Human STING/TMEM173 Antibody

Detection of Human STING/TMEM173 by Western Blot. Western blot shows lysates of human peripheral blood lymphocytes (PBL). PVDF Membrane was probed with 1 µg/mL of Human/Mouse STING/TMEM173 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6516) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for STING/TMEM173 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human STING/TMEM173 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STING/TMEM173 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for STING/TMEM173 at approximately 41 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. Sheep Anti-Human STING/TMEM173 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6516) was used at 10 µg/mL followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse Human STING/TMEM173 Antibody by Immunohistochemistry STING-mediated effects on vascular remodeling are due to NETs. i, j Confocal images of CD31-positive microvessels (i) and quantification of microvascular density (j) in the peri-infarct cortex at 14 days in mice treated with control IgG or IFNAR-neutralizing antibody, and STING shRNA or control adenovirus (n = 6), unpaired two-tailed Student’s t-test was applied with *P < 0.0001 (Vehicle vs. IFNAR), *P = 0.0007 (Ad-con vs. Ad-sh-STING). Bar = 40 μm. k, l In-vivo multiphoton microscopic images of perfused cortical capillaries with intravenously injected FITC-dextran (k) and quantification of perfused capillary length (l) at 14 days after stroke (n = 6), unpaired two-tailed Student’s t-test was applied with *P = 0.0006 (Vehicle vs. IFNAR), *P = 0.0080 (Ad-con vs. Ad-sh-STING). Bar = 100 μm. m, n In-vivo multiphoton microscopic images (m) of intravenously injected FITC-dextran leakage in cortical vessels at 14 days and quantification of the permeability (P) product of FITC-dextran (n) for each group (n = 6), unpaired two-tailed Student’s t-test was applied with *P = 0.0028 (Vehicle vs. IFNAR), *P = 0.0053 (Ad-con vs. Ad-sh-STING). Bar = 100 μm. Data are presented as mean ± SD. Source data underlying graph a–h, j, l, and n are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Human STING/TMEM173 Antibody by Western Blot STING-mediated effects on vascular remodeling are due to NETs. f Immunoblot analysis of STING, pTBK1, and pIRF3 in the ischemic cortex at day 3. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Human STING/TMEM173 Antibody by Western Blot STING-mediated effects on vascular remodeling are due to NETs. b Immunoblot analysis of STING, phosphorylated TBK1 (pTBK1), and pIRF3 in the cortex of mice without stroke or at day 3 after stroke. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Human STING/TMEM173 Antibody by Western Blot STING-mediated effects on vascular remodeling are due to NETs. d Immunoblot analysis of STING, pTBK1, and pIRF3 in the ischemic cortex at day 3. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Human STING/TMEM173 Antibody by Western Blot STING-mediated effects on vascular remodeling are due to NETs.h Immunoblot analysis of STING, pTBK1, and pIRF3 in isolated neutrophils for each group (n = 5). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Human STING/TMEM173 Antibody by Immunohistochemistry STING-mediated effects on vascular remodeling are due to NETs. k, l In-vivo multiphoton microscopic images of perfused cortical capillaries with intravenously injected FITC-dextran (k) and quantification of perfused capillary length (l) at 14 days after stroke (n = 6), unpaired two-tailed Student’s t-test was applied with *P = 0.0006 (Vehicle vs. IFNAR), *P = 0.0080 (Ad-con vs. Ad-sh-STING). Bar = 100 μm. m, n In-vivo multiphoton microscopic images (m) of intravenously injected FITC-dextran leakage in cortical vessels at 14 days and quantification of the permeability (P) product of FITC-dextran (n) for each group (n = 6), unpaired two-tailed Student’s t-test was applied with *P = 0.0028 (Vehicle vs. IFNAR), *P = 0.0053 (Ad-con vs. Ad-sh-STING). Bar = 100 μm. Data are presented as mean ± SD. Source data underlying graphs are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Human STING/TMEM173 Antibody by Immunohistochemistry STING-mediated effects on vascular remodeling are due to NETs. m, n In-vivo multiphoton microscopic images (m) of intravenously injected FITC-dextran leakage in cortical vessels at 14 days and quantification of the permeability (P) product of FITC-dextran (n) for each group (n = 6), unpaired two-tailed Student’s t-test was applied with *P = 0.0028 (Vehicle vs. IFNAR), *P = 0.0053 (Ad-con vs. Ad-sh-STING). Bar = 100 μm. Data are presented as mean ± SD. Source data underlying graphs are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32427863), licensed under a CC-BY license. Not internally tested by R&D Systems.