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Human TNF RI/TNFRSF1A Antibody

Catalog #: MAB225
42 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB225-SP
MAB225-100
MAB225-500
TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody.
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Citations (42)
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Human TNF RI/TNFRSF1A Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF RI in Western blots. In Western blots, does not cross-react with recombinant human (rh) TNF RII, rmTNF RI, or rmTNF RII.
Source
Monoclonal Mouse IgG1 Clone # 16803
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TNF RI/TNFRSF1A
Accession # NP_001056
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)
under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize TNF RI/TNFRSF1A-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.008-0.04 µg/mL in the presence of 15 ng/mL Recombinant Human TNF RI/TNFRSF1A, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody. View Larger

TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody. Recombinant Human TNF RI/TNFRSF1A (Catalog # 372-R1) inhibits Recombinant Human TNF-a (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225). The ND50 is typically 0.008-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Flow Cytometry Detection of TNF RI/TNFRSF1A antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Immunohistochemistry Detection of Human TNF RI/TNFRSF1A by Immunohistochemistry View Larger

Detection of Human TNF RI/TNFRSF1A by Immunohistochemistry Representative immunohistochemical staining for TNFR1 and TNFR2 in the kidneys.(A) Images were captured at 200× (a, b, c, d, i, j, k, l) and 100× (e, f, g, h, m, n, o, p) magnification. Images show the negative controls in the glomeruli (a, i) and tubulointerstitium (e, m) and TNFR1 and TNFR2 immunostaining in the glomeruli (b, j) and tubulointerstitium (f, n) of the normal kidneys, respectively. TNFR1 and TNFR2 immunostaining is shown in the glomeruli (c, k) and tubulointerstitium (g, o) of the kidneys from selected IgAN patients who had levels of serum TNFR2 that ranked in the lowest 10 [low group (LG)]. TNFR1 and TNFR2 immunostaining in the glomeruli (d, l) and tubulointerstitium (h, p) of the kidneys from selected IgAN patients who had levels of serum TNFR2 that ranked in the highest 10 [high group (HG)], respectively, are also shown. (B) Percentage of the TNFR1 and TNFR2-positive area in the kidneys were evaluated. Glomerular and tubulointerstitial TNFR expression was elevated significantly in IgAN patients compared with those in control (Ctrl) subjects. The tubulointerstitial TNFR2-positive area was significantly larger in HG than in LG. However, there was no significant difference in the tubulointerstitial TNFR1 and glomerular TNFR areas between LG and HG. * P < 0.01, **P < 0.001, †P < 0.0001. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0122212), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

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Citations for Human TNF RI/TNFRSF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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  1. NFATc2 is an intrinsic regulator of melanoma dedifferentiation.
    Authors: V Perotti, P Baldassari, A Molla et al.
    Oncogene
  2. Pro-inflammatory TNF-alpha and IFN-gamma Promote Tumor Growth and Metastasis via Induction of MACC1
    Authors: Dennis Kobelt, Chenyu Zhang, Isabelle Ailish Clayton-Lucey, Rainer Glauben, Cynthia Voss, Britta Siegmund et al.
    Frontiers in Immunology
  3. An integrated organoid omics map extends modeling potential of kidney disease
    Authors: Lassé, M;El Saghir, J;Berthier, CC;Eddy, S;Fischer, M;Laufer, SD;Kylies, D;Hutzfeldt, A;Bonin, LL;Dumoulin, B;Menon, R;Vega-Warner, V;Eichinger, F;Alakwaa, F;Fermin, D;Billing, AM;Minakawa, A;McCown, PJ;Rose, MP;Godfrey, B;Meister, E;Wiech, T;Noriega, M;Chrysopoulou, M;Brandts, P;Ju, W;Reinhard, L;Hoxha, E;Grahammer, F;Lindenmeyer, MT;Huber, TB;Schlüter, H;Thiel, S;Mariani, LH;Puelles, VG;Braun, F;Kretzler, M;Demir, F;Harder, JL;Rinschen, MM;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  4. Dynamic bioinspired coculture model for probing ER+ breast cancer dormancy in the bone marrow niche
    Authors: L Pradhan, D Moore, EM Ovadia, SL Swedzinski, T Cossette, RA Sikes, K van Golen, AM Kloxin
    Science Advances, 2023-03-08;9(10):eade3186.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. TNF-alpha Affects Signature Cytokines of Th1 and Th17 T Cell Subsets through Differential Actions on TNFR1 and TNFR2
    Authors: B Pesce, CH Ribeiro, M Larrondo, V Ramos, L Soto, D Catalán, JC Aguillón
    International Journal of Molecular Sciences, 2022-08-18;23(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Plasmacytoid dendritic cells have divergent effects on HIV infection of initial target cells and induce a pro-retention phenotype
    Authors: O Tong, G Duette, TR O'Neil, CM Royle, H Rana, B Johnson, N Popovic, S Dervish, MAE Brouwer, H Baharlou, E Patrick, G Ctercteko, S Palmer, E Lee, E Hunter, AN Harman, AL Cunningham, N Nasr
    PloS Pathogens, 2021-04-19;17(4):e1009522.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. A class of viral inducer of degradation of the necroptosis adaptor RIPK3 regulates virus-induced inflammation
    Authors: Zhijun Liu, Himani Nailwal, Jonah Rector, Masmudur M. Rahman, Richard Sam, Grant McFadden et al.
