyte Responses
    Authors: DV Chistyakov, AI Nikolskaya, SV Goriainov, AA Astakhova, MG Sergeeva
    Int J Mol Sci, 2020-11-02;21(21):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  39. Essential role and therapeutic targeting of the glomerular endothelial glycocalyx in lupus nephritis
    Authors: H Kadoya, N Yu, IM Schiessl, A Riquier-Br, G Gyarmati, D Desposito, K Kidokoro, MJ Butler, CO Jacob, J Peti-Peter
    JCI Insight, 2020-10-02;0(0):.
    Species: Mouse
    Sample Types: Plasma
  40. Negative regulation between the expression levels of receptor for hyaluronic acid-mediated motility and hyaluronan leads to cell migration in pancreatic cancer
    Authors: XB Cheng, S Wang, H Yang, H Tong, G Shi, L Wu, J Zhou, L Shi, H Li, S Ren, Y Zhou, N Sato
    Oncology Letters, 2020-09-04;20(5):199.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. 11&beta?hydroxysteroid dehydrogenase?1 is associated with the activation of hepatic stellate cells in the development of hepatic fibrosis
    Authors: W Xiao, MH Lu, PF Rong, HY Zhang, J Gong, YQ Peng, HY Gong, ZG Liu
    Molecular Medicine Reports, 2020-08-07;0(0):.
    Species: Rat
    Sample Types: Serum
  42. Involvement of hyaluronan in the adaptive changes of the rat small intestine neuromuscular function after ischemia/reperfusion injury
    Authors: M Bistoletti, A Bosi, I Caon, AM Chiaravall, P Moretto, A Genoni, E Moro, E Karousou, M Viola, F Crema, A Baj, A Passi, D Vigetti, C Giaroni
    Sci Rep, 2020-07-13;10(1):11521.
    Species: Rat
    Sample Types: Cell Culture Lysates
  43. Synucleinopathy alters nanoscale organization and diffusion in the brain extracellular space through hyaluronan remodeling
    Authors: FN Soria, C Paviolo, E Doudnikoff, ML Arotcarena, A Lee, N Danné, AK Mandal, P Gosset, B Dehay, L Groc, L Cognet, E Bezard
    Nat Commun, 2020-07-10;11(1):3440.
    Species: Mouse
    Sample Types: EDTA Plasma
  44. Repeated Freezing Procedures Preserve Structural and Functional Properties of Amniotic Membrane for Application in Ophthalmology
    Authors: O Pogozhykh, N Hofmann, O Gryshkov, C von Kaisen, M Mueller, B Glasmacher, D Pogozhykh, M Börgel, R Blasczyk, C Figueiredo
    Int J Mol Sci, 2020-06-04;21(11):.
    Species: Human
    Sample Types: Tissue Homogenates
  45. Decanal Protects against UVB-Induced Photoaging in Human Dermal Fibroblasts via the cAMP Pathway
    Authors: W Kang, D Choi, T Park
    Nutrients, 2020-04-25;12(5):.
    Species: Human
    Sample Types: cell culture supernatant
  46. IGFBP-3 Blocks Hyaluronan-CD44 Signaling, Leading to Increased Acetylcholinesterase Levels in A549 Cell Media and Apoptosis in a p53-Dependent Manner
    Authors: D Price, R Muterspaug, B Clegg, A Williams, A Stephens, J Guthrie, D Heyl, HG Evans
    Sci Rep, 2020-03-19;10(1):5083.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. 4-methylumbelliferone inhibits clonogenic potency by suppressing high molecular weight-hyaluronan in fibrosarcoma cells
    Authors: K Hasegawa, R Saga, R Takahashi, R Fukui, M Chiba, K Okumura, E Tsuruga, Y Hosokawa
    Oncol Lett, 2020-02-05;19(4):2801-2808.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Sirtuin 1 reduces hyaluronan synthase 2 expression by inhibiting nuclear translocation of NF-kB and expression of the long-non coding RNA HAS2-AS1
    Authors: I Caon, B Bartolini, P Moretto, A Parnigoni, E Caravà, DL Vitale, L Alaniz, M Viola, E Karousou, G De Luca, VC Hascall, A Passi, D Vigetti
    J. Biol. Chem., 2020-01-13;0(0):.
    Species: Human
    Sample Types: Cell Culture Supern
  49. Dietary Suberic Acid Protects Against UVB-Induced Skin Photoaging in Hairless Mice
    Authors: W Kang, D Choi, T Park
    Nutrients, 2019-12-04;11(12):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  50. Proteomic atlas of organ vasculopathies triggered by Staphylococcus aureus sepsis
    Authors: AG Toledo, G Golden, AR Campos, H Cuello, J Sorrentino, N Lewis, N Varki, V Nizet, JW Smith, JD Esko
    Nat Commun, 2019-10-11;10(1):4656.
