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Human TNF-alpha QuantiGlo ELISA Kit

Catalog #: QTA00C
25 Citations Datasheet
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QTA00C
Control Products Available
QC196: QuantiGlo Immunoassay Control Set 732 for Human TNF-alpha
HIV-1 Gag p24 Standard Curve
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Product Details
Citations (25)
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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human TNF-alpha QuantiGlo ELISA Kit Summary

Assay Length
5.0 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), Plasma (100 uL)
Sensitivity
0.481 pg/mL
Assay Range
2.2 - 7,000 pg/mL
Specificity
Natural and recombinant human TNF-alpha.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The QuantiGlo Human TNF-alpha Immunoassay is a 5 hour solid phase chemiluminescent ELISA designed to measure human TNF-alpha in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human TNF-alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TNF-alpha. Results obtained using naturally occurring TNF-alpha samples showed linear curves that were parallel to the standard curves obtained using the QuantiGlo® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TNF-alpha.

Recovery

The recovery of human TNF-alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 104 98-100
EDTA Plasma (n=4) 96 90-104
Heparin Plasma (n=4) 95 88-100
Serum (n=4) 91 87-97

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human TNF-alpha in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
HIV-1 Gag p24 Linearity
Human TNF- alpha Linearity
Human IL-2 ELISA Linearity
Human TNF-alpha Chemiluminescent ELISA Linearity

Scientific Data

N/A HIV-1 Gag p24 Standard Curve View Larger

HIV-1 Gag p24 Standard Curve

N/A Human TNF-alpha Chemiluminescent ELISA Calibrator Diluent RD5P (1X) Standard Curve View Larger

Human TNF-alpha Chemiluminescent ELISA Calibrator Diluent RD5P (1X) Standard Curve

N/A Human IL-2 ELISA Cell Culture Supernate Standard Curve View Larger

Human IL-2 ELISA Cell Culture Supernate Standard Curve

N/A Human TNF- alpha Standard Curve View Larger

Human TNF- alpha Standard Curve

N/A Human IL-2 ELISA Serum/Plasma Standard Curve View Larger

Human IL-2 ELISA Serum/Plasma Standard Curve

N/A Human TNF-alpha Chemiluminescent ELISA Calibrator Diluent RD6N Standard Curve View Larger

Human TNF-alpha Chemiluminescent ELISA Calibrator Diluent RD6N Standard Curve

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Product Datasheet

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TNF-alpha

Tumor Necrosis Factor alpha (TNF-alpha ), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily (1). It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (2-5). TNF-alpha is also involved in a number of pathological conditions including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmunity, and cancer (5-11). 

 Human TNF-alpha is synthesized as a 26 kDa type II transmembrane protein that consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (12, 13). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus monkey, and 71%-92% aa identity with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. It is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (14). Cell surface TNF-alpha can both induce the lysis of tumor cells and virus infected cells, and generate its own downstream cell signaling following ligation by soluble TNF RI (15, 16). Shedding of membrane bound TNF-alpha by TACE/ ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (17-19). 
 TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (20, 21) and the hematopoietic cell-restricted 78-80 kDa TNF RII (22, 23), both of which are also expressed as homotrimers (1, 24). Both type I and type II receptors bind TNF-alpha with comparable affinity and can promote NF kappa B activation (25-28). Only TNF RI, however, contains a cytoplasmic death domain which triggers the activation of apoptosis (3, 29). Soluble forms of both types of receptors are released into human serum and urine and can neutralize the biological activity of TNF-alpha (30-32).

Long Name:
Tumor Necrosis Factor alpha
Entrez Gene IDs:
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names:
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

