Human Serpin E1/PAI-1 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, CTAD Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (ng/mL) | 2.6 | 6.5 | 12.6 | 2.3 | 6.4 | 13.1 |
Standard Deviation | 0.12 | 0.52 | 0.98 | 0.2 | 0.4 | 0.8 |
CV% | 4.4 | 8 | 7.8 | 9.5 | 6.4 | 6.1 |
Recovery
The recovery of Serpin E1 spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 106 | 89-113 |
Citrate Plasma (n=4) | 99 | 88-112 |
CTAD Plasma (n=4) | 100 | 83-112 |
EDTA Plasma (n=4) | 100 | 85-115 |
Heparin Plasma (n=4) | 98 | 85-113 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Serpin E1/PAI-1
The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Serpin E1/PAI-1 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Association of circulating biomarkers with illness severity measures differentiates myalgic encephalomyelitis/chronic fatigue syndrome and post-COVID-19 condition: a prospective pilot cohort study
Authors: Domingo, JC;Battistini, F;Cordobilla, B;Zaragozá, MC;Sanmartin-Sentañes, R;Alegre-Martin, J;Cambras, T;Castro-Marrero, J;
Journal of translational medicine
Species: Human
Sample Types: Plasma
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SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection
Authors: Richards, A;Khalil, A;Friesen, M;Whitfield, TW;Lungjangwa, T;Gehrke, L;Mooney, D;Jaenisch, R;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Cell Culture Supernates
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The Role of Plasminogen Activator Inhibitor 1 in Predicting Sepsis-Associated Liver Dysfunction: An Observational Study
Authors: E Wo?nica-Ni, P Le?nik, J Janc, M Zalewska, L ?ysenko
International Journal of Environmental Research and Public Health, 2023-03-09;20(6):.
Species: Human
Sample Types: Plasma
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Coordinated reprogramming of renal cancer transcriptome, metabolome and secretome associates with immune tumor infiltration
Authors: P Poplawski, S Alseekh, U Jankowska, B Skupien-Ra, R Iwanicka-N, H Kossowska, A Fogtman, B Rybicka, J Bogus?awsk, A Adamiok-Os, K Hanusek, J Hanusek, M Koblowska, AR Fernie, A Piekie?ko-
Cancer Cell International, 2023-01-05;23(1):2.
Species: Human
Sample Types: Cell Culture Supernates
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Macrophage Phenotypes and Gene Expression Patterns Are Unique in Naturally Occurring Metabolically Healthy Obesity
Authors: AD Ruggiero, R Vemuri, M Block, D DeStephani, M Davis, J Chou, A Williams, A Brock, SK Das, K Kavanagh
International Journal of Molecular Sciences, 2022-10-21;23(20):.
Species: Primate - Chlorocebus aethiops (African Green Monkey)
Sample Types: Plasma
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Human iPSC co-culture model to investigate the interaction between microglia and motor neurons
Authors: BF Vahsen, E Gray, A Candalija, KML Cramb, J Scaber, R Dafinca, A Katsikoudi, Y Xu, L Farrimond, R Wade-Marti, WS James, MR Turner, SA Cowley, K Talbot
Scientific Reports, 2022-07-23;12(1):12606.
Species: Human
Sample Types: Cell Culture Supernates
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Evaluation of markers of fibrinolysis and coagulation in pregnant women with human immunodeficiency virus
Authors: E Schapkaitz, E Libhaber, BF Jacobson, M Toman, A Gerber, HR Büller
Thrombosis Research, 2022-07-07;217(0):1-8.
Species: Human
Sample Types: Plasma
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RAGE has potential pathogenetic and prognostic value in nonintubated hospitalized patients with COVID-19
Authors: KD Wick, L Siegel, JD Neaton, C Oldmixon, J Lundgren, RL Dewar, HC Lane, BT Thompson, MA Matthay, ACTIV-3/TI
JCI Insight, 2022-05-09;7(9):.
Species: Human
Sample Types: Cell Culture Supernates
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PAI-1 is a vascular cell-specific HIF-2-dependent angiogenic factor that promotes retinal neovascularization in diabetic patients
Authors: Y Qin, J Zhang, S Babapoor-F, B Applewhite, M Deshpande, H Megarity, M Flores-Bel, S Aparicio-D, T Ma, Y Rui, SY Tzeng, JJ Green, MV Canto-Sole, S Montaner, A Sodhi
Science Advances, 2022-03-02;8(9):eabm1896.
