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Human VEGF-C Biotinylated Antibody

Catalog #: BAF752
2 Citations Datasheet
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BAF752
Product Details
Citations (2)
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Human VEGF-C Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGF-C in Western blots. In this format, less than 1% cross‑reactivity is observed with rhVEGF121 and rhVEGF165b.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
recombinant human VEGF-C
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human VEGF-C (Catalog # 2179-VC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF-C

Vascular endothelial growth factor C (VEGF-C) and VEGF-D constitute a VEGF sub-family that share the conserved VEGF homology domain (VHD) with other VEGF family members but are distinguished by their preferential formation of non-covalent homodimers. Both VEGF-C and -D have long N- and C-terminal propeptide extensions. The VEGF-C propeptide undergoes stepwise proteolytic processing to generate ligands with increasing affinity for VEGF-R3. However, only the fully processed VEGF-C containing just the VHD can bind VEGF-R2. None of the VEGF-C forms have appreciable affinity for VEGF-R1. VEGF-C is expressed in multiple adult human tissues, most prominently in lymph nodes, heart, placenta, ovary, and small intestine. Traces of VEGF-C are also detected in brain, liver, thymus, skeletal muscles, spleen, prostate, testis and colon. Unlike other VEGF family members, VEGF-C expression is not regulated by hypoxia. VEGF-C is a lymphangiogenic growth factor and the VEGF-C/VEGF-R3 signaling pathway has been shown to be crucial for lymphangiogenesis. VEGF-C and VEGF-R3 are usually co-expressed at sites with lymphatic vessel sprouting, in the embryo, and in various pathological conditions. VEGF-C stimulates lymphangiogenesis in the avian chorioallantoic membrane model. Over-expression of VEGF-C in breast cancer cells has been shown to increase intratumoral lymphangiogenesis, resulting in enhanced metastasis to regional lymph nodes and to the lungs. Mouse tumors expressing elevated levels of VEGF-C have increased lymphatic metastasis and increased lymphatic surface area in the tumor margin. VEGF-C is also associated with lymph node metastasis of colorectal carcinoma. Besides lymphangiogenesis, VEGF-C can have potent effects on physiological angiogenesis through its interaction with VEGF R2. The protein can stimulate migration and proliferation of endothelial cells in vitro and in vivo and has been shown to stimulate angiogenesis in the mouse cornea and in rabbit hind limb ischaemia (1 - 10).

References
  1. Joukov, V. et al. (1996) EMBO J. 15:290.
  2. Joukov, V. et al. (1997) EMBO J. 16:3898.
  3. Lee, J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:1988.
  4. Oh, S. et al. (1997) Dev. Biol. 188:96.
  5. Witzenbichler, B. et al. (1998) Am. J. Pathol. 153:381.
  6. Cao, Y. et al. (1998) Proc. Natl. Acad. Sci. 95:14389.
  7. Stacker, S and M. Achen (1999) Growth Factors 17:1.
  8. Akagi, K. et al. (2000) Br. J. Cancer 83:887.
  9. Skobe, M. et al. (2001) Nature Medicine 2:192.
  10. Padera, T.P. et al. (2002) Science 296:1883.
Long Name
Vascular Endothelial Growth Factor C
Entrez Gene IDs
7424 (Human); 22341 (Mouse)
Alternate Names
Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM

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Citations for Human VEGF-C Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Ccbe1 regulates Vegfc-mediated induction of Vegfr3 signaling during embryonic lymphangiogenesis.
    Authors: Le Guen L, Karpanen T, Schulte D, Harris N, Koltowska K, Roukens G, Bower N, van Impel A, Stacker S, Achen M, Schulte-Merker S, Hogan B
    Development, 2014-02-12;141(6):1239-49.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  2. Regulation of lymphatic capillary regeneration by interstitial flow in skin.
    Authors: Goldman J, Conley KA, Raehl A, Bondy DM, Pytowski B, Swartz MA, Rutkowski JM, Jaroch DB, Ongstad EL
    Am. J. Physiol. Heart Circ. Physiol., 2006-12-22;292(5):H2176-83.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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