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Human TREM-1 Antibody

Catalog #: AF1278
8 Citations Datasheet
Catalog # Availability Size / Price Qty
AF1278
AF1278-SP
Detection of Human TREM‑1 by Western Blot.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TREM-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TREM-1 in ELISAs and Western blots. In ELISAs, less than 0.2% cross-reactivity with recombinant mouse (rm) TREM‑1 and rmTREM‑2b is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TREM-1
Met1-Arg200
Accession # Q9NP99
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
Human whole blood monocytes and neutrophils
Agonist Activity
Measured by its ability to stimulate TNF-alpha secretion by human peripheral blood mononuclear cells. Bouchon, A. et al. (2001) Nature 410:1103 and Bouchon, A. et al. (2000) J. Immunology 164:4991.
The ED50 for this effect is typically 2 - 6 μg/mL.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human TREM-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human TREM‑1 Biotinylated Antibody (Catalog # BAF1278)

Standard: Recombinant Human TREM-1 Fc Chimera Protein, CF (Catalog # 1278-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TREM-1 antibody by Western Blot. View Larger

Detection of Human TREM‑1 by Western Blot. Western blot shows lysates of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TREM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1278) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TREM-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with
LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).

References
  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs
54210 (Human); 58217 (Mouse)
Alternate Names
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1

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Citations for Human TREM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Novel potent liposome agonists of triggering receptor expressed on myeloid cells 2 phenocopy antibody treatment in cells
    Authors: Christophe Boudesco, Annelies Nonneman, Alessandro Cinti, Paola Picardi, Loredana Redaelli, Sofie Swijsen et al.
    Glia
  2. Single-cell RNA sequencing identifies an Il1rn+/Trem1+ macrophage subpopulation as a cellular target for mitigating the progression of thoracic aortic aneurysm and dissection
    Authors: X Liu, W Chen, G Zhu, H Yang, W Li, M Luo, C Shu, Z Zhou
    Cell Discovery, 2022-02-08;8(1):11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Bulk and Single-Cell Profiling of Breast Tumors Identifies TREM-1 as a Dominant Immune Suppressive Marker Associated With Poor Outcomes
    Authors: Ashok K. Pullikuth, Eric D. Routh, Kip D. Zimmerman, Julia Chifman, Jeff W. Chou, Michael H. Soike et al.
    Frontiers in Oncology
  4. TREM-1 orchestrates angiotensin II–induced monocyte trafficking and promotes experimental abdominal aortic aneurysm
    Authors: Marie Vandestienne, Yujiao Zhang, Icia Santos-Zas, Rida Al-Rifai, Jeremie Joffre, Andreas Giraud et al.
    Journal of Clinical Investigation
  5. Inhibition of TREM‐1 attenuates inflammation and lipid accumulation in diet‐induced nonalcoholic fatty liver disease
    Authors: Shenzong Rao, Jingsong Huang, Zhijun Shen, Changgang Xiang, Min Zhang, Xueliang Lu
    Journal of Cellular Biochemistry
  6. Identification of a novel splice variant isoform of TREM-1 in human neutrophil granules1
    Authors: Sankar Baruah, Kathy Keck, Michelle Vrenios, Marshall R. Pope, Merideth Pearl, Kevin Doerschug et al.
    The Journal of Immunology
  7. TREM-1 Mediates Inflammatory Injury and Cardiac Remodeling Following Myocardial Infarction
    Authors: Amir Boufenzer, Jérémie Lemarié, Tabassome Simon, Marc Derive, Youcef Bouazza, Nguyen Tran et al.
    Circulation Research
  8. Metalloproteinases shed TREM-1 ectodomain from lipopolysaccharide-stimulated human monocytes.
    Authors: Gomez-Pina V, Soares-Schanoski A, Rodriguez-Rojas A, Del Fresno C, Garcia F, Vallejo-Cremades MT, Fernandez-Ruiz I, Arnalich F, Fuentes-Prior P, Lopez-Collazo E
    J. Immunol., 2007-09-15;179(6):4065-73.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, ICC, Western Blot

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