Human Proteinase 3/Myeloblastin/PRTN3 Antibody
Human Proteinase 3/Myeloblastin/PRTN3 Antibody Summary
Ala26-Arg249
Accession # P24158
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Proteinase 3/Myeloblastin/ PRTN3 by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, human peripheral blood lymphocytes (PBL), and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Proteinase 3/Myeloblastin/PRTN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6134) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Proteinase 3/Myeloblastin/ PRTN3 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Detection of Human Proteinase 3/Myeloblastin/PRTN3 by Simple WesternTM. Simple Western lane view shows lysates of human peripheral blood mononuclear cell tissue, loaded at 0.2 mg/mL. A specific band was detected for Proteinase 3/Myeloblastin/PRTN3 at approximately 32 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Proteinase 3/Myeloblastin/PRTN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6134) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Proteinase 3/Myeloblastin/PRTN3
Proteinase 3 (PRTN3), also known as Myeloblastin (MBN), is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human PRTN3 proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221 aa mature enzyme (aa 28-248), and an eight aa C‑terminal propeptide (aa 249-256). Within the cell, a 35 kDa immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa 26-249, human PRTN3 shares 68% aa identity with mouse PRTN3.
Product Datasheets
Citation for Human Proteinase 3/Myeloblastin/PRTN3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Dysregulation of Autoantigen Genes in ANCA-Associated Vasculitis Involves Alternative Transcripts and New Protein Synthesis
Authors: Elizabeth A. McInnis, Anshul K. Badhwar, Akhil Muthigi, Olivier M. Lardinois, S. Colby Allred, Jiajin Yang et al.
Journal of the American Society of Nephrology
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