Human XL Cytokine Luminex® Performance Base Kit

Catalog #: LUXLM000B Datasheet

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Human XL Cytokine Luminex Panel Standard Curves

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Human XL Cytokine Luminex® Performance Base Kit Summary

Panel	Luminex Performance Human XL Cytokine Panel
Assay Type	Magnetic bead-based multiplex assay for the Luminex^® platform
Format	1 x 96-well microplate and Magnetic antibody-coated beads
Species	Human
Analytes Detected	Please see analyte list in assay customization tool below.
Performance Validation	Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Make your selections below to configure your assay

Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

Premixed

User Mixed

Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split

Split based on serum

Split based on plasma

Split based on cell culture supernates

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Ordering Tool. We also offer Custom Luminex Services

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile Human Luminex Assay Base Kits with up to analytes of your choice in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine^® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Base Kit Components

Premixed cocktail of biotinylated detection antibody
Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**
High and low controls with Control Mean Value Card

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Bead Set Contents

User-mix antibody-coated Magnetic beads in individual vials

Precision

	n=40 (20x2 levels)	n=30
Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 30 different serum assays.
Analyte	Intra-Assay (%CV)	Inter-Assay (%CV)
CD40 Ligand	3.8	10.8
EGF	4.1	8.0
Eotaxin	4.8	7.4
FGF basic	2.0	7.6
Flt-3 Ligand	2.4	8.4
G-CSF	1.8	7.0
GM-CSF	3.4	11.2
Granzyme B	2.9	8.2
GRO alpha	1.9	5.6
GRO beta	1.9	6.8
IFN-alpha 2	3.6	10.0
IFN-beta	2.4	7.1
IFN-gamma	2.3	10.9
IL-1 alpha	5.0	10.6
IL-1 beta	2.8	10.1
IL-1ra	3.6	12.4
IL-2	3.9	9.6
IL-3	3.6	9.9
IL-4	2.4	8.4
IL-5	1.8	8.3
IL-6	2.2	8.8
IL-7	2.8	9.6
IL-8	3.2	84
IL-9	6.2	10.3
IL-10	3.6	11.2
IL-12 p70	2.6	9.8
IL-13	2.5	8.3
IL-15	3.2	11.2
IL-17A	2.5	9.8
IL-17E	3.1	8.1
IL-33	2.9	9.7
IP-10	3.3	6.6
MCP-1	1.8	6.3
MIP-1 alpha	2.9	5.7
MIP-1 beta	1.7	7.9
MIP-3 alpha	3.0	9.4
MIP-3 beta	2.5	8.6
PDGF-AA	2.7	7.1
PDGF-AB/BB	2.2	7.4
PD-L1/B7-H1	2.7	8.3
RANTES	1.8	7.5
TGF-alpha	4.4	9.2
TNF-alpha	3.0	10.3
TNF-beta	2.2	8.0
TRAIL	2.4	7.9
VEGF	2.9	10.1

Accuracy

	Linearity		Spiked Recovery
Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
Analyte	N	Average %	N	Average %
*Natural linearity HE = Spiked recovery was not observed due to natural linearity in high endogenous samples
CD40 Ligand	4	131	4	75
EGF*	4	150	HE
Eotaxin	4	68	4	156
FGF basic	4	128	4	87
Flt-3 Ligand	4	119	4	78
G-CSF	4	208	4	34
GM-CSF	4	101	4	98
Granzyme B	4	107	4	96
GRO alpha	4	97	4	139
GRO beta*	4	88	HE
IFN-alpha 2	4	106	4	87
IFN-beta	4	137	4	63
IFN-gamma	4	127	4	73
IL-1 alpha	4	143	3	74
IL-1 beta	4	106	4	94
IL-1ra*	4	126	HE
IL-2	4	115	4	88
IL-3	4	123	4	78
IL-4	4	141	4	58
IL-5	4	113	4	88
IL-6	4	126	4	78
IL-7	4	110	4	90
IL-8	4	118	4	78
IL-9	4	111	4	96
IL-10	4	117	4	90
IL-12 p70	4	111	4	87
IL-13	4	118	4	89
IL-15	4	103	4	76
IL-17A	4	128	4	72
IL-17E	4	150	4	55
IL-33	4	103	4	87
IP-10	4	124	4	72
MCP-1*	4	80	HE
MIP-1 alpha	4	95	4	111
MIP-1 beta	4	109	4	95
MIP-3 alpha	4	115	4	99
MIP-3 beta	4	123	4	76
PDGF-AA*	4	91	HE
PDGF-AB/BB*	4	108	HE
PD-L1/B7-H1	4	106	4	96
RANTES*	4	106	HE
TGF-alpha	4	142	4	80
TNF-alpha	4	134	4	71
TNF-beta	4	131	4	65
TRAIL	4	144	4	69
VEGF*	4	129	HE

Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex^® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or Bio-Rad^® Bio-Plex^®, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.