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Human VEGFR3/Flt-4 Antibody

Catalog #: MAB3491
7 Citations 1 Review Datasheet

Discontinued Product

MAB3491 has been discontinued.
View all VEGFR3/Flt-4 products.
VEGFR3/Flt‑4 in HUVEC Human Cells.
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Product Details
Citations (7)
FAQs
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR3/Flt-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGFR3/Flt‑4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25‑30% cross‑reactivity with recombinant mouse VEGFR3 is observed and no cross-reactivity with recombinant human (rh) VEGFR1 or rhVEGFR2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 54703
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VEGFR3/Flt‑4
Tyr25-Ile776
Accession # P35916
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human VEGFR3/Flt-4 Fc Chimera (Catalog # 349-F4) under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human lung
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry VEGFR3/Flt-4 antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

VEGFR3/Flt‑4 in HUVEC Human Cells. VEGFR3/Flt-4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human VEGFR3/Flt-4 Monoclonal Antibody (Catalog # MAB3491) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR3/Flt-4

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGFR3 cDNA encodes a 1298 amino acid (aa) precursor with a 24 aa signal peptide. Mature VEGFR3 is composed of a 751 aa extracellular domain, a 22 aa transmembrane domain and a 482 aa cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGFR3 (Flt-4). VEGFR3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGFR3 may be important for lymph angiogenesis.

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Long Name
Vascular Endothelial Growth Factor Receptor 3
Entrez Gene IDs
2324 (Human); 14257 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; FLT4; Flt-4; fms-related tyrosine kinase 4; LMPH1A; PCLFLT41; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; Tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3; VEGF R3; VEGFR3; VEGFR-3; VEGFR3Fms-like tyrosine kinase 4

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Citations for Human VEGFR3/Flt-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Characterizing Lymphangiogenesis and Concurrent Inflammation in Adipose Tissue in Response to VEGF-D
    Authors: A Chakrabort, CK Scogin, K Rizwan, TS Morley, JM Rutkowski
    Front Physiol, 2020-04-22;11(0):363.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Correlation of Vascular Endothelial Growth Factor subtypes and their receptors with melanoma progression: A next-generation Tissue Microarray (ngTMA) automated analysis
    Authors: SM Seyed Jafa, C Wiedmer, S Cazzaniga, Ž Frangež, M Shafighi, H Beltramine, B Weber, HU Simon, RE Hunger
    PLoS ONE, 2018-11-08;13(11):e0207019.
    Species: Human
    Sample Types: Tissue Microarray
    Applications: IHC-P
  3. Heparins that block VEGF-A-mediated von Willebrand factor fiber generation are potent inhibitors of hematogenous but not lymphatic metastasis
    Authors: L Goertz, SW Schneider, A Desch, FT Mayer, J Koett, K Nowak, I Karampinis, MK Bohlmann, V Umansky, AT Bauer
    Oncotarget, 2016-10-18;7(42):68527-68545.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Further evidence for expression and function of the VEGF-C/VEGFR-3 axis in cancer cells.
    Authors: Su JL, Chen PS, Chien MH, Chen PB, Chen YH, Lai CC, Hung MC, Kuo ML
    Cancer Cell, 2008-06-01;13(6):557-60.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007-04-30;177(3):489-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. The VEGF-C/Flt-4 axis promotes invasion and metastasis of cancer cells.
    Authors: Su JL, Yang PC, Shih JY, Yang CY, Wei LH, Hsieh CY, Chou CH, Jeng YM, Wang MY, Chang KJ, Hung MC, Kuo ML
    Cancer Cell, 2006-03-01;9(3):209-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines
    Authors: Ling Xu, Martin Hausmann, Wolfgang Dietmaier, Silvia Kellermeier, Theresa Pesch, Manuela Stieber-Gunckel et al.
    BMC Cancer

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Human VEGFR3/Flt-4 Antibody
By Anonymous on 11/02/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Kidney tissue Species: Human
Immunocytochemistry/Immunofluorescence Human VEGFR3/Flt-4 Antibody MAB3491