Human Proteinase 3/Myeloblastin/PRTN3 Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Proteinase 3/Myeloblastin/PRTN3

Leukocyte proteinase 3 (PRTN3 or PR3), also called Myeloblastin (MBN) or NP-4, is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human MBN proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221 aa mature enzyme (aa 28-248), and an eight aa C-terminal propeptide (aa 249-256). Within the cell, a 35 kDa immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa 26-249, human MBN shares 68% aa sequence identity with mouse MBN.

Product Datasheets

Related Research Areas

• Airway Inflammation and Remodeling
• Inflammatory Mediators
• Neutrophils
• Serine Proteases and Regulators