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Human TrkA Antibody

Catalog #: AF175
15 Citations Datasheet
Catalog # Availability Size / Price Qty
AF175
AF175-SP
Detection of Human TrkA by Western Blot.
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Product Details
Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TrkA Antibody Summary

Species Reactivity
Human
Specificity
Detects human TrkA in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat TrkA is observed and 10% cross-reactivity with recombinant human (rh) TrkB and rhTrKC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TrkA
Ala33-Glu407
Accession # AAA36770
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
K562 human chronic myelogenous leukemia cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 5 ng/mL Recombinant Human beta ‑NGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TrkA antibody by Western Blot. View Larger

Detection of Human TrkA by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF175) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TrkA at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry TrkA antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

TrkA in Human Colon. TrkA was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF175) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in colon glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by beta ‑NGF and Neutralization by Human TrkA Antibody. View Larger

Cell Proliferation Induced by beta ‑NGF and Neutralization by Human TrkA Antibody. Recombinant Human beta -NGF (Catalog # 256-GF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta -NGF (5 ng/mL) is neutralized (green line) by increasing concent-rations of Goat Anti-Human TrkA Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF175). The ND50 is typically 3-12 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkA

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB and TrkC preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily. NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when expressed independent of Trk.

References
  1. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
  2. Sofroniew, M.V. et al. (200) Annu. Rev. Neurosci. 24:1217.
Long Name
Neurotrophic Tyrosine Kinase Receptor A
Entrez Gene IDs
4914 (Human); 18211 (Mouse); 59109 (Rat)
Alternate Names
DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A

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Citations for Human TrkA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. In vivo survival and differentiation of Friedreich ataxia iPSC-derived sensory neurons transplanted in the adult dorsal root ganglia
    Authors: Viventi S, Frausin S, Howden SE et al.
    Stem cells translational medicine
  2. Generation of self-organized autonomic ganglion organoids from fibroblasts
    Authors: Liu S, Xiang K, Yuan F, Xiang M
    iScience
  3. Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons
    Authors: AJ Hulme, JR McArthur, S Maksour, S Miellet, L Ooi, DJ Adams, RK Finol-Urda, M Dottori
    Frontiers in Cellular Neuroscience, 2020-12-04;14(0):600895.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Nerve growth factor receptor role on rabbit sperm storage
    Authors: C Castellini, S Mattioli, AD Bosco, E Cotozzolo, A Cartoni Ma, M Rende, AM Stabile, A Pistilli
    Theriogenology, 2020-05-11;153(0):54-61.
    Species: Rabbit
    Sample Types: Whole Cells, Whole Cells
    Applications: Flow Cytometry, Neutralization
  5. In vitro effect of nerve growth factor on the main traits of rabbit sperm
    Authors: C Castellini, S Mattioli, A Dal Bosco, G Collodel, A Pistilli, AM Stabile, L Macchioni, F Mancuso, G Luca, M Rende
    Reprod. Biol. Endocrinol., 2019-11-12;17(1):93.
    Species: Rabbit
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, ICC, Neutralization, Western Blot
  6. Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA
    Authors: S Desiderio, S Vermeiren, C Van Campen, S Kricha, E Malki, S Richts, EV Fletcher, T Vanwelden, BZ Schmidt, KA Henningfel, T Pieler, CG Woods, V Nagy, C Verfaillie, EJ Bellefroid
    Cell Rep, 2019-03-26;26(13):3522-3536.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells
    Authors: AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori
    Sci Rep, 2018-01-12;8(1):603.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. TrkA is a binding partner of NPM‐ALK that promotes the survival of ALK + T‐cell lymphoma
    Authors: Wenyu Shi, Suraj Konnath George, Bhawana George, Choladda V. Curry, Albina Murzabdillaeva, Serhan Alkan et al.
    Molecular Oncology
  9. TrkB-containing exosomes promote the transfer of glioblastoma aggressiveness to YKL-40-inactivated glioblastoma cells
    Authors: Sandra Pinet, Barbara Bessette, Nicolas Vedrenne, Aurélie Lacroix, Laurence Richard, Marie-Odile Jauberteau et al.
    Oncotarget
  10. Nerve growth factor downregulates inflammatory response in human monocytes through TrkA.
    Authors: Prencipe G, Minnone G, Strippoli R, De Pasquale L, Petrini S, Caiello I, Manni L, De Benedetti F, Bracci-Laudiero L
    J Immunol, 2014-02-28;192(7):3345-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection.
    Authors: Flowerdew S, Wick D, Himmelein S, Horn A, Sinicina I, Strupp M, Brandt T, Theil D, Hufner K
    PLoS ONE, 2013-12-19;8(12):e83603.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Age-related regional differences in cardiac nerve growth factor expression
    Authors: Erol Saygili, Rahel Kluttig, Obaida R. Rana, Esra Saygili, Christopher Gemein, Matthias D. Zink et al.
    Age (Dordr)
  13. TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
    Authors: Huang YT, Lai PC, Wu CC
    Int. J. Oncol., 2010-10-01;37(4):943-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  14. BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
    Authors: Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
    Int. J. Oncol., 2010-06-01;36(6):1469-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Neurotrophin signaling through tropomyosin receptor kinases contributes to survival and proliferation of non-Hodgkin lymphoma.
    Authors: Sniderhan LF, Garcia-Bates TM, Burgart M, Bernstein SH, Phipps RP, Maggirwar SB
    Exp. Hematol., 2009-08-28;37(11):1295-309.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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