Human TIM-3 Alexa Fluor® 700-conjugated Antibody
Human TIM-3 Alexa Fluor® 700-conjugated Antibody Summary
Ser22-Arg200
Accession # Q8TDQ0.2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TIM‑3 in Human PBMCs by Flow Cytometry. Human peripheral blood mono-nuclear cells (PBMCs) were stained with Rat Anti-Human TIM-3 Alexa Fluor® 700-conjugated Mono-clonal Antibody (Catalog # FAB2365N) and Mouse Anti-Human CD14 PE-conjugated Mono-clonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.
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Detection of Human TIM-3 by Flow Cytometry Upregulation of immune checkpoint ligands on T cells after dendritic cell stimulation. T cells of 7–14 healthy donor were cocultured with autologous TLR-3-DCs pulsed with CMV, EBV, influenza, tetanus (CEFT) peptide pool or with CEFT peptide pool alone. Expression of various inhibitory checkpoint molecules was analyzed by flow cytometry. The percentage of positive cells is presented as box-and-whisker plots for CD4+(A) and for CD8+(B) T cells. *p < 0.05; **p < 0.01. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fimmu.2018.00385/full), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TIM-3
TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). There are three TIM genes in human and eight in mouse. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. It also occurs on hippocampal neurons (7-10). The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 11). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (8, 12). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 10, 11, 13), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (14). Soluble mouse TIM-3 constructs which lack the cytoplasmic domain have been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (5, 15).
- Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
- Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
- Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
- Monney, L. et al. (2002) Nature 415:536.
- Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
- Khademi, M. et al. (2004) J. Immunol. 172:7169.
- Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
- van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
- Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
- Oikawa, T. et al. (2006) J. Immunol. 177:4281.
- Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
- Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
- Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
- Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
- Geng, H. et al. (2006) J. Immunol. 176:1411.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citations for Human TIM-3 Alexa Fluor® 700-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Human effector CD8+ T cells with an activated and exhausted-like phenotype control tumour growth in vivo in a humanized tumour model
Authors: Mietz, J;Kaulfuss, M;Egli, L;Opitz, L;Münz, C;Chijioke, O;
EBioMedicine
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Primary T Cells from Cutaneous T-cell Lymphoma Skin Explants Display an Exhausted Immune Checkpoint Profile
Authors: Christiane Querfeld, Samantha Leung, Patricia L. Myskowski, Shane A. Curran, Debra A. Goldman, Glenn Heller et al.
Cancer Immunology Research
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Effect of IL-7 Therapy on Phospho-Ribosomal Protein S6 and TRAF1 Expression in HIV-Specific CD8 T Cells in Patients Receiving Antiretroviral Therapy
Authors: C Wang, MI Edilova, LE Wagar, S Mujib, M Singer, NF Bernard, T Croughs, MM Lederman, I Sereti, MA Fischl, E Kremmer, M Ostrowski, JP Routy, TH Watts
J. Immunol., 2017-12-08;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Increased frequency of Tim-3 expressing T cells is associated with symptomatic West Nile virus infection.
Authors: Lanteri M, Diamond M, Law J, Chew G, Wu S, Inglis H, Wong D, Busch M, Norris P, Ndhlovu L
PLoS ONE, 2014-03-18;9(3):e92134.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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