Human TRAIL R2/TNFRSF10B Antibody

Catalog #: MAB6313 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
MAB6313
MAB6313-SP

Detection of Human TRAIL R2/TNFRSF10B by Western Blot.

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All TRAIL R2/TNFRSF10B Antibodies

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Human TRAIL R2/TNFRSF10B Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRAIL R2/TNFRSF10B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhOPG, rhRANK, rhRELT, rhTNF RI, rhTNF RII, rhTRAIL R1, rhTRAIL R3, rhTRAIL R4, or rhTWEAK R is observed. Additionally in direct ELISAs, no cross‑reactivity with rhTRAIL R4, rhXEDAR, recombinant mouse (rm) DR5, rhTRAIL R1, rmTRAIL R2, or rmTNFRH3 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 603307

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TRAIL R2/TNFRSF10B
Ile56-Pro128
Accession # NP_003833

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TRAIL R2/TNFRSF10B by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TRAIL R2/TNFRSF10B Monoclonal Antibody (Catalog # MAB6313) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for TRAIL R2/TNFRSF10B at approximately 44 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also known as DR5 and TRICK 2, is a type 1 membrane protein of the TNF R family, designated TNFRSF10B, which is a receptor for TRAIL (APO2 ligand). The human TRAIL R2 cDNA encodes a 440 amino acid (aa) precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% aa sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

References

Chaudhary, P.M. et al. (1997) Immunity 7:821.
Walczak, H. et al. (1997) EMBO J. 16:5386.
Golstein, P. (1997) Curr. Biol. 7:R750.

Long Name

TRAIL Receptor 2

Entrez Gene IDs

8795 (Human); 21933 (Mouse)

Alternate Names

CD262 antigen; CD262; death domain containing receptor for TRAIL/Apo-2L; Death receptor 5; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; KILLERapoptosis inducing protein TRICK2A/2B; TNF-related apoptosis-inducing ligand receptor 2; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRAIL-R2apoptosis inducing receptor TRAIL-R2; TRAILR2cytotoxic TRAIL receptor-2; TRICK2; TRICK2A; TRICKB; tumor necrosis factor receptor superfamily member 10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor-like protein ZTNFR9; ZTNFR9