    Species: Mouse
    Sample Types: Plasma
  51. Reversal of bleomycin-induced rat pulmonary fibrosis by a xenograft of human umbilical mesenchymal stem cells from Wharton's jelly
    Authors: KA Chu, SY Wang, CC Yeh, TW Fu, YY Fu, TL Ko, MM Chiu, TH Chen, PJ Tsai, YS Fu
    Theranostics, 2019-09-17;9(22):6646-6664.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Differential Consequences of Bmp9 Deletion on Sinusoidal Endothelial Cell Differentiation and Liver Fibrosis in 129/Ola and C57BL/6 Mice
    Authors: A Desroches-, E Tillet, N Ricard, M Ouarné, C Mallet, JJ Feige, S Bailly
    Cells, 2019-09-13;8(9):.
    Species: Mouse
    Sample Types: Plasma
  53. Hyaluronan and LYVE-1 and allograft function in lung transplantation recipients
    Authors: AM Courtwrigh, AM Lamattina, PH Louis, AJ Trindade, P Burkett, J Imani, S Shrestha, M Divo, S Keller, IO Rosas, HJ Goldberg, S El-Chemaly
    Sci Rep, 2019-06-21;9(1):9003.
    Species: Human
    Sample Types: Serum
  54. ?-Ionone Protects Against UVB-Induced Photoaging in Human Dermal Fibroblasts
    Authors: T Tong, J Park, Y Moon, W Kang, T Park
    Molecules, 2019-05-09;24(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Synovium extra cellular matrices seeded with transduced mesenchymal stem cells stimulate chondrocyte maturation in vitro and cartilage healing in clinically-induced rat-knee lesions in vivo
    Authors: NA Reisbig, E Pinnell, L Scheuerman, H Hussein, AL Bertone
    PLoS ONE, 2019-03-12;14(3):e0212664.
    Species: Equine
    Sample Types: Cell Culture Supernates
  56. Impact of Lymphangiogenesis on Cardiac Remodeling After Ischemia and Reperfusion Injury
    Authors: Y Shimizu, R Polavarapu, KL Eskla, Y Pantner, CK Nicholson, M Ishii, D Brunnhoelz, R Mauria, A Husain, N Naqvi, T Murohara, JW Calvert
    J Am Heart Assoc, 2018-10-02;7(19):e009565.
    Species: Mouse
    Sample Types: Tissue Homogenates
  57. In vitro elucidation of the role of pericellular matrix in metastatic extravasation and invasion of breast carcinoma cells
    Authors: ME Brett, HE Bomberger, GR Doak, MA Price, JB McCarthy, DK Wood
    Integr Biol (Camb), 2018-04-23;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. Fetal extracellular matrix nerve wraps locally improve peripheral nerve remodeling after complete transection and direct repair in rat
    Authors: T Ren, A Faust, Y van der Me, B Xiao, S Johnson, A Kandakatla, VS Gorantla, SF Badylak, KM Washington, MB Steketee
    Sci Rep, 2018-03-14;8(1):4474.
    Species: Porcine
    Sample Types: Tissue Homogenates
  59. Modulation of hyaluronan polymer size regulates proliferation of perimysial fibroblasts in thyroid eye disease
    Authors: R Ma, Q Li, Z Wang, Y Yuan, L Gan, J Qian
    Biochem. Biophys. Res. Commun., 2018-02-05;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  60. Transmembrane Pickets Connect Cyto- and Pericellular Skeletons Forming Barriers to Receptor Engagement
    Authors: SA Freeman, A Vega, M Riedl, RF Collins, PP Ostrowski, EC Woods, CR Bertozzi, MI Tammi, DS Lidke, P Johnson, S Mayor, K Jaqaman, S Grinstein
    Cell, 2018-01-11;172(1):305-317.e10.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  61. Cactus cladodes (Opuntia humifusa) extract minimizes the effects of UV irradiation on keratinocytes and hairless mice
    Authors: K Park, HS Choi, YH Hong, EY Jung, HJ Suh
    Pharm Biol, 2017-12-01;55(1):1032-1040.
