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Human TIM-3 Antibody

Catalog #: MAB2365
15 Citations Datasheet
Clone 344823 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB2365
MAB2365-SP
Detection of TIM‑3 in Human Monocytes by Flow Cytometry.
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Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TIM-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TIM‑3 in direct ELISAs. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
Source
Monoclonal Rat IgG2A Clone # 344823
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIM‑3
Ser22-Arg200
Accession # Q8TDQ0.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-reported
Amir, el-A.D. et al. (2013) Nature Biotechnol. 31: 545. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TIM‑3 antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of TIM‑3 in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human TIM-3 Monoclonal Antibody (Catalog # MAB2365, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-3

TIM-3 (T cell Immunoglobulin and Mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 6). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (7). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 6, 8), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (9).

References
  1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
  4. Monney, L. et al. (2002) Nature 415:536.
  5. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
  6. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
  7. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  8. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
  9. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
Long Name
T Cell Immunoglobulin Mucin-3
Entrez Gene IDs
84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)
Alternate Names
CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3

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Citations for Human TIM-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. T cell-mediated elimination of cancer cells by blocking CEACAM6–CEACAM1 interaction
    Authors: Jessica Pinkert, Hans-Henning Boehm, Mark Trautwein, Wolf-Dietrich Doecke, Florian Wessel, Yingzi Ge et al.
    OncoImmunology
  2. Using the circulating proteome to assess type I interferon activity in systemic lupus erythematosus
    Authors: MA Smith, CC Chiang, K Zerrouki, S Rahman, WI White, K Streicher, WA Rees, A Schiffenba, LG Rider, FW Miller, Z Manna, S Hasni, MJ Kaplan, R Siegel, D Sinibaldi, MA Sanjuan, KA Casey
    Sci Rep, 2020-03-10;10(1):4462.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Reduced CD160 expression contributes to impaired NK cell function and poor clinical outcomes in patients with HCC
    Authors: H Sun, J Xu, Q Huang, M Huang, K Li, K Qu, H Wen, R Lin, M Zheng, H Wei, W Xiao, R Sun, Z Tian, C Sun
    Cancer Res., 2018-09-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Inhibition of AKT signaling uncouples T cell differentiation from expansion for receptor-engineered adoptive immunotherapy
    Authors: CA Klebanoff, JG Crompton, AJ Leonardi, TN Yamamoto, SS Chandran, RL Eil, M Sukumar, SK Vodnala, J Hu, Y Ji, D Clever, MA Black, D Gurusamy, MJ Kruhlak, P Jin, DF Stroncek, L Gattinoni, SA Feldman, NP Restifo
    JCI Insight, 2017-12-07;2(23):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells
    Authors: C Stecher, C Battin, J Leitner, M Zettl, K Grabmeier-, C Höller, GJ Zlabinger, P Steinberge
    Front Immunol, 2017-05-22;8(0):572.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9
    Authors: JA Schwartz, KL Clayton, S Mujib, H Zhang, AK Rahman, J Liu, FY Yue, E Benko, C Kovacs, MA Ostrowski
    J. Immunol, 2017-03-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. PD-1/PD-L1 blockade enhances T cell activity and antitumor efficacy of imatinib in gastrointestinal stromal tumors
    Clin Cancer Res, 2016-07-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition
    J Clin Invest, 2016-07-25;126(8):3130-44.
    Species: Human
    Sample Types: Whole Cells
  9. The neonatal Fc receptor (FcRn) binds independently to both sites of the IgG homodimer with identical affinity
    Authors: Yasmina Noubia Abdiche, Yik Andy Yeung, Javier Chaparro-Riggers, Ishita Barman, Pavel Strop, Sherman Michael Chin et al.
    mAbs
  10. Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression.
    Authors: Clayton K, Douglas-Vail M, Nur-ur Rahman A, Medcalf K, Xie I, Chew G, Tandon R, Lanteri M, Norris P, Deeks S, Ndhlovu L, Ostrowski M
    J Virol, 2015-01-21;89(7):3723-36.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. A Novel Soluble Form of Tim-3 Associated with Severe Graft-versus-Host Disease
    Authors: John A. Hansen, Samir M. Hanash, Laura Tabellini, Chris Baik, Richard L. Lawler, Bryan M. Grogan et al.
    Biology of Blood and Marrow Transplantation
  12. Galectin-9 functionally impairs natural killer cells in humans and mice.
    Authors: Golden-Mason L, McMahan R, Strong M, Reisdorph R, Mahaffey S, Palmer B, Cheng L, Kulesza C, Hirashima M, Niki T, Rosen H
    J Virol, 2013-02-13;87(9):4835-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Molecular profiling of cytomegalovirus-induced human CD8+ T cell differentiation.
    Authors: Hertoghs KM, Moerland PD, van Stijn A, Remmerswaal EB, Yong SL, van de Berg PJ, van Ham SM, Baas F, ten Berge IJ, van Lier RA
    J. Clin. Invest., 2010-11-01;120(11):4077-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. HCV-specific T cells in HCV/HIV co-infection show elevated frequencies of dual Tim-3/PD-1 expression that correlate with liver disease progression.
    Authors: Vali B, Jones RB, Sakhdari A
    Eur. J. Immunol., 2010-09-01;40(9):2493-505.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Structural features of galectin-9 and galectin-1 that determine distinct T cell death pathways.
    Authors: Bi S, Earl LA, Jacobs L, Baum LG
    J. Biol. Chem., 2008-02-07;283(18):12248-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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