Citations for Human TNF-alpha QuantiGlo ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Intestinal Dysbiosis, Tight Junction Proteins, and Inflammation in Rheumatoid Arthritis Patients: A Cross-Sectional Study
    Authors: Mucientes, A;Lisbona-Montañez, JM;Mena-Vázquez, N;Ruiz-Limón, P;Manrique-Arija, S;García-Studer, A;Ortiz-Márquez, F;Fernández-Nebro, A;
    International journal of molecular sciences
    Species: Human
    Sample Types: Plasma
  2. Controlled adsorption of multiple bioactive proteins enables targeted mast cell nanotherapy
    Authors: Du, F;Rische, CH;Li, Y;Vincent, MP;Krier-Burris, RA;Qian, Y;Yuk, SA;Almunif, S;Bochner, BS;Qiao, B;Scott, EA;
    Nature nanotechnology
    Species: Human
    Sample Types: Adenoid Lavage Fluid
  3. A randomized phase 2 study on demeclocycline in patients with mild-to-moderate COVID-19
    Authors: Iwahori, K;Nii, T;Yamaguchi, N;Kawasaki, T;Okamura, S;Hashimoto, K;Matsuki, T;Tsujino, K;Miki, K;Osa, A;Goya, S;Abe, K;Mori, M;Takeda, Y;Yamada, T;Kida, H;Kumanogoh, A;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  4. Bioactive multi-protein adsorption enables targeted mast cell nanotherapy
    Authors: F Du, C Rische, Y Li, M Vincent, R Krier-Burr, Y Qian, S Yuk, S Almunif, B Bochner, B Qiao, E Scott
    Research square, 2023-01-26;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Motoric Cognitive Risk Syndrome, Subtypes and 8-Year All-Cause Mortality in Aging Phenotypes: The Salus in Apulia Study
    Authors: I Bortone, R Zupo, F Castellana, S Aresta, L Lampignano, S Sciarra, C Griseta, TA Stallone, G Sborgia, M Lozupone, F Panza, G Lagravines, P Battista, R Sardone
    Brain sciences, 2022-06-29;12(7):.
    Species: Human
    Sample Types: Serum
  6. Liver Fibrosis and 8-Year All-Cause Mortality Trajectories in the Aging Cohort of the Salus in Apulia Study
    Authors: R Zupo, F Castellana, S De Nucci, G De Pergola, M Lozupone, I Bortone, M Castellana, G Sborgia, L Lampignano, G Giannelli, F Panza, R Sardone
    Biomedicines, 2021-11-04;9(11):.
    Species: Human
    Sample Types: Serum
  7. Hydrogen gas inhalation alleviates oxidative stress in patients with post-cardiac arrest syndrome
    Authors: T Tamura, M Suzuki, K Hayashida, Y Kobayashi, J Yoshizawa, T Shibusawa, M Sano, S Hori, J Sasaki
    J Clin Biochem Nutr, 2020-04-03;67(2):214-221.
    Species: Human
    Sample Types: Plasma
  8. Association between Fetal Adipokines and Child Behavioral Problems at Preschool Age: The Hokkaido Study on Environment and Children's Health
    Authors: M Minatoya, S Itoh, A Araki, N Tamura, K Yamazaki, C Miyashita, R Kishi
    Int J Environ Res Public Health, 2018-01-11;15(1):.
    Species: Human
    Sample Types: Whole Blood
  9. Impact of nutritional indices on mortality in patients with heart failure
    Authors: A Yoshihisa, Y Kanno, S Watanabe, T Yokokawa, S Abe, M Miyata, T Sato, S Suzuki, M Oikawa, A Kobayashi, T Yamaki, H Kunii, K Nakazato, H Suzuki, T Ishida, Y Takeishi
    Open Heart, 2018-01-09;5(1):e000730.
    Species: Human
    Sample Types: Serum
  10. Elevated Serum Level of HMGB1 in Patients with the Antiphospholipid Syndrome
    Authors: V Manganelli, A Capozzi, S Truglia, C Alessandri, E Lococo, T Garofalo, C De Carolis, F Conti, G Valesini, M Sorice, A Longo, R Misasi
    J Immunol Res, 2017-12-19;2017(0):4570715.
    Species: Human
    Sample Types: Serum
  11. Orai and TRPC channel characterization in Fc?RI-mediated calcium signaling and mediator secretion in human mast cells
    Authors: HE Wajdner, J Farrington, C Barnard, PT Peachell, CG Schnackenb, JP Marino, X Xu, K Affleck, M Begg, EP Seward
    Physiol Rep, 2017-03-01;5(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Early B cell factor 1 regulates adipocyte morphology and lipolysis in white adipose tissue.
    Authors: Gao H, Mejhert N, Fretz J, Arner E, Lorente-Cebrian S, Ehrlund A, Dahlman-Wright K, Gong X, Stromblad S, Douagi I, Laurencikiene J, Dahlman I, Daub C, Ryden M, Horowitz M, Arner P
