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Human TSLPR Antibody

Catalog #: AF981
8 Citations Datasheet
Catalog # Availability Size / Price Qty
AF981
AF981-SP
Detection of Human TSLP R by Western Blot.
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Citations (8)
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Human TSLPR Antibody Summary

Species Reactivity
Human
Specificity
Detects human TSLP R in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TSLP R
Val30-Phe232
Accession # Q9HC73
Formulation
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.100 EU per 1 µg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2-1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
Immersion fixed THP‑1 human acute monocytic leukemia cell line treated with PMA
Neutralization
Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑7 R alpha and TSLP R. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 0.3 ng/mL Recombinant Human TSLP.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TSLP R antibody by Western Blot. View Larger

Detection of Human TSLP R by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human TSLP R. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TSLP R at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human TSLP R antibody by Western Blot. View Larger

Detection of Human TSLP R by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TSLP R at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry TSLP R antibody in Human Lymph Node by Immunohistochemistry (IHC-P). View Larger

TSLP R in Human Lymph Node. TSLP R was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to the plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry View Larger

TSLPR in THP‑1 Human Cell Line. TSLPR was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line treated with PMA (positive staining) and K562 human chronic myelogenous leukemia cell line (negative staining) using Goat Anti-Human TSLPR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Cell Proliferation Induced by TSLP and Neutralization by Human TSLP R Antibody. View Larger

Cell Proliferation Induced by TSLP and Neutralization by Human TSLP R Antibody. Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-7 Ra and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.3 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981). The ND50 is typically 1-5 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: TSLPR

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS‑like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3-6). The TSLP R and IL-7 R alpha are coexpressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell‑attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (5).

References
  1. Fujio, K. et al. (2000) Blood 95:2204.
  2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
  3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  4. Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
  5. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  6. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Long Name
Thymic Stromal Lymphopoietin Receptor
Entrez Gene IDs
64109 (Human); 57914 (Mouse); 171499 (Rat)
Alternate Names
CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP R; TSLP receptor; TSLPR

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Citations for Human TSLPR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells
    Authors: SZ Li, XX Jin, XL Ge, YG Zuo, HZ Jin
    J Immunol Res, 2020-09-24;2020(0):4594630.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Combinatorial IL-17RB, ST2, and TSLPR Signaling in Dendritic Cells of Patients With Allergic Rhinitis
    Authors: Rui Zheng, Yang Chen, Jianbo Shi, Kai Wang, Xuekun Huang, Yueqi Sun et al.
    Frontiers in Cell and Developmental Biology
  3. Detection of Minimal Bone Marrow involvement of Blastic Plasmacytoid Dendritic Cell Neoplastic Cells - CD303 immunostaining as a diagnostic tool
    Authors: R Ohe, NY Aung, Y Shiono, A Utsunomiya, T Kabasawa, N Tamazawa, Y Tamura, T Kato, A Yamada, S Hasegawa, K Aizawa, K Inokura, S Ito, T Toubai, Y Kato, T Tsunoda, K Onami, T Suzuki, K Ishizawa, M Yamakawa
    J Clin Exp Hematop, 2018-02-08;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Pr
  4. TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils
    Authors: B Zhang, CY Wei, KK Chang, JJ Yu, WJ Zhou, HL Yang, J Shao, JJ Yu, MQ Li, F Xie
    Oncol Lett, 2017-10-03;14(6):7483-7488.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Thymic stromal lymphopoietin-induced HOTAIR activation promotes endothelial cell proliferation and migration in atherosclerosis
    Authors: Y Peng, K Meng, L Jiang, Y Zhong, Y Yang, Y Lan, Q Zeng, L Cheng
    Biosci. Rep., 2017-07-12;37(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. CRLF2 over-expression is a poor prognostic marker in children with high risk T-cell acute lymphoblastic leukemia
    Authors: Chiara Palmi, Angela M. Savino, Daniela Silvestri, Ilaria Bronzini, Gunnar Cario, Maddalena Paganin et al.
    Oncotarget
  7. IL-3 and TNF alpha increase Thymic Stromal Lymphopoietin Receptor (TSLPR) expression on eosinophils and enhance TSLP-stimulated degranulation
    Authors: Ellen B Cook, James L Stahl, Elizabeth A Schwantes, Kristen E Fox, Sameer K Mathur
    Clinical and Molecular Allergy
  8. Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
    Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
    Blood, 2010-06-10;116(12):2061-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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