    Species: Mouse
    Sample Types: Tissue Homogenates
  62. Cervical HSV-2 infection causes cervical remodeling and increases risk for ascending infection and preterm birth
    Authors: D McGee, A Smith, S Poncil, A Patterson, AI Bernstein, K Racicot
    PLoS ONE, 2017-11-30;12(11):e0188645.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates, Cervical Fluid
  63. Up-regulation of Interleukin-21 Contributes to Liver Pathology of Schistosomiasis by Driving GC Immune Responses and Activating HSCs in Mice
    Authors: Y Wang, C Lin, Y Cao, Z Duan, Z Guan, J Xu, XQ Zhu, C Xia
    Sci Rep, 2017-11-30;7(1):16682.
    Species: Mouse
    Sample Types: Serum
  64. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
    Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
    Sci Rep, 2017-08-16;7(1):8484.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. CD4+ T lymphocytes produce adiponectin in response to transplants
    Authors: S Danturti, KS Keslar, LR Steinhoff, R Fan, N Dvorina, A Valujskikh, RL Fairchild, WM Baldwin
    JCI Insight, 2017-06-15;2(12):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  66. A prognostic model for development of significant liver fibrosis in HIV-hepatitis C co-infection
    Authors: N Moqueet, C Kanagarath, MJ Gill, M Hull, S Walmsley, D Radzioch, S Saeed, RW Platt, MB Klein
    PLoS ONE, 2017-05-03;12(5):e0176282.
    Species: Human
    Sample Types: Serum
  67. Association of Endothelial Glycocalyx and Tight and Adherens Junctions With Severity of Plasma Leakage in Dengue Infection
    Authors: S Suwarto, RT Sasmono, R Sinto, E Ibrahim, M Suryamin
    J. Infect. Dis., 2017-03-15;215(6):992-999.
    Species: Human
    Sample Types: Serum
  68. Linking the Transcriptional Landscape of Bone Induction to Biomaterial Design Parameters
    Authors: Nathalie Groen
    Adv. Mater. Weinheim, 2016-12-19;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  69. Serum levels of endothelial glycocalyx constituents in women at 20?weeks' gestation who later develop gestational diabetes mellitus compared to matched controls: a pilot study
    BMJ Open, 2016-12-15;6(12):e011244.
    Species: Human
    Sample Types: Serum
  70. Anti-tumor and anti-invasion effects of a combination of 4-methylumbelliferone and ionizing radiation in human fibrosarcoma cells
    Authors: R Saga, S Monzen, M Chiba, H Yoshino, T Nakamura, Y Hosokawa
    Oncol Lett, 2016-11-15;13(1):410-416.
    Species: Human
    Sample Types: Cell Culture Supernates
  71. Loss of the endothelial glycocalyx is associated with increased E-selectin mediated adhesion of lung tumour cells to the brain microvascular endothelium.
    Authors: Rai S, Nejadhamzeeigilani Z, Gutowski N, Whatmore J
    J Exp Clin Cancer Res, 2015-09-25;34(0):105.
    Species: Human
    Sample Types: Cell Culture Supernates
  72. Sustained Isoprostane E2 Elevation, Inflammation and Fibrosis after Acute Ischaemia-Reperfusion Injury Are Reduced by Pregnane X Receptor Activation.
    Authors: Amer A, Probert P, Dunn M, Knight M, Vallance A, Flecknell P, Oakley F, Cameron I, White S, Blain P, Wright M
    PLoS ONE, 2015-08-24;10(8):e0136173.
    Species: Rat
    Sample Types: Serum
  73. Endothelial glycocalyx shedding and vascular permeability in severely injured trauma patients.
    Authors: Rahbar E, Cardenas J, Baimukanova G, Usadi B, Bruhn R, Pati S, Ostrowski S, Johansson P, Holcomb J, Wade C
    J Transl Med, 2015-04-12;13(0):117.
