Human Th1/Th2 Luminex® Performance Assay 13-plex Fixed Panel

Catalog #: LKTM008B
1 Citation Datasheet

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LKTM008B

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Human Th1/Th2 Cytokine Panel Standard Curves

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Summary Product Features Precision & Accuracy Data Examples Product Datasheets Background

Human Th1/Th2 Luminex® Performance Assay 13-plex Fixed Panel Summary

Assay Type	bead-based multiplex assay for the Luminex^® platform
Format	1 x 96-well microplate and antibody-coated beads
Analytes Detected	Please see analyte list in assay customization tool below.
Performance Validation	Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Ordering Tool. We also offer Custom Luminex Services

Product Features

Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.

Simultaneously profile up to 50 analytes of your choice in one sample
Choose from a wide menu of analytes, including many unique analytes
Requires a small sample volume (<50 µL)
Run your assay in just 3-4 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Please allow additional time for international orders.

Kit Components

Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Standard/Sample Diluent
Wash Buffer
Streptavidin-PE
Foil Plate Sealers (4)
Mixing Bottle
Certificate of Analysis**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Precision

	n=40 (20x2 levels)	n=30
Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 30 different serum assays.
Analyte	Intra-Assay (%CV)	Inter-Assay (%CV)
GM-CSF	3.4	11.2
IFN-gamma	2.3	10.9
IL-1 beta	2.8	10.1
IL-2	3.9	9.6
IL-4	2.4	8.4
IL-5	1.8	8.3
IL-6	2.2	8.8
IL-9	6.2	10.3
IL-10	3.6	11.2
IL-12 p70	2.6	9.8
IL-13	2.5	8.3
TNF-alpha	3.0	10.3
TNF-beta	2.2	8.0

Accuracy

	Linearity		Spiked Recovery
Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
Analyte	N	Average %	N	Average %
GM-CSF	4	101	4	98
IFN-gamma	4	127	4	73
IL-1 beta	4	106	4	94
IL-2	4	115	4	88
IL-4	4	141	4	58
IL-5	4	113	4	88
IL-6	4	126	4	78
IL-9	4	111	4	96
IL-10	4	117	4	90
IL-12 p70	4	111	4	87
IL-13	4	118	4	89
TNF-alpha	4	134	4	71
TNF-beta	4	131	4	65

Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

Scientific Data

N/A

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Human Th1/Th2 Cytokine Panel Standard Curves

Background: Th1/Th2 Assay Kits

T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After activation, T helper cells differentiate into T-helper (Th) 1 or Th2 effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.
Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized—some cytokines are more effective than others in the process of eliminating certain invaders

Alternate Names

Th1/Th2 Assay Kits

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Product Specific Notices

Luminex® is a registered trademark of Luminex Corporation.

Citation for Human Th1/Th2 Luminex® Performance Assay 13-plex Fixed Panel

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

IL-12 reprograms CAR-expressing natural killer T cells to long-lived Th1-polarized cells with potent antitumor activity
Authors: Landoni, E;Woodcock, MG;Barragan, G;Casirati, G;Cinella, V;Stucchi, S;Flick, LM;Withers, TA;Hudson, H;Casorati, G;Dellabona, P;Genovese, P;Savoldo, B;Metelitsa, LS;Dotti, G;
Nature communications
Species: Mouse
Sample Types: Serum, Plasma