Human TGF-beta RII Isoform 2 Antibody

Catalog #: AF1003 Datasheet

Catalog #	Availability	Size / Price	Qty
AF1003
AF1003-SP

All TGF-beta RII Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TGF-beta RII Isoform 2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TGF‑ beta RII Isoform 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) TGF-beta RII, recombinant mouse (rm) TGF-beta RII, rmTGF-beta RI, and rhTGF-beta RIII is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta RII Isoform 2
Thr23-Asp184
Accession # NP_001020018

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TGF‑ beta RII Isoform 2 Fc Chimera (Catalog # 1003-RT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa) and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3, and appears to participate in both TGF-beta dependent and independent cellular signaling. The Type II receptor, by contrast, is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity, and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that requires the presence of the Type II receptor to bind TGF-beta. Evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors. TGF-beta receptor II isoform 2 (also TGF-beta RII isoform A) is an alternatively spliced variant of the standard Type II TGF-beta receptor (or TGF-beta RII isoform 1) that possesses a 27 amino acid substitution for Val10 near the N-terminus of the mature protein. Both TGF-beta RII and TGF-beta RII isoform 2 bind TGF-beta 1 and TGF-beta 3 with high affinity. However, only TGF-beta RII isoform 2 also binds TGF-beta 2 with high affinity in the absence of TGF-beta RIII. While TGF-beta RII is widely expressed on cells, TGF-beta RII isoform 2 shows distinct expression pattern mainly associated with bone-related cells, such as osteoblasts and mesenchymal precursor cells. TGF-beta RII isoform 2 may play an important role in TGF-beta 2 binding and signaling in cells lacking TGF-beta RIII. Mouse also expresses a TGF-beta RII isoform 2, and over aa 23-184 of this isoform, human and mouse share 80% aa sequence identity.

Long Name

Transforming Growth Factor beta Receptor II

Entrez Gene IDs

7048 (Human); 21813 (Mouse)

Alternate Names

AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II