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Human Tie-2 APC-conjugated Antibody

Catalog #: FAB3131A
12 Citations Datasheet
Catalog # Availability Size / Price Qty
FAB3131A
Detection of Tie‑2 in HUVEC Human Cells by Flow Cytometry.
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Product Details
Citations (12)
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Human Tie-2 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Tie-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Tie‑1, recombinant mouse Tie-2, or recombinant zebrafish Tie-2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 83715
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tie‑2
Ala23-Lys745
Accession # AAA61139
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Tie-2 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of Tie‑2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Tie-2 APC-conjugated Monoclonal Antibody (Catalog # FAB3131A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

References
  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Takakura, N. et al. (1998) Immunity 9:677.
  3. Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Entrez Gene IDs
7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)
Alternate Names
angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; Tie2; Tie-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1

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Citations for Human Tie-2 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Monocyte Subpopulations from Pre-Eclamptic Patients Are Abnormally Skewed and Exhibit Exaggerated Responses to Toll-Like Receptor Ligands
    Authors: Ebtisam Al-ofi, Seth B. Coffelt, Dilly O. Anumba
    PLoS ONE
  2. Successful fishing for nucleus pulposus progenitor cells of the intervertebral disc across species
    Authors: Daisuke Sakai, Jordy Schol, Frances C. Bach, Adel Tekari, Nobuho Sagawa, Yoshihiko Nakamura et al.
    JOR SPINE
  3. Distinct RNA transcriptome patterns are potentially associated with angiogenesis in Tie2-expressing monocytes
    Authors: Xinjing Wang, Zhiyuan Dai, Xiaoli Wu, Kai Wang, Xipeng Wang
    Gene
  4. Assessment of Tie2-Rejuvenated Nucleus Pulposus Cell Transplants from Young and Old Patient Sources Demonstrates That Age Still Matters
    Authors: Otani, Y;Schol, J;Sakai, D;Nakamura, Y;Sako, K;Warita, T;Tamagawa, S;Ambrosio, L;Munesada, D;Ogasawara, S;Matsushita, E;Kawachi, A;Naiki, M;Sato, M;Watanabe, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. SOD2 orchestrates redox homeostasis in intervertebral discs: A novel insight into oxidative stress-mediated degeneration and therapeutic potential
    Authors: Tamagawa, S;Sakai, D;Nojiri, H;Nakamura, Y;Warita, T;Matsushita, E;Schol, J;Soma, H;Ogasawara, S;Munesada, D;Koike, M;Shimizu, T;Sato, M;Ishijima, M;Watanabe, M;
    Redox biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. TNF-&alpha induces endothelial-mesenchymal transition promoting stromal development of pancreatic adenocarcinoma
    Authors: M Adjuto-Sac, P Soubeyran, J Garcia, S Audebert, L Camoin, M Rubis, J Roques, B Binétruy, JL Iovanna, R Tournaire
    Cell Death & Disease, 2021-06-25;12(7):649.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Tie2 Expressing Monocytes in the Spleen of Patients with Primary Myelofibrosis
    Authors: Rita Campanelli
    PLoS ONE, 2016-06-09;11(6):e0156990.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib.
    Authors: Fiorio Pla, Alessand, Brossa, Alessia, Bernardini, Michela, Genova, Tullio, Grolez, Guillaum, Villers, Arnaud, Leroy, Xavier, Prevarskaya, Natalia, Gkika, Dimitra, Bussolati, Benedett
    BMC Cancer, 2014-12-12;14(0):939.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. TGFbeta inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy.
    Authors: Wang C, Tang X, Sun X, Miao Z, Lv Y, Yang Y, Zhang H, Zhang P, Liu Y, DU L, Gao Y, Yin M, Ding M, Deng H
    Cell Res., 2011-08-23;22(1):194-207.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
    Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
    J. Thromb. Haemost., 2011-05-01;9(5):1056-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Decrease of Pro-Angiogenic Monocytes Predicts Clinical Response to Anti-Angiogenic Treatment in Patients with Metastatic Renal Cell Carcinoma
    Authors: Stephane Oudard, Nadine Benhamouda, Bernard Escudier, Patrice Ravel, Thi Tran, Emeline Levionnois et al.
    Cells
  12. Tie2-Expressing Monocytes Are Associated with Identification and Prognoses of Hepatitis B Virus Related Hepatocellular Carcinoma after Resection
    Authors: Yi-Feng He, Chao-Qun Wang, Yao Yu, Jing Qian, Kang Song, Qi-Man Sun et al.
    PLOS ONE

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