Human VCAM-1/CD106 Antibody

Catalog #: MAB11671 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB11671-100
MAB11671-SP

Detection of VCAM1 in Human Tonsil via seqIF™ staining on COMET™

2 Images

All VCAM-1/CD106 Antibodies

Product Details

FAQs

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VCAM-1/CD106 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human VCAM-1 in Direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1027406

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human VCAM-1/CD106
Phe25-Glu698
Accession # P19320

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human tonsil

Multiplex Immunofluorescence

15 µg/mL

Immersion fixed paraffin-embedded sections of human tonsil

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Multiplex Immunofluorescence

View Larger

Detection of VCAM1 in Human Tonsil via seqIF™ staining on COMET™ VCAM1 was detected in immersion fixed paraffin-embedded sections of human Tonsil using Mouse Anti-Human VCAM1, Monoclonal Antibody (Catalog #MAB11671) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Immunohistochemistry

View Larger

Detection of VCAM‑1/CD106 in Human Tonsil. VCAM‑1/CD106 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB11671) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

VCAM-1, also known as CD106, is an immunoglobulin (Ig)-like adhesion molecule that is mainly expressed in endothelial cells and other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes (1, 2). It plays a critical role in inflammation by recruiting leukocytes to acute and chronic inflammation sites (3, 4). Alternatively-spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 aa extracellular domain (ECD) that includes seven C2-type immunoglobulin domains, a 22 aa transmembrane segment, and a 19 amino acid (aa) cytoplasmic tail. Within the ECD, human VCAM-1 shares 75% and 76% aa sequence identity with the mouse and rat VCAM-1, respectively. VCAM-1 binds to leukocyte integrins alpha 4 beta 1 (VLA-4) and alpha 4 beta 7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. The VCAM-1:VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation (5). ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals, but elevated levels of serum VCAM-1 are indicative of future Atrial Fibrillation incident as well as liver disease (6, 7). Tumor cells use overexpression of VCAM-1 as means of escaping immune surveillance (8).

References

Vonderheide, R.H. et al. (1994) J. Cell Biol. 125:215.
Cybulsky, M.I. et al. (1991) Proc. Natl. Acad. Sci. USA 88:7859.
Luster, A.D. et al. (2005) Nat. Immunol. 6:1182.
Osborn, L. et al. (1989) Cell 59:1203
Langer. H.F. et al. 2009. J Cell Mol Med. 13:1211.
Willeit.K. et al. 2017. JAMA Cardiol. 2:516.
Lo Iacono.O. et al. 2008. Liver Int. 28:1129.
Wu.T.C. et al. 2007. Cancer Research. 67:6003

Long Name

Vascular Cell Adhesion Molecule 1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Alternate Names

CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1