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Human TNF RI/TNFRSF1A Antibody

Catalog #: AB-225-PB
3 Citations Datasheet
Catalog # Availability Size / Price Qty
AB-225-PB
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TNF RI/TNFRSF1A Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TNF RII, rhTNF-alpha, recombinant mouse TNF-alpha, recombinant rat TNF-alpha, and rhTNF-beta is observed. Because this antibody preparation is a total IgG fraction, complete monospecificity cannot be assumed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human sTNF RI/TNFRSF1A
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)
Agonist Activity
Measured in a cytotoxicity assay using either L-929 mouse fibrosarcoma cells or A549 human lung carcinoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 10-15 μg/mL for L-929 cells or 5-10 μg/mL for A549 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily member, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-  alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1 - 3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6 - 8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Human TNF RI is a 455 amino acid (aa) protein that contains a 21 aa signal sequence, a 190 aa ECD with a PLAD domain(5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 221 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of human TNF RI shows 80%, 80%, 73%, 69% and 70% aa identity with dog, cat, pig, rat and mouse TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII.

References
  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  4. Banner, D.W et al. (1993) Cell 73: 431.
  5. Chan, F.K. et al. (2000) Science 288:2351.
  6. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  7. Jin, L. et al. (2000) J. Immunol. 165:5153.
  8. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  9. Legler, D.F. et al. (2003) Immunity 18:655.
  10. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  11. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  12. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  13. McDermott, M.F. et al. (1999) Cell 97:133.
  14. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
  15. Schall, T.J. et al. (1990) Cell 61:361.
Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

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Citations for Human TNF RI/TNFRSF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Small molecules that inhibit TNF signalling by stabilising an asymmetric form of the trimer
    Authors: J O'Connell, J Porter, B Kroeplien, T Norman, S Rapecki, R Davis, D McMillan, T Arakaki, A Burgin, D Fox Iii, T Ceska, F Lecomte, A Maloney, A Vugler, B Carrington, BP Cossins, T Bourne, A Lawson
    Nat Commun, 2019-12-19;10(1):5795.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. CAR T Cells Targeting MISIIR for the Treatment of Ovarian Cancer and Other Gynecologic Malignancies
    Authors: A Rodriguez-, P Sharma, M Poussin, AC Boesteanu, NG Minutolo, SB Gitto, DK Omran, MK Robinson, GP Adams, F Simpkins, DJ Powell
    Mol. Ther., 2019-12-06;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  3. A class of small molecules that inhibit TNFalpha-induced survival and death pathways via prevention of interactions between TNFalphaRI, TRADD, and RIP1.
    Authors: Gururaja TL, Yung S, Ding R, Huang J, Zhou X, McLaughlin J, Daniel-Issakani S, Singh R, Cooper RD, Payan DG, Masuda ES, Kinoshita T
    Chem. Biol., 2007-10-01;14(10):1105-18.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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