Home / VCAM-1/CD106 / Human VCAM-1/CD106 PE-conjugated Antibody

Human VCAM-1/CD106 PE-conjugated Antibody

Catalog #: FAB5649P
9 Citations Datasheet
Catalog # Availability Size / Price Qty
FAB5649P
Detection of VCAM‑1/CD106 in HuT 78 Human Cell Line by Flow Cytometry.
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Citations (9)
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Human VCAM-1/CD106 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.
Source
Monoclonal Mouse IgG2A Clone # IE10
Purification
Protein A or G purified from ascites
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VCAM-1/CD106 antibody in HuT 78 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of VCAM‑1/CD106 in HuT 78 Human Cell Line by Flow Cytometry. HuT 78 human cutaneous T cell lymphoma cell line was stained with Mouse Anti-Human VCAM-1/CD106 PE-conjugated Monoclonal Antibody (Catalog # FAB5649P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VCAM-1/CD106

Vascular cell adhesion molecule 1 (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.

Long Name
Vascular Cell Adhesion Molecule 1
Entrez Gene IDs
7412 (Human); 22329 (Mouse)
Alternate Names
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

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Citations for Human VCAM-1/CD106 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. DC8 and DC13 var Genes Associated with Severe Malaria Bind Avidly to Diverse Endothelial Cells
    Authors: Marion Avril, Andrew J. Brazier, Martin Melcher, Sowmya Sampath, Joseph D. Smith
    PLoS Pathogens
  2. ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells
    Authors: D Eckert, F Rapp, AT Tsedeke, J Molendowsk, R Lehn, M Langhans, C Fournier, F Rödel, S Hehlgans
    Cells, 2021-12-27;11(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Differences in Endothelial Activation and Dysfunction Induced by Antiphospholipid Antibodies Among Groups of Patients With Thrombotic, Refractory, and Non-refractory Antiphospholipid Syndrome
    Authors: M Velásquez, LF Peláez, M Rojas, R Narváez-Sá, JA Velásquez, C Escudero, S San Martín, ÁP Cadavid
    Frontiers in Physiology, 2021-12-02;12(0):764702.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The FGF, TGF&beta and WNT axis Modulate Self-renewal of Human SIX2+ Urine Derived Renal Progenitor Cells
    Authors: MS Rahman, W Wruck, LS Spitzhorn, L Nguyen, M Bohndorf, S Martins, F Asar, A Ncube, L Erichsen, N Graffmann, J Adjaye
    Sci Rep, 2020-01-20;10(1):739.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruption
    Authors: M Avril, M Benjamin, MM Dols, JD Smith
    Sci Rep, 2019-09-11;9(1):13142.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Effects of gravitational mechanical unloading in endothelial cells: association between caveolins, inflammation and adhesion molecules.
    Authors: Grenon S, Jeanne M, Aguado-Zuniga J, Conte M, Hughes-Fulford M
    Sci Rep, 2013-01-01;3(0):1494.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Characterization of renal progenitors committed toward tubular lineage and their regenerative potential in renal tubular injury.
    Authors: Angelotti ML, Ronconi E, Ballerini L, Peired A, Mazzinghi B, Sagrinati C, Parente E, Gacci M, Carini M, Rotondi M, Fogo AB, Lazzeri E, Lasagni L, Romagnani P
    Stem Cells, 2012-08-01;30(8):1714-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  8. Expression, Regulation, and Function of Atypical Chemerin Receptor CCRL2 on Endothelial Cells.
    Authors: Monnier J, Lewen S, O'Hara E, Huang K, Tu H, Butcher EC, Zabel BA
    J. Immunol., 2012-06-13;189(2):956-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Protein C activation peptide inhibits the expression of ICAM-1, VCAM-1, and interleukin-8 induced by TNF-a in human dermal microvascular endothelial cells
    Authors: María Del Socorro Pina-Canseco, Araceli Páez-Arenas, Felipe Massó, Eduardo Pérez-Campos, Ruth Martínez-Cruz, Pedro Hernández-Cruz et al.
    Folia Histochemica et Cytobiologica

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