Human Tie-2 Antibody Summary
Ala23-Lys745
Accession # AAA61139
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tie-2
Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.
- Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
- Takakura, N. et al. (1998) Immunity 9:677.
- Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
Product Datasheets
Citations for Human Tie-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Acidic pH reduces VEGF-mediated endothelial cell responses by downregulation of VEGFR-2; relevance for anti-angiogenic therapies
Authors: Seraina Faes, Emilie Uldry, Anne Planche, Tania Santoro, Catherine Pythoud, Nicolas Demartines et al.
Oncotarget
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Down-regulation of vascular endothelial growth factor receptor 2 is a major molecular determinant of proteasome inhibitor-mediated antiangiogenic action in endothelial cells.
Authors: Meissner M, Reichenbach G, Stein M, Hrgovic I, Kaufmann R, Gille J
Cancer Res., 2009-02-17;69(5):1976-84.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Monoclonal antibody classification based on epitope-binding using differential antigen disruption.
Authors: Shi E, Fury W, Li W, Mikulka W, Aldrich T, Rafique A, Chen G, Hoffenberg S, Daly TJ, Radziejewski C
J. Immunol. Methods, 2006-06-27;314(1):9-20.
Species: Human
Sample Types: Recombinant Protein
Applications: Biacore Binding Assay, Epitope Mapping -
Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V
Blood, 2003-03-20;102(2):638-45.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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