    Immunity
  8. A conformation-selective monoclonal antibody against a small molecule-stabilised signalling-deficient form of TNF
    Authors: DJ Lightwood, RJ Munro, J Porter, D McMillan, B Carrington, A Turner, A Scott-Tuck, ES Hickford, A Schmidt, D Fox, A Maloney, T Ceska, T Bourne, J O'Connell, ADG Lawson
    Nature Communications, 2021-01-25;12(1):583.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Androgens predispose males to monocyte-mediated immunopathology by inducing the expression of leukocyte recruitment factor CXCL1
    Authors: J Sellau, M Groneberg, H Fehling, T Thye, S Hoenow, C Marggraff, M Weskamm, C Hansen, S Stanelle-B, S Kuehl, J Noll, V Wolf, NG Metwally, SH Hagen, C Dorn, J Wernecke, H Ittrich, E Tannich, T Jacobs, I Bruchhaus, M Altfeld, H Lotter
    Nat Commun, 2020-07-10;11(1):3459.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  10. The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-alpha/IL-33/ST2L signaling promotes gastric cancer metastasis
    Authors: Q Zhou, X Wu, X Wang, Z Yu, T Pan, Z Li, X Chang, Z Jin, J Li, Z Zhu, B Liu, L Su
    Oncogene, 2019-10-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  11. Intrinsic tumor necrosis factor-alpha pathway is activated in a subset of patients with focal segmental glomerulosclerosis
    Authors: Chen-Fang Chung, Thomas Kitzler, Nadezda Kachurina, Katarina Pessina, Sima Babayeva, Martin Bitzan et al.
    PLOS ONE
  12. Human TNF-Luc reporter mouse: A new model to quantify inflammatory responses
    Authors: F Minshawi, MRH White, W Muller, N Humphreys, D Jackson, BJ Campbell, A Adamson, S Papoutsopo
    Sci Rep, 2019-01-17;9(1):193.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Low doses of LPS exacerbate the inflammatory response and trigger death on TLR3-primed human monocytes
    Authors: M Monguió-To, M Franquesa, MR Sarrias, FE Borràs
    Cell Death Dis, 2018-05-01;9(5):499.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor
    Authors: AT Holdbrooks, CM Britain, SL Bellis
    J. Biol. Chem., 2017-12-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage
    Authors: A Chakrabort, KA Dorsett, HQ Trummell, ES Yang, PG Oliver, JA Bonner, DJ Buchsbaum, SL Bellis
    J. Biol. Chem., 2017-11-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Bispecific T cell engager (BiTE�) antibody constructs can mediate bystander tumor cell killing
    Authors: SL Ross, M Sherman, PL McElroy, JA Lofgren, G Moody, PA Baeuerle, A Coxon, T Arvedson
    PLoS ONE, 2017-08-24;12(8):e0183390.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Neutrophil Necroptosis Is Triggered by Ligation of Adhesion Molecules following GM-CSF Priming
    Authors: Xiaoliang Wang, Zhaoyue He, He Liu, Shida Yousefi, Hans-Uwe Simon
    The Journal of Immunology
  18. TNF? promotes CAR-dependent migration of leukocytes across epithelial monolayers
    Sci Rep, 2016-05-19;6(0):26321.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Novel unbiased assay for circulating podocyte-toxic factors associated with recurrent focal segmental glomerulosclerosis
    Authors: Nadezda Kachurina, Chen-Fang Chung, Erin Benderoff, Sima Babayeva, Martin Bitzan, Paul Goodyer et al.
    American Journal of Physiology-Renal Physiology
  20. Tumor necrosis factor receptor 2-signaling in CD133-expressing cells in renal clear cell carcinoma
    Authors: Rafia S Al-Lamki, Jun Wang, Jun Yang, Natalie Burrows, Patrick H Maxwell, Timothy Eisen et al.
    Oncotarget
  21. Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF-alpha and the NF-kappa B pathway
    Authors: Christina Katanov, Shalom Lerrer, Yulia Liubomirski, Leonor Leider-Trejo, Tsipi Meshel, Jair Bar et al.
    Stem Cell Research & Therapy
  22. Targeting autocrine HB-EGF signaling with specific ADAM12 inhibition using recombinant ADAM12 prodomain
    Authors: Miles A. Miller, Marcia L. Moss, Gary Powell, Robert Petrovich, Lori Edwards, Aaron S. Meyer et al.
    Scientific Reports
  23. Circulating TNF receptors 1 and 2 are associated with the severity of renal interstitial fibrosis in IgA nephropathy.
