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Human TIM-3 APC-conjugated Antibody

Catalog #: FAB2365A
15 Citations 1 Review Datasheet
Clone 344823 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB2365A
Detection of TIM‑3 in Human Blood Monocytes by Flow Cytometry.
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Citations (15)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TIM-3 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
Source
Monoclonal Rat IgG2A Clone # 344823
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIM‑3
Ser22-Arg200
Accession # Q8TDQ0.2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TIM‑3 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of TIM‑3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human TIM‑3 APC‑conjugated Monoclonal Antibody (Catalog # FAB2365A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human TIM-3 by Flow Cytometry View Larger

Detection of Human TIM-3 by Flow Cytometry Antigen Expression in AML and Normal Hematopoiesis. Antigen expression on primary AML samples at initial diagnosis and healthy donor-derived bone marrow cell populations (HD BM) as indicated in legend. a Representative primary AML sample and healthy donor-derived bone marrow sample. Histograms indicate fluorescence intensity. Numbers indicate MFI ratio. b Antigen expression in AML and normal hematopoiesis, shown as MFI ratio. Dots indicate measured samples. Violin plots illustrate distribution of antigen expression for each analyzed cell population. Black dotted line indicates MFI ratio of 1.5 as cutoff for positivity. c Summary of antigen expression levels (median MFI ratio) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29946192), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TIM-3

TIM-3 (T cell Immunoglobulin and Mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 6). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (7). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 6, 8), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (9).

References
  1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
  4. Monney, L. et al. (2002) Nature 415:536.
  5. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  6. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  7. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  8. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
  9. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
Long Name
T Cell Immunoglobulin Mucin-3
Entrez Gene IDs
84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)
Alternate Names
CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3

