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Human VEGFR2/KDR/Flk-1 APC-conjugated Antibody

Catalog #: FAB357A
28 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
FAB357A-100
FAB357A-025
Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry.
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Product Details
Citations (28)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR2/KDR/Flk-1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 89106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2/KDR/Flk-1
Ala20-Glu764
Accession # P35968
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VEGF R2/KDR/Flk-1 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk-1 APC-conjugated Mono-clonal Antibody (Catalog # FAB357A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VEGFR2/KDR/Flk-1

VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGF R2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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Citations for Human VEGFR2/KDR/Flk-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Role of Asymmetric Methylarginine and Connexin 43 in the Regulation of Pulmonary Endothelial Function
    Authors: Hilda Tsang, James Leiper, Ka Hou Hou Lao, Laura Dowsett, Matthew W. Delahaye, Gareth Barnes et al.
    Pulmonary Circulation
  2. CXCL5 suppression recovers neovascularization and accelerates wound healing in diabetes mellitus
    Authors: Chen, C;Lin, LY;Chen, JW;Chang, TT;
    Cardiovascular diabetology
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Upregulation of PCED1B-AS1 in proliferative diabetic retinopathy and its involvement in retinal vascular endothelial cell proliferation
    Authors: X Wang, W Chen, W Lao, Y Chen
    BMC ophthalmology, 2022-11-23;22(1):450.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Melatonin Improves Ischemia-Induced Circulation Recovery Impairment in Mice with Streptozotocin-Induced Diabetes by Improving the Endothelial Progenitor Cells Functioning
    Authors: CS Kuo, CY Chen, HL Huang, HY Tsai, RH Chou, JH Wei, PH Huang, SJ Lin
    International Journal of Molecular Sciences, 2022-08-30;23(17):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. A new hemostatic agent composed of Zn2+-enriched Ca2+ alginate activates vascular endothelial cells in vitro and promotes tissue repair in vivo
    Authors: AC Ponsen, R Proust, S Soave, F Mercier-No, I Garcin, L Combettes, JJ Lataillade, G Uzan
    Bioactive materials, 2022-02-12;18(0):368-382.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Adipose stem cell niche reprograms the colorectal cancer stem cell metastatic machinery
    Authors: S Di Franco, P Bianca, DS Sardina, A Turdo, M Gaggianesi, V Veschi, A Nicotra, LR Mangiapane, M Lo Iacono, I Pillitteri, S van Hooff, F Martorana, G Motta, E Gulotta, VL Lentini, E Martorana, ME Fiori, S Vieni, MR Bongiorno, G Giannone, D Giuffrida, L Memeo, L Colarossi, M Mare, P Vigneri, M Todaro, R De Maria, JP Medema, G Stassi
    Nature Communications, 2021-08-18;12(1):5006.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Residual alterations of cardiac and endothelial function in patients who recovered from Takotsubo cardiomyopathy
    Authors: L Zilberman, A Zalik, I Fugenfirov, S Shimoni, J George, S Goland
    Clinical cardiology, 2021-05-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Compositional change of gut microbiome and osteocalcin expressing endothelial progenitor cells in patients with coronary artery disease
    Authors: T Toya, I Ozcan, MT Corban, JD Sara, EV Marietta, A Ahmad, IE Horwath, DL Loeffler, JA Murray, LO Lerman, A Lerman
    PLoS ONE, 2021-03-25;16(3):e0249187.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Monocytes as Endothelial Progenitor Cells (EPCs), Another Brick in the Wall to Disentangle Tumor Angiogenesis
    Authors: F Lopes-Coel, F Silva, S Gouveia-Fe, C Martins, N Lopes, G Domingues, C Brito, AM Almeida, SA Pereira, J Serpa
    Cells, 2020-01-01;9(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. The relationship between endothelial progenitor cells and pulmonary arterial hypertension in children with congenital heart disease
    Authors: HX Sun, GJ Li, ZH Du, Z Bing, ZX Ji, G Luo, SL Pan
    BMC Pediatr, 2019-12-17;19(1):502.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Activation of heme oxygenase-1 by Ginkgo biloba extract differentially modulates endothelial and smooth muscle-like progenitor cells for vascular repair
    Authors: TC Wu, JS Chen, CH Wang, PH Huang, FY Lin, LY Lin, SJ Lin, JW Chen
    Sci Rep, 2019-11-21;9(1):17316.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Predictive value of Type D personality for impaired endothelial function in patients with coronary artery disease
    Authors: J Denollet, RA van Felius, P Lodder, PM Mommerstee, I Goovaerts, N Possemiers, L Vanhees, P Beckers, N Pattyn, EM Van Craene
