Human TGF-beta RIII Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB2421U Datasheet

Catalog #	Availability	Size / Price	Qty
FAB2421U-100UG

All TGF-beta RIII Antibodies

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Human TGF-beta RIII Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TGF-beta RIII in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) TGF-beta RI, RII, RIIb, recombinant mouse (rm) TGF-beta RIII, rhTGF-beta 1, beta 1.2, beta 2, beta 3, rhLatent TGF

Source

Monoclonal Mouse IgG1 Clone # 31308

Purification

Protein A or G purified

Immunogen

Murine myeloma cell line, NS0-derived recombinant human TGF-Î² RIII
Gly21-Asp781
Accession # AAA67061

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TGF-beta RIII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta. The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.

Long Name

Transforming Growth Factor beta Receptor III

Entrez Gene IDs

7049 (Human); 21814 (Mouse); 29610 (Rat)

Alternate Names

betaglycan proteoglycan; Betaglycan; BGCAN; TBRIII; TGF-beta receptor type 3; TGF-beta receptor type III; TGF-beta RIII; TGFbetaRIII; TGFBR3; TGF-bRIII; TGFR-3; Transforming growth factor beta receptor III; transforming growth factor beta receptor type 3; transforming growth factor, beta receptor III (betaglycan, 300kDa); transforming growth factor, beta receptor III