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Citations for Human TIM-3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Influence of Galectin-9 Treatment on the Phenotype and Function of NK-92MI Cells in the Presence of Different Serum Supplements
    Authors: Matyas Meggyes, David U Nagy, Timea Balassa, Krisztina Godony, Agnes Peterfalvi, Laszlo Szereday et al.
    Biomolecules
  2. Efficient lentiviral transduction method to gene modify cord blood CD8+ T cells for cancer therapy applications
    Authors: Vania Lo Presti, Annelisa M. Cornel, Maud Plantinga, Ester Dünnebach, Jurgen Kuball, Jaap Jan Boelens et al.
    Molecular Therapy - Methods & Clinical Development
  3. Nivolumab plus ipilimumab in advanced salivary gland cancer: a phase 2 trial
    Authors: Vos, JL;Burman, B;Jain, S;Fitzgerald, CWR;Sherman, EJ;Dunn, LA;Fetten, JV;Michel, LS;Kriplani, A;Ng, KK;Eng, J;Tchekmedyian, V;Haque, S;Katabi, N;Kuo, F;Han, CY;Nadeem, Z;Yang, W;Makarov, V;Srivastava, RM;Ostrovnaya, I;Prasad, M;Zuur, CL;Riaz, N;Pfister, DG;Klebanoff, CA;Chan, TA;Ho, AL;Morris, LGT;
    Nature medicine
    Species: Human
    Sample Types: Whole Cell
    Applications: Flow Cytometry
  4. Phenotype and Reactivity of Lymphocytes Expanded from Benign Prostate Hyperplasic Tissues and Prostate Cancer
    Authors: Ahmed, R;Lozano, LE;Anastasio, A;Lofek, S;Mastelic-Gavillet, B;Navarro Rodrigo, B;Nguyen, S;Dartiguenave, F;Rodrigues-Dias, SC;Cesson, V;Valério, M;Roth, B;Kandalaft, LE;Redchenko, I;Hill, AVS;Harari, A;Romero, P;Derré, L;Viganó, S;
    Cancers
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. CD226 opposes TIGIT to disrupt Tregs in melanoma
    Authors: J Fourcade, Z Sun, JM Chauvin, M Ka, D Davar, O Pagliano, H Wang, S Saada, C Menna, R Amin, C Sander, JM Kirkwood, AJ Korman, HM Zarour
    JCI Insight, 2018-07-26;3(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Antitumor Activity Associated with Prolonged Persistence of Adoptively Transferred NY-ESO-1c259T cells in Synovial Sarcoma
    Authors: SP D'Angelo, L Melchiori, MS Merchant, DB Bernstein, J Glod, RN Kaplan, SA Grupp, WD Tap, K Chagin, GK Binder, S Basu, DE Lowther, R Wang, N Bath, A Tipping, G Betts, I Ramachandr, JM Navenot, H Zhang, DK Wells, E Van Winkle, G Kari, T Trivedi, T Holdich, LN Pandite, R Amado, CL Mackall
    Cancer Discov, 2018-06-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. T-cell invigoration to tumour burden ratio associated with anti-PD-1 response
    Authors: AC Huang, MA Postow, RJ Orlowski, R Mick, B Bengsch, S Manne, W Xu, S Harmon, JR Giles, B Wenz, M Adamow, D Kuk, KS Panageas, C Carrera, P Wong, F Quagliarel, B Wubbenhors, K D'Andrea, KE Pauken, RS Herati, RP Staupe, JM Schenkel, S McGettigan, S Kothari, SM George, RH Vonderheid, RK Amaravadi, GC Karakousis, LM Schuchter, X Xu, KL Nathanson, JD Wolchok, TC Gangadhar, EJ Wherry
    Nature, 2017-04-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Circulating T lymphocyte subsets, cytokines, and immune checkpoint inhibitors in patients with bipolar II or major depression: a preliminary study
    Authors: W Wu, YL Zheng, LP Tian, JB Lai, CC Hu, P Zhang, JK Chen, JB Hu, ML Huang, N Wei, WJ Xu, WH Zhou, SJ Lu, J Lu, HL Qi, DD Wang, XY Zhou, JF Duan, Y Xu, SH Hu
    Sci Rep, 2017-01-11;7(0):40530.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. TIM-3/Gal-9 interaction induces IFNgamma-dependent IDO1 expression in acute myeloid leukemia blast cells.
    Authors: Folgiero V, Cifaldi L, Li Pira G, Goffredo B, Vinti L, Locatelli F
    J Hematol Oncol, 2015-04-16;8(0):36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Inhibitory phenotype of HBV-specific CD4+ T-cells is characterized by high PD-1 expression but absent coregulation of multiple inhibitory molecules.
    Authors: Raziorrouh B, Heeg M, Kurktschiev P, Schraut W, Zachoval R, Wendtner C, Wachtler M, Spannagl M, Denk G, Ulsenheimer A, Bengsch B, Pircher H, Diepolder H, Gruner N, Jung M
    PLoS ONE, 2014-08-21;9(8):e105703.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy.
    Authors: Meggyes, Matyas, Miko, Eva, Polgar, Beata, Bogar, Barbara, Farkas, Balint, Illes, Zsolt, Szereday, Laszlo
    PLoS ONE, 2014-03-20;9(3):e92371.
    Species: Human
    Sample Types:
  12. Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection.
    Authors: Wang, Jia M, Shi, Lei, Ma, Cheng J, Ji, Xiao J, Ying, Ruo S, Wu, Xiao Y, Wang, Ke S, Li, Guangyu, Moorman, Jonathan, Yao, Zhi Q
    J Virol, 2013-02-06;87(8):4372-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Blockade of Tim-3 signaling restores the virus-specific CD8+ T-cell response in patients with chronic hepatitis B.
    Authors: Wu W, Shi Y, Li S, Zhang Y, Liu Y, Wu Y, Chen Z
    Eur. J. Immunol., 2012-05-01;42(5):1180-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Tim-3 negatively regulates IL-12 expression by monocytes in HCV infection.
    Authors: Zhang Y, Ma CJ, Wang JM, Ji XJ, Wu XY, Jia ZS, Moorman JP, Yao ZQ
    PLoS ONE, 2011-05-26;6(5):e19664.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. PEGylated IL-10 (Pegilodecakin) Induces Systemic Immune Activation, CD8+ T Cell Invigoration and Polyclonal T Cell Expansion in Cancer Patients
    Authors: Aung Naing, Jeffrey R. Infante, Kyriakos P. Papadopoulos, Ivan H. Chan, Cong Shen, Navneet P. Ratti et al.
    Cancer Cell

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Human TIM-3 APC-conjugated Antibody
By Vikram Mavinkurve on 05/10/2018
Application: Flow Sample Tested: B cell enriched peripheral blood mononuclear cells (PBMC) Species: Human
Flow Cytometry Human TIM-3 APC-conjugated Antibody FAB2365A