Human Total Bad DuoSet IC ELISA

Catalog #: DYC819-2 Datasheet

Discontinued Product

DYC819-2 has been discontinued.

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Summary Product Features Kit Content Other Reagents Required Product Datasheets Preparation and Storage Background

Human Total Bad DuoSet IC ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4 hours 40 mins (after plate preparation)

Sample Volume Required

Cell lysates (100 µL)

Assay Range

125.0 - 8,000 pg/mL

Sufficient Materials

Kits available for two, five, or fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure in cell lysates. An immobilized capture antibody specific for binds both phosphorylated and unphosphorylated . After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Available in 2, 5, and 15- (96-well) plate pack sizes
Economical alternative to Western blot

Kit Content

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂O₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Bad

The Bcl-2 family of proteins consists of both inhibitors and promoters of apoptosis. The relative ratios of pro- and anti-apoptotic Bcl-2 family proteins determine the sensitivity or resistance of cells to a variety of apoptotic signals. The pro-apoptoptic Bad (Bcl-2-antagonist of cell death) heterodimerizes strongly with Bcl-x, less strongly with Bcl-2, and not at all with Bax, Bcl-xS, Mcl-1, A1, or itself. When Bad dimerizes with Bcl-xL, Bax is displaced and its apoptosis-inducing ability is restored.

Long Name:

Bcl-xL/Bcl-2 Associated Death Promoter

Entrez Gene IDs:

572 (Human); 12015 (Mouse); 64639 (Rat)

Alternate Names:

Bad; BBC2; BBC6; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; Bcl-2-binding component 6; BCL2-binding component 6; BCL2-binding protein; BCL2L8; Bcl2-L-8; BCL2L8bcl2-L-8; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-XL/Bcl-2-associated death promoter