Home / TRA-1-60(R) / Human TRA-1-60(R) PE-conjugated Antibody

Human TRA-1-60(R) PE-conjugated Antibody

Catalog #: FAB4770P
3 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
FAB4770P
Detection of TRA‑1‑60(R) in BG01V Human Cells by Flow Cytometry.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRA-1-60(R) PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRA‑1‑60(R).
Source
Monoclonal Mouse IgM Clone # TRA-1-60
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
2102Ep human embryonal carcinoma cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TRA-1-60(R) antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of TRA‑1‑60(R) in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60(R) PE-conjugated Monoclonal Antibody (Catalog # FAB4770P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRA-1-60(R)

TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2-4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).

References
  1. Andrews, P. et al. (1984) Hybridoma 3:347.
  2. Thomson, J. et al. (1998) Science 282:1145.
  3. Giwercman, A. et al. (1993) Cancer 72:1308.
  4. Marrink, J. et al. (1991) Int. J. Cancer 49:368.
  5. Badcock, G. et al. (1999) Cancer Res. 59:4715.
  6. Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
Long Name
Tumor-related Antigen-1-60 [R]
Entrez Gene IDs
5420 (Human)
Alternate Names
GCTM-2 antigen; GP135; gp200; PC; PCLP; PCLP-1; PDX; Podocalyxin; Podocalyxin-like protein 1; PODXL1; TRA160(R); TRA-1-60(R)

Product Datasheets

You must select a language.

x
Product Datasheet

Citations for Human TRA-1-60(R) PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors
    Authors: Breanna S. Borys, Tiffany Dang, Tania So, Leili Rohani, Tamas Revay, Tylor Walsh et al.
    Stem Cell Research & Therapy
  2. Optimized serial expansion of human induced pluripotent stem cells using low-density inoculation to generate clinically relevant quantities in vertical-wheel bioreactors
    Authors: Breanna S. Borys, Tania So, James Colter, Tiffany Dang, Erin L. Roberts, Tamas Revay et al.
    Stem Cells Translational Medicine
  3. Chemically Defined and Small Molecule-Based Generation of Human Cardiomyocytes
    Authors: Paul W. Burridge, Elena Matsa, Praveen Shukla, Ziliang C. Lin, Jared M. Churko, Antje D. Ebert et al.
    Nature Methods

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human TRA-1-60(R) PE-conjugated Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human TRA-1-60(R) PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human TRA-1-60(R) PE-conjugated Antibody
By Anonymous on 12/07/2016
Application: Flow Sample Tested: Breast cancer cells Species: Human
Flow Cytometry Human TRA-1-60(R) PE-conjugated Antibody FAB4770P