    Cell Metab, 2014-05-22;19(6):981-92.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Effect of renal impairment on cognitive function during a 3-year follow up in elderly patients with type 2 diabetes: Association with microinflammation.
    Authors: Kawamura T, Umemura T, Umegaki H, Imamine R, Kawano N, Tanaka C, Kawai M, Minatoguchi M, Kusama M, Kouchi Y, Watarai A, Kanai A, Nakashima E, Hotta N
    J Diabetes Investig, 2014-02-04;5(5):597-605.
    Species: Human
    Sample Types: Plasma
  14. Calcium sensing receptor activation elevates proinflammatory factor expression in human adipose cells and adipose tissue.
    Authors: Cifuentes M, Fuentes C, Tobar N, Acevedo I, Villalobos E, Hugo E, Ben-Jonathan N, Reyes M
    Mol Cell Endocrinol, 2012-03-16;361(1):24-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis.
    Authors: Narla A, Dutt S, McAuley JR
    Blood, 2011-04-28;118(8):2296-304.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Effect of intensive lifestyle changes on endothelial function and on inflammatory markers of atherosclerosis.
    Authors: Dod HS, Bhardwaj R, Sajja V, Weidner G, Hobbs GR, Konat GW, Manivannan S, Gharib W, Warden BE, Nanda NC, Beto RJ, Ornish D, Jain AC
    Am. J. Cardiol., 2010-02-01;105(3):362-7.
    Species: Human
    Sample Types: Serum
  17. Dexamethasone suppresses interleukin-22 associated with bacterial infection in vitro and in vivo.
    Authors: Ziesche E, Scheiermann P, Bachmann M, Sadik CD, Hofstetter C, Zwissler B, Pfeilschifter J, Muhl H
    Clin. Exp. Immunol., 2009-09-01;157(3):370-6.
    Species: Human
    Sample Types: Plasma
  18. Obesity in pregnancy stimulates macrophage accumulation and inflammation in the placenta.
    Authors: Challier JC, Basu S, Bintein T, Minium J, Hotmire K, Catalano PM, Hauguel-De Mouzon S
    Placenta, 2008-02-11;29(3):274-81.
    Species: Human
    Sample Types: Plasma
  19. Autocrine tumor necrosis factor alpha links endoplasmic reticulum stress to the membrane death receptor pathway through IRE1alpha-mediated NF-kappaB activation and down-regulation of TRAF2 expression.
    Authors: Hu P, Han Z, Couvillon AD, Kaufman RJ, Exton JH
    Mol. Cell. Biol., 2006-04-01;26(8):3071-84.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Effect of influenza vaccine on markers of inflammation and lipid profile.
    Authors: Tsai MY, Hanson NQ, Straka RJ, Hoke TR, Ordovas JM, Peacock JM, Arends VL, Arnett DK
    J. Lab. Clin. Med., 2005-06-01;145(6):323-7.
    Species: Human
    Sample Types: Plasma
  21. A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells.
    Authors: Nishishita T, Ouchi K, Zhang X, Inoue M, Inazawa T, Yoshiura K, Kuwabara K, Nakaoka T, Watanabe N, Igura K, Takahashi TA, Yamashita N
    Biochem. Biophys. Res. Commun., 2004-12-03;325(1):24-31.
    Species: Human
    Sample Types: Cell Lysates
  22. Possible contribution of adipocytokines on diabetic neuropathy.
    Authors: Matsuda M, Kawasaki F, Inoue H, Kanda Y, Yamada K, Harada Y, Saito M, Eto M, Matsuki M, Kaku K
    Diabetes Res. Clin. Pract., 2004-12-01;66(0):S121-3.
    Species: Human
    Sample Types: Plasma
  23. Impact of fibronectin fragments on the transendothelial migration of HIV-infected leukocytes and the development of subendothelial foci of infectious leukocytes.
    Authors: Birdsall HH, Porter WJ, Green DM, Rubio J, Trial J, Rossen RD
    J. Immunol., 2004-08-15;173(4):2746-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Plasma MMP-2 and MMP-9 and their inhibitors TIMP-1 and TIMP-2 during human orthotopic liver transplantation. The effect of aprotinin and the relation to ischemia/reperfusion injury.
    Authors: Kuyvenhoven JP, Molenaar IQ, Verspaget HW, Veldman MG, Palareti G, Legnani C, Moolenburgh SE, Terpstra OT, Lamers CB, van Hoek B, Porte RJ
    Thromb. Haemost., 2004-03-01;91(3):506-13.
    Species: Human
    Sample Types: Plasma
  25. Procalcitonin and proinflammatory cytokine clearance during continuous venovenous haemofiltration in septic patients.
    Authors: Dahaba AA, Elawady GA, Rehak PH, List WF
    Anaesth Intensive Care, 2002-06-01;30(3):269-74.
    Species: Human
    Sample Types: Plasma

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