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Human TREM-1 Biotinylated Antibody

Catalog #: BAF1278
2 Citations Datasheet
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BAF1278
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TREM-1 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TREM‑1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse (rm) TREM-1 and rmTREM-2b is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TREM-1
Met1-Arg200
Accession # Q9NP99
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TREM‑1 Fc Chimera (Catalog # 1278-TR)
Flow Cytometry
2.5 µg/106 cells
Human whole blood monocytes and neutrophils

Human TREM-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human TREM‑1 Antibody (Catalog # AF1278)

Standard: Recombinant Human TREM-1 Fc Chimera Protein, CF (Catalog # 1278-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS‑induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS‑induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).

References
  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs
54210 (Human); 58217 (Mouse)
Alternate Names
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1

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Citations for Human TREM-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Soluble Triggering Receptor Expressed on Myeloid Cells 1 in lung cancer
    Authors: A Kuemmel, A Alflen, LH Schmidt, M Sebastian, R Wiewrodt, AB Schulze, R Buhl, M Radsak
    Sci Rep, 2018-07-17;8(1):10766.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  2. Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
    Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM
    Chest, 2007-05-15;132(1):58-63.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development

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