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Human Smoothened Antibody

Catalog #: MAB10276 Datasheet
Catalog # Availability Size / Price Qty
MAB10276-100
MAB10276-SP
Smoothened in Human Liver Cancer Tissue.
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Human Smoothened Antibody Summary

Species Reactivity
Human
Specificity
Detects human Smoothened in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 1015418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human Smoothened
Met1-Tyr233
Accession # Q99835
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Smoothened antibody in Human Liver Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Smoothened in Human Liver Cancer Tissue. Smoothened was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human Smoothened Monoclonal Antibody (Catalog # MAB10276) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Smoothened

Smoothened homolog (SMO) is a G protein-coupled receptor that is a component of the hedgehog signaling pathway. SMO can function as an oncogene. Activating SMO mutations can lead to unregulated activation of the hedgehog pathway and serve as driving mutations for cancers such as medulloblastoma, basal-cell carcinoma, pancreatic cancer, and prostate cancer. As such, SMO is an attractive cancer drug target, along with the many hedgehog pathway agonists and antagonists that are known to directly target SMO.

Entrez Gene IDs
6608 (Human); 319757 (Mouse); 25273 (Rat)
Alternate Names
bnb; CRJS; FZD11; Gx; Protein Gx; SMO; SMOH; SMOHseven transmembrane helix receptor; smoothened (Drosophila) homolog; smoothened homolog (Drosophila); smoothened homolog; Smoothened

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