Human TRAF-6 Antibody

Catalog #: AF3284
4 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
AF3284
AF3284-SP

Detection of Human TRAF‑6 by Western Blot.

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All TRAF-6 Antibodies

Product Details

Citations (4)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRAF-6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRAF-6 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-1, -2, -3, -4, and -5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TRAF-6
Met1-Val522
Accession # Q9Y4K3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

HEK293T human embryonic kidney cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TRAF‑6 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TRAF-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3284) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, -TRAF5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-6 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western

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Detection of Human TRAF‑6 by Simple Western^TM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for TRAF‑6 at approximately 63 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TRAF‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3284). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAF-6

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL‑1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs2-6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-6 is a 522 amino acid, 60 kDa protein. Overexpression of TRAF-6 mediates the activation of NF-kappa-B and JNK signaling pathways. TRAF-3 and TRAF-6 have been identified as adaptor molecules involved in TLR/IL-1R signaling events.

Long Name

TNF Receptor-Associated Factor 6

Entrez Gene IDs

7189 (Human); 22034 (Mouse)

Alternate Names

E3 ubiquitin-protein ligase TRAF6; EC 6.3.2.-; Interleukin-1 signal transducer; RING finger protein 85; RNF85MGC:3310; TNF receptor-associated factor 6; TRAF6; TRAF-6

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human TRAF-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Pyruvate kinase M2 mediates IL-17 signaling in keratinocytes driving psoriatic skin inflammation
Authors: FP Veras, GA Publio, BM Melo, DS Prado, T Norbiato, NT Cecilio, C Hiroki, LEA Damasceno, R Jung, JE Toller-Kaw, TV Martins, SF Assunção, D Lima, MG Alves, GV Vieira, LA Tavares, ALR Alves-Reze, SH Karbach, HI Nakaya, TM Cunha, CS Souza, FQ Cunha, KU Sales, A Waisman, JC Alves-Filh
Cell Reports, 2022-12-27;41(13):111897.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
The deubiquitinase Usp27x as a novel regulator of cFLIPL protein expression and sensitizer to death-receptor-induced apoptosis
Authors: Manuel Nico Dold, Xiulin Ng, Claudia Alber, Ian Edward Gentle, Georg Häcker, Arnim Weber
Apoptosis
Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA
Authors: YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li
Chin. Med. J., 2020-03-20;0(6):679-686.
Species: Human
Sample Types: Cell Lysate
Applications: Western Blot
USP17 Suppresses Tumorigenesis and Tumor Growth through Deubiquitinating AEP
Authors: X Chen, C Wang, K Liao, S Zhou, L Cao, J Chen, C Xu, Y Lin
Int. J. Biol. Sci., 2019-01-29;15(4):738-748.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot