Human Tyro3/Dtk Antibody

Detection of Human Tyro3/Dtk by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, U-87 MG human glioblastoma/astrocytoma cell line, and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Tyro3/Dtk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF859) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Tyro3/Dtk at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Dtk/TYRO3 by Western Blot rVSV∆G/EBOV entry kinetics: (A) VeroS cells were infected with rVSV∆G/EBOV at multiple MOIs, inoculum was removed after 1 h, and monitored for luciferase production over time. (B) VeroS, HEK293T, and HAP1 cells were infected with rVSV∆G/EBOV (MOI 1), inoculum was not removed, and monitored for luciferase production over time. Right panel zooms in to display the signals observed in HAP1 and HEK293T cells. (C) Immunoblots demonstrating production of the transfected receptors in HEK293T cells. (D) HEK293T cells transfected with the indicated receptors were infected with rVSV∆G/EBOV and monitored for luciferase production over time. Each experiment was repeated in duplicate, three independent times. Data shown are the averages of averages and corresponding SEM of three independent trials. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33348746), licensed under a CC-BY license. Not internally tested by R&D Systems.