Human TSLPR Antibody Summary
Gly25-Lys231
Accession # Q9HC73
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TSLP R by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line mock transfected or transfected with human TSLP R. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human TSLP R Monoclonal Antibody (Catalog # MAB9811) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for TSLP R at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of TSLPR in NS0 cells transfected with hTSLPR vs Irrelevant NS0 transfectant cells by Flow Cytometry. NS0 cells transfected with hTSLPR (filled histogram) vs Irrelevant NS0 transfectant cells (open histogram) were stained with Mouse Anti-Human TSLPR Monoclonal Antibody (Catalog # MAB9811) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSLPR
TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS-like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3‑6). The TSLP R and IL-7 R alpha are co‑expressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (5).
- Fujio, K. et al. (2000) Blood 95:2204.
- Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
- Park, L.S. et al. (2000) J. Exp. Med. 192:659.
- Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
- Reche, P.A. et al. (2001) J. Immunol. 167:336.
- Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Product Datasheets
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