    Int. J. Cardiol., 2018-02-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. The evaluation of circulating endothelial progenitor cells and related angiogenic markers as prognostic factors in soft-tissue tumors
    Authors: I Karampinis, E Joas, A Dreyer, U Ronellenfi, J Jakob, P Hohenberge, K Nowak
    Eur J Surg Oncol, 2018-02-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Endothelial progenitor cells and plaque burden in stented coronary artery segments: an optical coherence tomography study six months after elective PCI
    Authors: S Otto, K Nitsche, C Jung, A Kryvanos, A Zhylka, K Heitkamp, JL Gutiérrez-, B Goebel, PC Schulze, HR Figulla, TC Poerner
    BMC Cardiovasc Disord, 2017-04-26;17(1):103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. The distribution and potential prognostic value of SMAD protein expression in chronic lymphocytic leukemia
    Authors: M Witkowska, A Majchrzak, B Cebula-Obr, E Wawrzyniak, T Robak, P Smolewski
    Tumour Biol, 2017-03-01;39(3):1010428317694.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Vascular CXCR4 expression promotes vessel sprouting and sensitivity to sorafenib treatment in hepatocellular carcinoma
    Authors: J Xu, J Liang, YM Meng, J Yan, XJ Yu, CQ Liu, L Xu, SM Zhuang, L Zheng
    Clin. Cancer Res, 2017-02-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Kinetics of circulating endothelial progenitor cells in patients undergoing carotid artery surgery
    Authors: Günay Kalender
    Ther Clin Risk Manag, 2016-12-14;12(0):1841-1847.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Sinoatrial node cardiomyocytes derived from human pluripotent cells function as a biological pacemaker
    Nat. Biotechnol, 2016-12-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity
    Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan
    PLoS ONE, 2016-04-04;11(4):e0152993.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Functional Assay
  20. Circulating autoantibodies to endothelial progenitor cells: binding characteristics and association with risk factors for atherosclerosis.
    Authors: George J, Matucci-Cerinic M, Bar I, Shimoni S
    PLoS ONE, 2014-06-19;9(6):e97836.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Ovine carotid artery-derived cells as an optimized supportive cell layer in 2-D capillary network assays.
    Authors: Weinandy, Stefan, Babczyk, Patrick, Dreier, Agnieszk, Unger, Ronald E, Flanagan, Thomas C, Kirkpatrick, C James, Zenke, Martin, Klee, Doris, Jockenhoevel, Stefan
    PLoS ONE, 2014-03-12;9(3):e91664.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
    Authors: Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G
    PLoS ONE, 2014-01-02;9(1):e84179.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. E-selectin mediated adhesion and migration of endothelial colony forming cells is enhanced by SDF-1alpha/CXCR4.
    Authors: Sun J, Li Y, Graziani G, Filion L, Allan D
    PLoS ONE, 2013-04-02;8(4):e60890.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. TGFbeta inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy.
    Authors: Wang C, Tang X, Sun X, Miao Z, Lv Y, Yang Y, Zhang H, Zhang P, Liu Y, DU L, Gao Y, Yin M, Ding M, Deng H
    Cell Res., 2011-08-23;22(1):194-207.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
    Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
    J. Thromb. Haemost., 2011-05-01;9(5):1056-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. VEGFR2 expressing circulating (progenitor) cell populations in volunteers and cancer patients.
    Authors: Vroling L, Yuana Y, Schuurhuis GJ, van Hinsbergh VW, Gundy C, de Haas R, van Cruijsen H, Boven E, Hoekman K, Broxterman HJ
    Thromb. Haemost., 2007-08-01;98(2):440-50.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  27. The Effect of Heparin-VEGF Multilayer on the Biocompatibility of Decellularized Aortic Valve with Platelet and Endothelial Progenitor Cells
    Authors: Xiaofeng Ye, Haozhe Wang, Jingxin Zhou, Haiqing Li, Jun Liu, Zhe Wang et al.
    PLoS ONE
  28. Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells
    Authors: Tea Soon Park, Ludovic Zimmerlin, Elias T. Zambidis
    Cytometry Part A

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Reviews for Human VEGFR2/KDR/Flk-1 APC-conjugated Antibody

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Human VEGF R2/KDR/Flk-1 APC-conjugated Antibody
By Alina Filatova on 10/26/2017
Application: Flow Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human
Flow Cytometry Human VEGF R2/KDR/Flk-1 APC-conjugated Antibody FAB357A
Human VEGF R2/KDR/Flk‑1 APC‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Cells from hematopoietic differentiation of hESC line H9
Flow Cytometry Human VEGF R2/KDR/Flk‑1 APC‑conjugated Antibody FAB357A