    Species: Human
    Sample Types: Plasma
  74. Identification of aortic arch-specific quantitative trait loci for atherosclerosis by an intercross of DBA/2J and 129S6 apolipoprotein E-deficient mice.
    Authors: Kayashima Y, Makhanova N, Matsuki K, Tomita H, Bennett B, Maeda N
    PLoS ONE, 2015-02-17;10(2):e0117478.
    Species: Mouse
    Sample Types: Plasma
  75. Prognostic impact of hyaluronan and its regulators in pancreatic ductal adenocarcinoma.
    Authors: Cheng, Xiao-Bo, Sato, Norihiro, Kohi, Shiro, Yamaguchi, Koji
    PLoS ONE, 2013-11-11;8(11):e80765.
    Species: Human
    Sample Types: Tissue Homogenates
  76. Molecular mass dependence of hyaluronan detection by sandwich ELISA-like assay and membrane blotting using biotinylated hyaluronan binding protein.
    Authors: Yuan, Han, Tank, Mihir, Alsofyani, Abeer, Shah, Naman, Talati, Nishant, LoBello, Jaclyn C, Kim, Jin Ryou, Oonuki, Yoji, de la Motte, Carol A, Cowman, Mary K
    Glycobiology, 2013-08-19;23(11):1270-80.

FAQs

  1. Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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Hyaluronan Quantikine ELISA Kit
By Anonymous on 07/14/2022
Sample Tested: Platelet-poor EDTA Plasma
Hyaluronan Quantikine ELISA Kit DHYAL0
Hyaluronan Quantikine ELISA Kit
By Anonymous on 06/20/2022
Sample Tested: Serum-free Cell Culture Media
Hyaluronan Quantikine ELISA Kit
By Khuynh Nguyen on 04/16/2022
Sample Tested: 4T1 mouse breast cancer cell line
Hyaluronan Quantikine ELISA Kit DHYAL0
Hyaluronan Quantikine ELISA Kit
By Khuynh Nguyen on 04/16/2022
Sample Tested: MDA-MB-231 human breast cancer cell line
Hyaluronan Quantikine ELISA Kit DHYAL0
Hyaluronan Quantikine ELISA Kit
By James AHODANTIN on 01/08/2022
Sample Tested: Human plasmas,Plasmas from mice fed with HFD
Hyaluronan Quantikine ELISA Kit DHYAL0
Hyaluronan Quantikine ELISA Kit
By Anonymous on 11/22/2021
Sample Tested: human floxp embryonal stem cell culture medium
Hyaluronan Quantikine ELISA Kit DHYAL0
Hyaluronan Quantikine ELISA Kit
By Timothy Audam on 10/20/2020
Sample Tested: Heart tissue,L-929 mouse fibroblast cell line
Hyaluronan Quantikine ELISA Kit
By Anonymous on 07/17/2020
Sample Tested: Serum
Hyaluronan Quantikine ELISA Kit DHYAL0
Hyaluronan Quantikine ELISA Kit
By Jim Hsiao on 04/23/2018
Sample Tested: mouse serum
Hyaluronan Quantikine ELISA Kit DHYAL0
Hyaluronan Quantikine ELISA Kit
By Xiaoyuan Yang on 09/27/2017
Sample Tested: Human cell conditioned medium
Hyaluronan Quantikine ELISA Kit DHYAL0
Hyaluronan Quantikine ELISA Kit
By Vasken Parsekhian on 07/26/2017
Sample Tested: Serum
Hyaluronan Quantikine ELISA Kit DHYAL0
Hyaluronan Quantikine ELISA Kit
By Anonymous on 05/16/2017
Sample Tested: Adipose tissue,collagen based tissue
Hyaluronan Quantikine ELISA Kit DHYAL0
Hyaluronan Quantikine ELISA Kit
By Mike Ehrhardt on 04/24/2017
Sample Tested: Bronchoalveolar lavage
Hyaluronan Quantikine ELISA Kit DHYAL0
Hyaluronan Quantikine ELISA Kit
By Anonymous on 04/22/2017
Sample Tested: Serum and Plasma