Human Transgelin/TAGLN Alexa Fluor® 532-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Transgelin/TAGLN

TAGLN (Transgelin; also 22 kDa Actin-binding protein, WS3-10 and Smooth muscle protein 22 alpha) is a 22-24 kDa cytosolic member of the calponin (calcium-binding and calmodulin-binding troponin T-like protein) family of molecules. It is expressed in both visceral and vascular smooth muscle, fibroblasts, cardiac myocytes, and potentially in breast duct plus prostate epithelium. TAGLN is associated with the actin stress fibers and appears to both suppress MMP-9 production, and downmodulate Ca⁺⁺-independent smooth muscle contraction. Human TAGLN is 201 amino acids (aa) in length. It contains one CH/calponin homology domain (aa 24-137), and an actin-binding calponin-like repeat/CLIK (aa 175-200). There is one utilized phosphorylation site at Tyr193, plus two utilized acetylation sites in the N-terminus. Three potential isoform variants are reported, one that shows a alternative start site at Met111, a second that contains a new start site 10 aa upstream of the standard site, and a third that possesses a 58 aa substitution for aa 97-201. Full-length human and mouse TAGLN share 97% aa sequence identity.

Product Datasheets

Related Research Areas

• Cancer Biomarkers
• Cardiac Stem Cell Markers
• Contractile Mechanism and Cytoskeleton in VSMC
• Extracellular Matrix Remodeling and Cell Migration During EMT
• Myogenesis Markers
• Smooth Muscle Cell Markers