Human VCAM-1/CD106 Antibody

Catalog #: MAB8092 Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8092-100
MAB8092-SP

Detection of Human VCAM‑1/CD106 by Western Blot.

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All VCAM-1/CD106 Antibodies

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Human VCAM-1/CD106 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VCAM-1/CD106 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2691A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese Hamster Ovary cell line CHO-derived human VCAM-1/CD106
Phe25-Glu698
Accession # P19320-1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

4 µg/mL

HUVEC human umbilical vein endothelial cells treated Recombinant Human TNF‑ alpha (Catalog # 210-TA)

Flow Cytometry

0.25 µg/10⁶ cells

Hut78 human cutaneous T cell lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human VCAM‑1/CD106 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 10 ng/mL Recombinant Human TNF‑ alpha (210-TA) for 24 hours. PVDF membrane was probed with 4 µg/mL of Rabbit Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB8092) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for VCAM‑1/CD106 at approximately 110 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry

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Detection of VCAM-1/CD106 in Hut78 Human Cell Line by Flow Cytometry. Hut78 human cutaneous T cell lymphoma cell line was stained with Rabbit Anti-Human VCAM-1/CD106 Monoclonal Antibody (Catalog # MAB8092, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110). Staining was performed using our Staining Membrane-associated Proteins protocol.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

VCAM-1, also known as CD106, is an immunoglobulin (Ig)-like adhesion molecule that is mainly expressed in endothelial cells and other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes (1, 2). It plays a critical role in inflammation by recruiting leukocytes to acute and chronic inflammation sites (3, 4). Alternatively-spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 aa extracellular domain (ECD) that includes seven C2-type immunoglobulin domains, a 22 aa transmembrane segment, and a 19 amino acid (aa) cytoplasmic tail. Within the ECD, human VCAM-1 shares 75% and 76% aa sequence identity with the mouse and rat VCAM-1, respectively. VCAM-1 binds to leukocyte integrins alpha 4 beta 1 (VLA-4) and alpha 4 beta 7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. The VCAM-1:VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation (5). ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals, but elevated levels of serum VCAM-1 are indicative of future Atrial Fibrillation incident as well as liver disease (6, 7). Tumor cells use overexpression of VCAM-1 as means of escaping immune surveillance (8).

References

Vonderheide, R.H. et al. (1994) J. Cell Biol. 125:215.
Cybulsky, M.I. et al. (1991) Proc. Natl. Acad. Sci. USA 88:7859.
Luster, A.D. et al. (2005) Nat. Immunol. 6:1182.
Osborn, L. et al. (1989) Cell 59:1203
Langer. H.F. et al. 2009. J Cell Mol Med. 13:1211.
Willeit.K. et al. 2017. JAMA Cardiol. 2:516.
Lo Iacono.O. et al. 2008. Liver Int. 28:1129.
Wu.T.C. et al. 2007. Cancer Research. 67:6003

Long Name

Vascular Cell Adhesion Molecule 1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Alternate Names

CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1