Human Total ER alpha/NR3A1 DuoSet IC ELISA

Catalog #: DYC5715-2
2 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
DYC5715-5
DYC5715-2

Ancillary Products Available

DY999B: Substrate Reagent Pack

DY999: Substrate Reagent Pack

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All ER alpha/NR3A1 ELISAs

Product Details

Citations (2)

FAQs

Supplemental Products

Reviews (1)

Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Total ER alpha/NR3A1 DuoSet IC ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4 hours 40 mins (after plate preparation)

Sample Volume Required

Cell lysates (100 µL)

Assay Range

15.6 - 1,000 pg/mL

Sufficient Materials

Kits available for two, five, or fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total ER alpha / NR3A1 in cell lysates. An immobilized capture antibody specific for ER alpha / NR3A1 binds both phosphorylated and unphosphorylated ER alpha / NR3A1. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Available in 2, 5, and 15- (96-well) plate pack sizes
Economical alternative to Western blot

Kit Content

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂O₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

ELISA

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Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ER alpha/NR3A1

Estrogen receptor alpha (ER alpha, NR3A1) is a member of the steroid receptor family. The natural ligand for ER is the classic estrogenic compound 17 beta-estradiol. ER alpha is expressed in a wide variety of tissues such as bones, uterus, bladder, ovary, prostate, testis, epididymis, kidney, breast, heart, vessel wall, pituitary and hypothalamus. The estrogen receptor has a variety of central physiological roles, including those involved in maintenance of the reproductive, cardiovascular, musculoskeletal and central nervous systems. ER alpha has been shown to form homodimers as well as heterodimers with ER beta. Both ER alpha and ER beta can give rise to numerous isoforms.

Long Name:

Estrogen Receptor alpha

Entrez Gene IDs:

2099 (Human)

Alternate Names:

ER alpha; ER; Era; ER-alpha; ESR1; ESRESRA; Estradiol receptor; estrogen receptor 1; estrogen receptor alpha delta 3*4,56,7*/819-2 isoform; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha delta 4*5,6,7*/654 isoform; estrogen receptor alpha; estrogen receptor; NR3A1; NR3A1DKFZp686N23123; Nuclear receptor subfamily 3 group A member 1

Citations for Human Total ER alpha/NR3A1 DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Diptoindonesin G is a middle domain HSP90 modulator for cancer treatment
Authors: K Donahue, H Xie, M Li, A Gao, M Ma, Y Wang, R Tipton, N Semanik, T Primeau, S Li, L Li, W Tang, W Xu
The Journal of Biological Chemistry, 2022-11-14;0(0):102700.
Species: Human
Sample Types: Cell Lysates
Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers
Authors: José M Pingarrón
Biosensors (Basel), 2016-11-21;6(4):.
Species: Human
Sample Types: Cell Lysates