    Authors: Sonoda Y, Gohda T, Suzuki Y, Omote K, Ishizaka M, Matsuoka J, Tomino Y
    PLoS ONE, 2015-04-10;10(4):e0122212.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  24. Negative selection by apoptosis enriches progenitors in naive and expanded human umbilical cord blood grafts.
    Authors: Mizrahi K, Ash S, Peled T, Yaniv I, Stein J, Askenasy N
    Bone Marrow Transplant, 2014-04-28;49(7):942-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Apoptotic signaling through Fas and TNF receptors ameliorates GVHD in mobilized peripheral blood grafts.
    Authors: Mizrahi K, Yaniv I, Ash S, Stein J, Askenasy N
    Bone Marrow Transplant, 2014-02-24;49(5):640-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. High efficiency cell-specific targeting of cytokine activity.
    Authors: Garcin G, Paul F, Staufenbiel M, Bordat Y, Van der Heyden J, Wilmes S, Cartron G, Apparailly F, De Koker S, Piehler J, Tavernier J, Uze G
    Nat Commun, 2014-01-01;5(0):3016.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Tumor necrosis factor stimulates osteoclastogenesis from human bone marrow cells under hypoxic conditions.
    Authors: Nomura T, Aoyama M, Waguri-Nagaya Y, Goto Y, Suzuki M, Miyazawa K, Asai K, Goto S
    Exp Cell Res, 2013-12-17;321(2):167-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Enhancement of TWIK-related acid-sensitive potassium channel 3 (TASK3) two-pore domain potassium channel activity by tumor necrosis factor alpha.
    Authors: El Hachmane M, Rees K, Veale E, Sumbayev V, Mathie A
    J Biol Chem, 2013-12-04;289(3):1388-401.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  29. The Receptor AXL Diversifies EGFR Signaling and Limits the Response to EGFR-Targeted Inhibitors in Triple-Negative Breast Cancer Cells
    Authors: Aaron S. Meyer, Miles A. Miller, Frank B. Gertler, Douglas A. Lauffenburger
    Science Signaling
  30. Erythropoietin Protects Adult Retinal Ganglion Cells against NMDA-, Trophic Factor Withdrawal-, and TNF-alpha -Induced Damage
    Authors: Zhi-Yang Chang, Ming-Kung Yeh, Chiao-Hsi Chiang, Yi-Hao Chen, Da-Wen Lu
    PLoS ONE
  31. Mislocalization of death receptors correlates with cellular resistance to their cognate ligands in human breast cancer cells
    Authors: Jun-Jie Chen, H.-C. Jennifer Shen, Leslie A. Rivera Rosado, Yaqin Zhang, Xu Di, Baolin Zhang
    Oncotarget
  32. The transmembrane domains of TNF-related apoptosis-inducing ligand (TRAIL) receptors 1 and 2 co-regulate apoptotic signaling capacity.
    Authors: Neumann S, Bidon T, Branschadel M, Krippner-Heidenreich A, Scheurich P, Doszczak M
    PLoS ONE, 2012-08-03;7(8):e42526.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. The latency-associated UL138 gene product of human cytomegalovirus sensitizes cells to tumor necrosis factor alpha (TNF-alpha) signaling by upregulating TNF-alpha receptor 1 cell surface expression
    Authors: Christina Montag, Jutta Annabella Wagner, Iris Gruska, Barbara Vetter, Lüder Wiebusch, Christian Hagemeier
    Journal of Virology
  34. Neuronal PINCH is Regulated by TNF-alpha and is Required for Neurite Extension
    Authors: Asavari Jatiani, Paola Pannizzo, Elisa Gualco, Luis Del-Valle, Dianne Langford
    Journal of Neuroimmune Pharmacology
  35. Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
    Authors: Renaud SJ, Sullivan R, Graham CH
    Placenta, 2009-02-08;30(4):313-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  36. Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
    Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
    J. Allergy Clin. Immunol., 2008-12-05;123(1):239-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. Differential responses of FLIPLong and FLIPShort-overexpressing human myeloid leukemia cells to TNF-alpha and TRAIL-initiated apoptotic signals.
    Authors: Seal S, Hockenbery DM, Spaulding EY, Kiem HP, Abbassi N, Deeg HJ
    Exp. Hematol., 2008-10-05;36(12):1660-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  38. IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
    Authors: Kolliputi N, Waxman AB
    Am. J. Respir. Cell Mol. Biol., 2008-09-05;40(3):314-24.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  39. Death receptor expression is associated with poor response to chemotherapy and shorter survival in metastatic ovarian carcinoma.
    Authors: Dong HP, Kleinberg L, Silins I, Florenes VA, Trope CG, Risberg B, Nesland JM, Davidson B
    Cancer, 2008-01-01;112(1):84-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  40. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006-08-17;241(1):7-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  41. A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation.
    Authors: Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK
    Cell Death Differ., 2006-01-20;13(10):1789-801.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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