Human XIAP Antibody

Catalog #: MAB8222 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB8222-100
MAB8222-SP

1 Image

All XIAP Antibodies

Product Details

FAQs

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human XIAP Antibody Summary

Species Reactivity

Human

Specificity

Detects human XIAP in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 117305

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human XIAP
Met1-Ser497
Accession # P98170

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human XIAP Monoclonal Antibody(Catalog # MAB8223).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Total XIAP DuoSet IC ELISA Kit (Catalog # DYC822-2) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

View Larger

Human XIAP ELISA Standard Curve. Recombinant Human XIAP protein was serially diluted 2-fold and captured by Mouse Anti-Human XIAP Monoclonal Antibody (Catalog # MAB8222) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human XIAP Monoclonal Antibody(Catalog # MAB8223) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XIAP

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits Caspase-3 and Caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Long Name

X-linked Inhibitor of Apoptosis

Entrez Gene IDs

331 (Human); 11798 (Mouse); 63879 (Rat)

Alternate Names

API3X-linked inhibitor of apoptosis protein; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4; BIRC4; BIRC4XLP2; E3 ubiquitin-protein ligase XIAP; EC 6.3.2.-; hIAP3; hIAP-3; hILP; IAP3; IAP-3; IAP-like protein; ILP; ILP1; Inhibitor of apoptosis protein 3; MIHA; XIAP; X-linked IAP; X-linked inhibitor of apoptosis

Product Datasheets

Product Datasheet

Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human XIAP Antibody

There are currently no reviews for this product. Be the first to review Human XIAP Antibody and earn rewards!

Have you used Human XIAP Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review