Human XIAP Antibody

Catalog #: MAB8223 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB8223-100
MAB8223-SP

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Human XIAP Antibody Summary

Species Reactivity

Human

Specificity

Detects human XIAP in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 117333

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human XIAP
Met1-Ser497
Accession # P98170

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human XIAP Monoclonal Antibody(Catalog # MAB8222).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Total XIAP DuoSet IC ELISA Kit (Catalog # DYC822-2) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human XIAP ELISA Standard Curve. Recombinant Human XIAP protein was serially diluted 2-fold and captured by Mouse Anti-Human XIAP Monoclonal Antibody(Catalog # MAB8222) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human XIAP Monoclonal Antibody (Catalog # MAB8223) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XIAP

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits Caspase-3 and Caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Long Name

X-linked Inhibitor of Apoptosis

Entrez Gene IDs

331 (Human); 11798 (Mouse); 63879 (Rat)

Alternate Names

API3X-linked inhibitor of apoptosis protein; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4; BIRC4; BIRC4XLP2; E3 ubiquitin-protein ligase XIAP; EC 6.3.2.-; hIAP3; hIAP-3; hILP; IAP3; IAP-3; IAP-like protein; ILP; ILP1; Inhibitor of apoptosis protein 3; MIHA; XIAP; X-linked IAP; X-linked inhibitor of apoptosis

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