Human Proprotein Convertase 9/PCSK9 Antibody Summary
Gln31-Gln692
Accession # Q8NBP7
Applications
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human Proprotein Convertase 9/PCSK9 Monoclonal Antibody (Catalog # MAB38883).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Proprotein Convertase 9/PCSK9 DuoSet ELISA Kit (Catalog # DY3888) for convenient development of a sandwich ELISA or the Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit (Catalog # DPC900) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Proprotein Convertase 9/PCSK9 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3888) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pre-Proprotein Convertase 9/PCSK9 at approximately 80 kDa and mature Proprotein Convertase 9/PCSK9 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Human Proprotein Convertase 9/PCSK9 ELISA Standard Curve. Recombinant Human Proprotein Convertase 9/PCSK9 protein was serially diluted 2-fold and captured by Rat Anti-Human Proprotein Convertase 9/PCSK9 Monoclonal Antibody (Catalog # MAB38883) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3888) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Proprotein Convertase 9/PCSK9
The human PCSK9 gene encodes Proprotein Convertase 9 (PC9), which is also known as Neural Apoptosis Regulated Convertase 1 (NARC1) (1). The deduced amino acid sequence of human PCSK9 consists of a signal peptide (residues 1-30), a pro peptide (residue 31-152), and a mature chain (residues 153-692) that contains a serine protease domain (residues 161-431) found in members of the furin/PC family. PCSK9 protease activity may be limited, since it has only been demonstrated through its own autocatalytic processing (2). After the autocleavage in the ER, the pro domain and mature chain exit the cell together through non-covalent interactions (3). PCSK9 is a key regulator of LDL-cholesterol levels (LDL-C) through binding of the LDL receptor, resulting in the reduction of receptor recycling to the cell surface and the acceleration of receptor degradation in lysosomes (3). Both gain of function (GOF) and loss-of-function (LOF) mutations have been found in the PCSK9 gene (3). GOF mutations are linked to familial autosomal dominant hypercholesterolemia, a disease characterized by elevated plasma levels of LDL-C. In comparison, LOF mutations lead to low levels of LDL-C and protection against coronary heart disease.
- Seidah, N.G. et al. (2003) Proc. Natl. Acad. Sci. USA 100:928.
- Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55.
- Costet, P. et al. (2008) Trends Biochem. Sci. 33:426.
Product Datasheets
Citations for Human Proprotein Convertase 9/PCSK9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Maximizing in vivo target clearance by design of pH-dependent target binding antibodies with altered affinity to FcRn
Authors: Yang D, Giragossian C, Castellano S et al.
MAbs
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PCSK9 is not secreted from mature differentiated intestinal cells
Authors: François Moreau, Aurélie Thédrez, Damien Garçon, Audrey Ayer, Thibaud Sotin, Wieneke Dijk et al.
Journal of Lipid Research
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Identification of 4-Aminopyrazolopyrimidine Metabolite That May Contribute to the Hypolipidemic Effects of LY2584702 in Long Evans Diet–Induced Obese Rats
Authors: Thomas B. Estridge, Asim B. Dey, Charles Reidy, Xiaohong Yu, Yuke Zhang, Maryalice Hartley et al.
Journal of Pharmacology and Experimental Therapeutics
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Targeting PCSK9 in Liver Cancer Cells Triggers Metabolic Exhaustion and Cell Death by Ferroptosis
Authors: M Alannan, H Fatrouni, V Trézéguet, F Dittrich-D, P Moreau, G Siegfried, B Liet, AM Khatib, CF Grosset, B Badran, H Fayyad-Kaz, AJ Merched
Cells, 2022-12-23;12(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Rewiring Lipid Metabolism by Targeting PCSK9 and HMGCR to Treat Liver Cancer
Authors: M Alannan, V Trézéguet, ND Amoêdo, R Rossignol, W Mahfouf, HR Rezvani, F Dittrich-D, P Moreau, S Lacomme, E Gontier, CF Grosset, B Badran, H Fayyad-Kaz, AJ Merched
Cancers, 2022-12-20;15(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Proprotein convertase PCSK9 affects expression of key surface proteins in human pancreatic beta cells via intra- and extracellular regulatory circuits
Authors: K Saitoski, M Ryaboshapk, GM Hamza, AF Jarnuczak, C Berthault, F Carlotti, M Armanet, K Sengupta, CR Underwood, S Andersson, I Guillas, W Le Goff, R Scharfmann
Oncogene, 2022-06-02;0(0):102096.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells
Authors: X Li, J Guo, N Liang, X Jiang, Y Song, S Ou, Y Hu, R Jiao, W Bai
Front Pharmacol, 2018-02-27;9(0):159.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Heparan sulfate proteoglycans present PCSK9 to the LDL receptor
Authors: C Gustafsen, D Olsen, J Vilstrup, S Lund, A Reinhardt, N Wellner, T Larsen, CBF Andersen, K Weyer, JP Li, PH Seeberger, S Thirup, P Madsen, S Glerup
Nat Commun, 2017-09-11;8(1):503.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Common Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Epitopes Mediate Multiple Routes for Internalization and Function.
Authors: DeVay R, Yamamoto L, Shelton D, Liang H
PLoS ONE, 2015-04-23;10(4):e0125127.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Loss of plasma proprotein convertase subtilisin/kexin 9 (PCSK9) after lipoprotein apheresis.
Authors: Tavori H, Giunzioni I, Linton M, Fazio S
Circ Res, 2013-10-11;113(12):1290-5.
Species: Human
Sample Types: Plasma
Applications: Western Blot -
Antibody-mediated disruption of the interaction between PCSK9 and the low-density lipoprotein receptor.
Authors: Duff CJ, Scott MJ, Kirby IT, Hutchinson SE, Martin SL, Hooper NM
Biochem. J., 2009-05-01;419(3):577-84.
Species: Human
Sample Types: Recombinant Protein
Applications: Neutralization
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