Species: Human
Sample Types: Cell Culture Supernates
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Glomerular endothelial cell senescence drives age-related kidney disease through PAI-1
Authors: C Cohen, O Le Goff, F Soysouvanh, F Vasseur, M Tanou, C Nguyen, L Amrouche, J Le Guen, O Saltel-Ful, T Meunier, T Nguyen-Kho, M Rabant, D Nochy, C Legendre, G Friedlande, BG Childs, DJ Baker, B Knebelmann, D Anglicheau, F Milliat, F Terzi
Embo Molecular Medicine, 2021-11-02;13(11):e14146.
Species: Human
Sample Types: Urine
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The Activin/FLRG pathway associates with poor COVID-19 outcomes in hospitalized patients
Authors: M McAleavy, Q Zhang, PJ Ehmann, J Xu, MF Wipperman, D Ajithdoss, L Pan, M Wakai, R Simonson, A Gadi, A Oyejide, SC Hamon, A Boyapati, LG Morton, T Shavlakadz, CA Kyratsous, DJ Glass
Molecular and Cellular Biology, 2021-11-01;0(0):MCB0046721.
Species: Human
Sample Types: Cell Culture Supernates
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Genetic Polymorphisms in miR-604A>G, miR-938G>A, miR-1302-3C>T and the Risk of Idiopathic Recurrent Pregnancy Loss
Authors: SH Cho, JH Kim, HJ An, YR Kim, EH Ahn, JR Lee, JO Kim, JJ Ko, NK Kim
International Journal of Molecular Sciences, 2021-06-07;22(11):.
Species: Human
Sample Types: Plasma
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Effects of antithrombotic drugs on the prothrombotic state in patients with atrial fibrillation: The west Birmingham atrial fibrillation project
Authors: C Voukalis, GYH Lip, E Shantsila
Thrombosis Research, 2021-02-09;200(0):149-155.
Species: Human
Sample Types: Plasma
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Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages
Authors: YY Wang, HD Chen, S Lo, YK Chen, YC Huang, SC Hu, YC Hsieh, AC Hung, MF Hou, SF Yuan
Cancers, 2020-11-26;12(12):.
Species: Human
Sample Types: Cell Culture Supernates
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Expression of trophoblast derived prostaglandin E2 receptor 2 (EP2) is reduced in patients with recurrent miscarriage and EP2 regulates cell proliferation and expression of inflammatory cytokines
Authors: L Peng, Y Ye, H Mullikin, L Lin, C Kuhn, M Rahmeh, S Mahner, U Jeschke, V von Schönf
J Reprod Immunol, 2020-10-01;142(0):103210.
Species: Human
Sample Types: Cell Culture Supernates
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GPCR-induced YAP activation sensitizes fibroblasts to profibrotic activity of TGFbeta1
Authors: K Zmajkovico, Y Bauer, K Menyhart, M Schnoebele, D Freti, M Boucher, B Renault, R Studer, M Birker-Rob, A Klenk, O Nayler, J Gatfield
PLoS ONE, 2020-02-13;15(2):e0228195.
Species: Human
Sample Types: Cell Culture Supernates
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Longitudinal Changes of PAI-1, MMP-2, and VEGF in Peritoneal Effluents and Their Associations with Peritoneal Small-Solute Transfer Rate in New Peritoneal Dialysis Patients
Authors: N Hao, TT Chiou, CH Wu, YY Lei, PL Liang, MC Chao, H Yang, JB Chen
Biomed Res Int, 2019-05-05;2019(0):2152584.
Species: Human
Sample Types: Peritoneal Fluid
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Anti ?-enolase antibody is a novel autoimmune biomarker for unexplained recurrent miscarriages
Authors: Y Ye, C Kuhn, M Kösters, GJ Arnold, H Ishikawa-A, C Schulz, N Rogenhofer, CJ Thaler, S Mahner, T Fröhlich, U Jeschke, V von Schönf
EBioMedicine, 2019-03-01;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Senolytics in idiopathic pulmonary fibrosis: Results from a first-in-human, open-label, pilot study
Authors: JN Justice, AM Nambiar, T Tchkonia, NK LeBrasseur, R Pascual, SK Hashmi, L Prata, MM Masternak, SB Kritchevsk, N Musi, JL Kirkland
EBioMedicine, 2019-01-05;0(0):.
