Human TGF-beta RII Fluorescein-conjugated Antibody

Catalog #: FAB2411F 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
FAB2411F-100
FAB2411F-025

Detection of TGF‑ beta RII in Human Blood Lymphocytes by Flow Cytometry.

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All TGF-beta RII Antibodies

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Human TGF-beta RII Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) TGF‑ beta RII and less than 1% cross-reactivity with recombinant human TGF-beta RIII and rmTGF-beta RI is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta RII
Ile24-Asp159
Accession # P37173.2

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TGF‑ beta RII in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Goat Anti-Human TGF-beta RII Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2411F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta RII

TGF-beta RII is a membrane bound serine/threonine kinase. Upon ligand binding, TGF-beta RII interacts with TGF-beta RI to form the heteromeric signaling complex that transduces TGF-beta signals. A splice variant of the type II receptor, TGF-beta RIIb, containing a 25 amino acid residue insertion near the N-terminus of the mature protein has also been described.

Long Name

Transforming Growth Factor beta Receptor II

Entrez Gene IDs

7048 (Human); 21813 (Mouse)

Alternate Names

AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II