Human TSLPR Antibody Summary
Gly25-Lys231
Accession # Q9HC73
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TSLPR in HEK cells by Flow Cytometry. HEK cells transfected with hTSLPR (filled histogram) vs Irrelevant HEK transfectant cells (open histogram) were stained with Mouse Anti-Human TSLPR Monoclonal Antibody (Catalog # MAB981), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSLPR
TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS-like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3‑6). The TSLP R and IL-7 R alpha are co‑expressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (5).
- Fujio, K. et al. (2000) Blood 95:2204.
- Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
- Park, L.S. et al. (2000) J. Exp. Med. 192:659.
- Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
- Reche, P.A. et al. (2001) J. Immunol. 167:336.
- Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Product Datasheets
Citations for Human TSLPR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Thymic stromal chemokine TSLP acts through Th2 cytokine production to induce cutaneous T cell lymphoma
Cancer Res, 2016-09-06;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
DeltaNp63 controls a TLR3-mediated mechanism that abundantly provides thymic stromal lymphopoietin in atopic dermatitis.
Authors: Kubo T, Kamekura R, Kumagai A, Kawata K, Yamashita K, Mitsuhashi Y, Kojima T, Sugimoto K, Yoneta A, Sumikawa Y, Yamashita T, Sato N, Himi T, Ichimiya S
PLoS ONE, 2014-08-29;9(8):e105498.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Thymic stromal lymphopoietin receptor-mediated IL-6 and CC/CXC chemokines expression in human airway smooth muscle cells: role of MAPKs (ERK1/2, p38, and JNK) and STAT3 pathways.
Authors: Shan L, Redhu NS, Saleh A
J. Immunol., 2010-05-14;184(12):7134-43.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Thymic stromal lymphopoietin-induced human asthmatic airway epithelial cell proliferation through an IL-13-dependent pathway.
Authors: Semlali A, Jacques E, Koussih L, Gounni AS, Chakir J
J. Allergy Clin. Immunol., 2010-03-17;125(4):844-50.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: ICC, IHC, Western Blot
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