Species: Human
Sample Types: Serum
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Prostaglandin E2 receptor 3 (EP3) signaling is induced in placentas with unexplained recurrent pregnancy losses (uRPL)
Authors: Y Ye, A Vattai, N Ditsch, C Kuhn, M Rahmeh, S Mahner, M Ripphahn, R Immler, M Sperandio, U Jeschke, V von Schoen
Endocr Connect, 2018-04-26;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Platelet Carbonic Anhydrase II, a Forgotten Enzyme, May Be Responsible for Aspirin Resistance
Authors: M Jakubowski, J D?bski, E Szahidewic, A Turek-Jaku, J Gawry?, K Gawry?, R Skomro, A Derkacz, A Doroszko
Oxid Med Cell Longev, 2017-09-27;2017(0):3132063.
Species: Human
Sample Types: Plasma
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Combined serum levels of multiple proteins in tPA-BDNF pathway may aid the diagnosis of five mental disorders
Authors: S Chen, H Jiang, Y Liu, Z Hou, Y Yue, Y Zhang, F Zhao, Z Xu, Y Li, X Mou, L Li, T Wang, J Zhao, C Han, Y Sui, M Wang, Z Yang, Y Lu, Y Zhu, J Li, X Shen, F Sun, Q Chen, H Chen, Y Yuan
Sci Rep, 2017-07-31;7(1):6871.
Species: Human
Sample Types: Serum
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Plasminogen Activator Inhibitor-1 in depression: Results from Animal and Clinical Studies
Sci Rep, 2016-07-26;6(0):30464.
Species: Human
Sample Types: Serum
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MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC.
Authors: Rolandsson Enes S, Andersson Sjoland A, Skog I, Hansson L, Larsson H, Le Blanc K, Eriksson L, Bjermer L, Scheding S, Westergren-Thorsson G
Sci Rep, 2016-07-06;6(0):29160.
Species: Human
Sample Types: Cell Culture Supernates
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MAP3K19 Is a Novel Regulator of TGF-? Signaling That Impacts Bleomycin-Induced Lung Injury and Pulmonary Fibrosis
PLoS ONE, 2016-05-04;11(5):e0154874.
Species: Human
Sample Types: Cell Lysates
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Circadian misalignment increases cardiovascular disease risk factors in humans
Proc. Natl. Acad. Sci. U.S.A., 2016-02-08;113(10):E1402-11.
Species: Human
Sample Types: Plasma
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p-Cresol affects reactive oxygen species generation, cell cycle arrest, cytotoxicity and inflammation/atherosclerosis-related modulators production in endothelial cells and mononuclear cells.
Authors: Chang M, Chang H, Chan C, Yeung S, Hsien H, Lin B, Yeh C, Tseng W, Tseng S, Jeng J
PLoS ONE, 2014-12-17;9(12):e114446.
Species: Human
Sample Types: Whole Cells
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Coinfection with human herpesvirus 8 is associated with persistent inflammation and immune activation in virologically suppressed HIV-infected patients.
Authors: Masia M, Robledano C, Ortiz de la Tabla V, Antequera P, Lumbreras B, Hernandez I, Gutierrez F
PLoS ONE, 2014-08-18;9(8):e105442.
Species: Human
Sample Types: Plasma
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Vitamin D insufficiency and subclinical atherosclerosis in non-diabetic males living with HIV.
Authors: Portilla J, Moreno-Perez O, Serna-Candel C, Escoin C, Alfayate R, Reus S, Merino E, Boix V, Giner L, Sanchez-Paya J, Pico A
J Int AIDS Soc, 2014-05-13;17(0):18945.
Species: Human
Sample Types: Serum
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Characterization of circulating and endothelial progenitor cells in patients with extreme-duration type 1 diabetes.
Authors: Hernandez S, Gong J, Chen L, Wu I, Sun J, Keenan H, King G
Diabetes Care, 2014-04-29;37(8):2193-201.
Species: Human
Sample Types: Plasma
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Ghrelin protects against renal damages induced by angiotensin-II via an antioxidative stress mechanism in mice.
Authors: Fujimura K, Wakino S, Minakuchi H, Hasegawa K, Hosoya K, Komatsu M, Kaneko Y, Shinozuka K, Washida N, Kanda T, Tokuyama H, Hayashi K, Itoh H
PLoS ONE, 2014-04-18;9(4):e94373.
Species: Human
Sample Types: Cell Culture Supernates
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Staphylococcal nuclease domain containing-1 (SND1) promotes migration and invasion via angiotensin II type 1 receptor (AT1R) and TGFbeta signaling.
