Human RIPK3/RIP3 Antibody Summary
Met1-Arg218
Accession # Q9Y572
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human RIPK3/RIP3 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB7604) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RIPK3/RIP3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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RIPK3/RIP3 in Human Spleen. RIPK3/RIP3 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB7604) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RIPK3/RIP3
RIP3 (Receptor-Interacting Protein 3), also known as RIPK3, is a 518 amino acid (aa), 60 kDa phosphoprotein that contains an N-terminal protein kinase domain and a C-terminal interaction motif with which it binds RIP1. It converts cell response to TNF-a from apoptosis (RIP1 via Fas) to programmed necrosis (RIP1/RIP3) by inducing reactive oxygen species production. Human RIP3 (aa 1-218) shares approximately 74% aa sequence identity with mouse and rat RIP3. Alternately spliced human b (252 aa) and g (231 aa) isoforms diverge after aa 219 and 221, respectively, and are found to antagonize RIP3.
Product Datasheets
Citations for Human RIPK3/RIP3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Necroptosis contributes to the intestinal toxicity of deoxynivalenol and is mediated by methyltransferase SETDB1
Authors: Zhou, B;Xiao, K;Guo, J;Xu, Q;Xu, Q;Lv, Q;Zhu, H;Zhao, J;Liu, Y;
Journal of hazardous materials
Species: Porcine
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot -
Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis
Authors: YA Kung, HJ Chiang, ML Li, YN Gong, HP Chiu, CT Hung, PN Huang, SY Huang, PY Wang, TA Hsu, G Brewer, SR Shih
MBio, 2022-01-18;0(0):e0271721.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
ABT?737, a Bcl?2 family inhibitor, has a synergistic effect with apoptosis by inducing urothelial carcinoma cell necroptosis
Authors: R Cheng, X Liu, Z Wang, K Tang
Molecular Medicine Reports, 2021-03-31;23(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Co-Immunoprecipitation, Western Blot -
Prognostic Significance of CHIP and RIPK3 in Non-Small Cell Lung Cancer
Authors: J Kim, JY Chung, YS Park, SJ Jang, HR Kim, CM Choi, JS Song
Cancers (Basel), 2020-06-08;12(6):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
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