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Human TREM-1 Antibody

Catalog #: MAB1278
19 Citations Datasheet
Clone 193015 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB1278-SP
MAB1278-500
MAB1278-100
Detection of TREM‑1 in Human Blood Granulocytes by Flow Cytometry.
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Citations (19)
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Human TREM-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TREM-1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 193015
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TREM-1
Ala21-Asn205
Accession # Q9NP99
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Agonist Activity
Measured by its ability to stimulate TNF-alpha secretion by human peripheral blood mononuclear cells [Bouchon, A. et al. (2001) Nature 410:1103 and Bouchon, A. et al. (2000) J. Immunology 164:4991].
The ED50 for this effect is typically 1-4 μg/mL.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TREM-1 antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of TREM‑1 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human TREM-1 Monoclonal Antibody (Catalog # MAB1278, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with
LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).

References
  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs
54210 (Human); 58217 (Mouse)
Alternate Names
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1

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Citations for Human TREM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. HIV and HCV augments inflammatory responses through increased TREM-1 expression and signaling in Kupffer and Myeloid cells
    Authors: J Hyun, RS McMahon, AL Lang, JS Edwards, AD Badilla, ME Greene, GW Stone, S Pallikkuth, M Stevenson, DM Dykxhoorn, S Kottilil, S Pahwa, E Thomas
    PLoS Pathog., 2019-07-01;15(7):e1007883.
  2. Triggering receptor expressed on myeloid cells-1 deletion in mice attenuates high-fat diet-induced obesity
    Authors: Benjamin Brustolin, Nina Touly, Marine Maillefer, Lola Parisot, Elisa Di Pillo, Marc Derive et al.
    Front Endocrinol (Lausanne)
  3. Novel potent liposome agonists of triggering receptor expressed on myeloid cells 2 phenocopy antibody treatment in cells
    Authors: Christophe Boudesco, Annelies Nonneman, Alessandro Cinti, Paola Picardi, Loredana Redaelli, Sofie Swijsen et al.
    Glia
  4. Potentiation of NETs release is novel characteristic of TREM-1 activation and the pharmacological inhibition of TREM-1 could prevent from the deleterious consequences of NETs release in sepsis
    Authors: Amir Boufenzer, Kevin Carrasco, Lucie Jolly, Benjamin Brustolin, Elisa Di-Pillo, Marc Derive et al.
    Cellular & Molecular Immunology
  5. TREM-1 Protects HIV-1-Infected Macrophages from Apoptosis through Maintenance of Mitochondrial Function
    Authors: GR Campbell, RK To, SA Spector
    MBio, 2019-11-12;10(6):.
    Species: Human
    Sample Types: Whole Blood
    Applications: Functional Assay
  6. Enriched Cd141+ DCs in the joint are transcriptionally distinct, activated, and contribute to joint pathogenesis
    Authors: M Canavan, AM Walsh, V Bhargava, SM Wade, T McGarry, V Marzaioli, B Moran, M Biniecka, H Convery, S Wade, C Orr, R Mullan, JM Fletcher, S Nagpal, DJ Veale, U Fearon
    JCI Insight, 2018-12-06;3(23):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  7. Targeted endothelial gene deletion of Triggering Receptor Expressed on Myeloid cells-1 protects mice during septic shock
    Authors: L Jolly, K Carrasco, M Derive, J Lemarié, A Boufenzer, S Gibot
    Cardiovasc. Res., 2018-05-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Activation
  8. Regulation of Human Macrophage M1–M2 Polarization Balance by Hypoxia and the Triggering Receptor Expressed on Myeloid Cells-1
    Authors: Federica Raggi, Simone Pelassa, Daniele Pierobon, Federica Penco, Marco Gattorno, Francesco Novelli et al.
    Frontiers in Immunology
  9. TREM-1 SNP rs2234246 regulates TREM-1 protein and mRNA levels and is associated with plasma levels of L-selectin
    Authors: AA Aldasoro A, S Dadé, M Stathopoul, M Derive, N Coumba Ndi, T Xie, C Masson, S Gibot, S Visvikis-S
    PLoS ONE, 2017-08-03;12(8):e0182226.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  10. TREM-1 and its potential ligands in non-infectious diseases: from biology to clinical perspectives
    Authors: A Tammaro, M Derive, S Gibot, JC Leemans, S Florquin, MC Dessing
    Pharmacol. Ther, 2017-02-27;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  11. TREM-1 links dyslipidemia to inflammation and lipid deposition in atherosclerosis
    Authors: Daniel Zysset, Benjamin Weber, Silvia Rihs, Jennifer Brasseit, Stefan Freigang, Carsten Riether et al.
    Nature Communications
  12. TREM-1low is a novel characteristic for tumor-associated macrophages in lung cancer
    Oncotarget, 2016-06-28;7(26):40508-40517.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Cutting Edge: identification of neutrophil PGLYRP1 as a ligand for TREM-1.
    Authors: Read C, Kuijper J, Hjorth S, Heipel M, Tang X, Fleetwood A, Dantzler J, Grell S, Kastrup J, Wang C, Brandt C, Hansen A, Wagtmann N, Xu W, Stennicke V
    J Immunol, 2015-01-16;194(4):1417-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker in vitro and in vivo.
    Authors: Bosco MC, Pierobon D, Blengio F, Raggi F, Vanni C, Gattorno M, Eva A, Novelli F, Cappello P, Giovarelli M, Varesio L
    Blood, 2010-12-10;117(9):2625-39.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Comparison of gene expression profiles between human and mouse monocyte subsets.
    Authors: Ingersoll MA, Spanbroek R, Lottaz C
    Blood, 2009-11-12;115(3):e10-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Intervention of an inflammation amplifier, triggering receptor expressed on myeloid cells 1, for treatment of autoimmune arthritis.
    Authors: Murakami Y, Akahoshi T, Aoki N, Toyomoto M, Miyasaka N, Kohsaka H
    Arthritis Rheum., 2009-06-01;60(6):1615-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  17. TREM-1 expression in tumor-associated macrophages and clinical outcome in lung cancer.
    Authors: Ho CC, Liao WY, Wang CY, Lu YH, Huang HY, Chen HY, Chan WK, Chen HW, Yang PC
    Am. J. Respir. Crit. Care Med., 2007-12-20;177(7):763-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
    Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM
    Chest, 2007-05-15;132(1):58-63.
    Species: Human
    Sample Types: BALF, Exhaled Ventilator Condensate (EVC)
    Applications: Dot Blot, ELISA Development
  19. Effects of TREM-1 activation in human neutrophils: activation of signaling pathways, recruitment into lipid rafts and association with TLR4.
    Authors: Fortin CF, Lesur O, Fulop T
    Int. Immunol., 2006-11-10;19(1):41-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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