Authors: Santhekadur P, Akiel M, Emdad L, Gredler R, Srivastava J, Rajasekaran D, Robertson C, Mukhopadhyay N, Fisher P, Sarkar D
FEBS Open Bio, 2014-04-01;4(0):353-61.
Species: Human
Sample Types: Cell Culture Supernates
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Increased metabolite levels of glycolysis and pentose phosphate pathway in rabbit atherosclerotic arteries and hypoxic macrophage.
Authors: Yamashita A, Zhao Y, Matsuura Y, Yamasaki K, Moriguchi-Goto S, Sugita C, Iwakiri T, Okuyama N, Koshimoto C, Kawai K, Tamaki N, Zhao S, Kuge Y, Asada Y
PLoS ONE, 2014-01-23;9(1):e86426.
Species: Human
Sample Types: Cell Culture Supernates
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Dipyridamole decreases inflammatory metalloproteinase-9 expression and release by human monocytes.
Thromb Haemost, 2012-12-13;109(2):280-9.
Species: Human
Sample Types: Cell Culture Supernates
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Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis.
BMC Infect Dis, 2012-06-25;12(0):144.
Species: Human
Sample Types: Plasma
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Recombinant GPI-anchored TIMP-1 stimulates growth and migration of peritoneal mesothelial cells.
Authors: Djafarzadeh R, Sauter M, Notohamiprodjo S, Noessner E, Goyal P, Siess W, Wornle M, Ribeiro A, Himmelein S, Sitter T, Nelson PJ
PLoS ONE, 2012-04-27;7(4):e33963.
Species: Human
Sample Types: Cell Lysates
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Exercise training improves sleep pattern and metabolic profile in elderly people in a time-dependent manner.
Authors: Lira FS, Pimentel GD, Santos RV, Oyama LM, Damaso AR, Oller do Nascimento CM, Viana VA, Boscolo RA, Grassmann V, Santana MG, Esteves AM, Tufik S, de Mello MT
Lipids Health Dis, 2011-07-06;10(0):113.
Species: Human
Sample Types: Plasma
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Endothelial differentiation of adipose-derived stem cells from elderly patients with cardiovascular disease.
Authors: Zhang P, Moudgill N, Hager E, Tarola N, Dimatteo C, McIlhenny S, Tulenko T, DiMuzio PJ
Stem Cells Dev., 2010-11-01;20(6):977-88.
Species: Human
Sample Types: Cell Culture Supernates
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Cyclic adenosine monophosphate induces plasminogen activator inhibitor-1 expression in human mast cells.
Authors: Ma Z, Kwong KY, Tovar JP, Paek D
Biochem. Biophys. Res. Commun., 2010-09-08;400(4):569-74.
Species: Human
Sample Types: Cell Culture Supernates
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Placenta growth factor (PlGF), a novel inducer of plasminogen activator inhibitor-1 (PAI-1) in sickle cell disease (SCD).
Authors: Patel N, Sundaram N, Yang M
J. Biol. Chem., 2010-03-29;285(22):16713-22.
Species: Human
Sample Types: Plasma
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Human CD4- 8- T cells are a distinctive immunoregulatory subset.
Authors: Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ
FASEB J, 2010-02-12;24(7):2558-66.
Species: Human
Sample Types: Cell Culture Supernates
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SPARC suppresses apoptosis of idiopathic pulmonary fibrosis fibroblasts through constitutive activation of beta-catenin.
Authors: Chang W, Wei K, Jacobs SS, Upadhyay D, Weill D, Rosen GD
J. Biol. Chem., 2010-01-08;285(11):8196-206.
Species: Human
Sample Types: Cell Culture Supernates
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Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway.
Authors: Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R
J. Pathol., 2009-05-01;218(1):131-42.
Species: Human
Sample Types: Cell Culture Supernates
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Urokinase induces survival or pro-apoptotic signals in human mesangial cells depending on the apoptotic stimulus.
Authors: Tkachuk N, Kiyan J, Tkachuk S, Kiyan R, Shushakova N, Haller H, Dumler I
Biochem. J., 2008-10-15;415(2):265-73.
Species: Human
Sample Types: Cell Culture Supernates
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Adiponectin regulates albuminuria and podocyte function in mice.
Authors: Sharma K, Ramachandrarao S, Qiu G, Usui HK, Zhu Y, Dunn SR, Ouedraogo R, Hough K, McCue P, Chan L, Falkner B, Goldstein BJ
J. Clin. Invest., 2008-05-01;118(5):1645-56.
Species: Human
Sample Types: Plasma
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