Human SHANK2 Alexa Fluor® 350-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: SHANK2

SHANK2 (SH3 and multiple ANKyrin repeat domain protein 2; also CortBP1 and ProSAP1) is a 180-200 kDa member of the Shank family of proteins.  It is expressed in neurons and various epithelial cells, and serves as a multidomain scaffold for cell membrane proteins. For example, in the post-synaptic density of neurons, it provides support for the interaction between glutamate receptors and the actin cytoskeleton. In epithelia, SHANK2 regulates the trafficking and activity of Na/H Exchanger 3. Human SHANK2 is 1470 amino acids (aa) in length. It contains an N-terminal SH3 domain (aa 147-206), a PDZ domain (aa 247-341), a Pro-rich region (aa 509-535), a potential O-linked glycosylation site (aa 948-954) and a C-terminal SAM domain (aa 1407-1470). There are seven potential Ser/Thr phosphorylation sites. Multiple splice forms exist. A 220-240 kDa ankyrin repeat-containing isoform (SHANK2-E) possesses a 391 aa substitution for aa 1-12, and a 165 kDa isoform (potentially known as CortBP1) shows a 28 aa substitution for aa 1-238 coupled with a deletion of aa 383-390. Another potential isoform shows the previously noted 28 aa substitution for aa 1-238 coupled to a 19 aa substitution for aa 472-1470. Over aa 849-1029, human SHANK2 shares 80% aa sequence identity with mouse SHANK2.

Product Datasheets

Related Research Areas

• Adaptor Proteins
• Neuronal Markers
• Synaptic Proteins