Home / VEGFR1/Flt-1 / Human VEGFR1/Flt‑1 Biotinylated Antibody

Human VEGFR1/Flt‑1 Biotinylated Antibody

Catalog #: BAF321
6 Citations Datasheet
Catalog # Availability Size / Price Qty
BAF321
VEGFR1/Flt‑1 in Human Breast.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR1/Flt‑1 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGFR1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse VEGFR1, recombinant human (rh) VEGFR2, and rhVEGFR3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VEGFR1/Flt-1
Ser27-His687
Accession # NP_001153392
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human VEGFR1/Flt‑1 Fc Chimera (Catalog # 321-FL)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry VEGFR1/Flt-1 antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

VEGFR1/Flt‑1 in Human Breast. VEGFR1/Flt-1 was detected in immersion fixed paraffin-embedded sections of human breast array using Human VEGFR1/Flt-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF321) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR1/Flt-1

Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Long Name
Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs
2321 (Human); 14254 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

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Citations for Human VEGFR1/Flt‑1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Low-Dose Recombinant Adeno-Associated Virus-Mediated Inhibition of Vascular Endothelial Growth Factor Can Treat Neovascular Pathologies Without Inducing Retinal Vasculitis
    Authors: Shun-Yun Cheng, Yongwen Luo, Anneliese Malachi, Jihye Ko, Qin Su, Jun Xie et al.
    Human Gene Therapy
  2. Human Umbilical Tissue-Derived Cells Secrete Soluble VEGFR1 and Inhibit Choroidal Neovascularization
    Authors: J Cao, R Yang, TE Smith, S Evans, GW McCollum, SC Pomerantz, T Petley, IR Harris, JS Penn
    Mol Ther Methods Clin Dev, 2019-05-22;14(0):37-46.
    Species: Human
    Sample Types: Recombinat Protein
    Applications: Western Blot
  3. Characteristics of the specific humoral response in patients with advanced solid tumors after active immunotherapy with a VEGF vaccine, at different antigen doses and using two distinct adjuvants
    Authors: J Sánchez Ra, Y Morera Día, M Bequet-Rom, F Hernández-, KH Selman-Hou, A de la Torr, ER Santiesteb, Y Martín Bau, CH Bermúdez B, J de la Torr, JV Gavilondo, M Ayala Avil
    BMC Immunol., 2017-07-26;18(1):39.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  4. Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease
    Sci Rep, 2016-09-23;6(0):32382.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  5. PEDF derived from glial Muller cells: a possible regulator of retinal angiogenesis.
    Authors: Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A
    Exp. Cell Res., 2004-09-10;299(1):68-78.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  6. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
    Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
    Eur. J. Clin. Invest., 2003-10-01;33(10):883-90.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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