Human TNF-alpha (Research Grade Adalimumab Biosimilar) Biotinylated Antibody

Catalog #: IC9677B Datasheet

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
IC9677B-100

Detection of TNF‑ alpha in Human PBMCs by Flow Cytometry.

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All TNF-alpha Antibodies

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Human TNF-alpha (Research Grade Adalimumab Biosimilar) Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TNF- alpha in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Adalimumab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu7

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human TNF‑ alpha

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Biotin

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of TNF- alpha antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of TNF‑ alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (A) treated with PMA and Ca2+ ionomycin overnight or (B) resting, were stained with Human Anti-Human TNF- alpha (Research Grade Adalimumab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9677B) followed by APC-conjugated Streptavidin (Catalog # F0050) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TNF-alpha

Adalimumab is a biosimilar directed against the extracellular domain of TNF- alpha. The biotinylated antibody can be used in flow cytometry or various assay format to measure TNF- alpha expression and can be detected by conjugated streptavidin.

Long Name

Tumor Necrosis Factor alpha

Entrez Gene IDs

7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)

Alternate Names

APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha