Home / GDF-15 / Human/Primate GDF-15 Antibody

Human/Primate GDF-15 Antibody

Catalog #: MAB957
16 Citations 7 Reviews Datasheet
Catalog # Availability Size / Price Qty
MAB957-SP
MAB957-100
MAB957-500
Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (7)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate GDF-15 Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human GDF-15 in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human GDF‑11, recombinant mouse (rm) GDF‑5, rmGDF‑6, rmGDF‑7, or rmGDF‑8 is observed.
Source
Monoclonal Mouse IgG2B Clone # 147627
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human GDF-15
Ala197-Ile308
Accession # Q99988
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human GDF‑15 (Catalog # 957-GD)
under non-reducing conditions only

Human/Primate GDF-15 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate GDF‑15 Biotinylated Antibody (Catalog # BAF940)

Standard: Recombinant Human GDF-15 (CHO-expressed) Protein (Catalog # 957-GD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-15

Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), placental transforming growth factor-beta, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-beta ) superfamily. GDF-15 is highly expressed in placenta and is expressed at lower levels in kidney, pancreas, prostate and colon. It is also widely expressed in brain. Similarly to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of GDF-15 contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Furthermore, the carboxy-terminal domain contains two additional cysteine residues that form a fourth intrachain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the carboxy-terminal 112 amino acid residues. Mature human GDF-15 shares 66.1% and 68.7% amino acid sequence similarity with rat and mouse GDF-15, respectively, which are remarkably low homologies between species in TGF-beta superfamily. GDF-15 has been shown to have various functions, including inhibition of production of tumor necrosis factor alpha (TNF-alpha ) from lipopolysaccharide-stimulated macrophages, induction of cartilage formation, early-stage endochonadal bone formation, and promotion of neuronal survival.

References
  1. Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514.
  2. Böttner, M. et al. (1999) Gene 237:105.
  3. Fairlie, W.D. et al. (1998) J. Leukoc. Biol 65:2.
  4. Fairlie, W.D. et al. (2001) J B.C 20:16911.
  5. Bauskin, A.R. et al. (2000) EMBO J. 19:2212.
  6. Strelau, J. et al. (2000) J. Neurosci. 20:8597.
  7. Schober, A. et al. (2001) J. Comp. Neurol. 439:32.
Long Name
Growth Differentiation Factor 15
Entrez Gene IDs
9518 (Human); 23886 (Mouse); 29455 (Rat)
Alternate Names
GDF15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC-1NSAID-activated gene 1 protein; MIC1Prostate differentiation factor; NAG-1; NAG-1NSAID-regulated gene 1 protein; NSAID (nonsteroidal inflammatory drug)-activated protein 1; PDF; PDFGDF-15; PLAB; PLABNRG-1; Placental bone morphogenetic protein; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta

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Citations for Human/Primate GDF-15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. GDF15 promotes osteosarcoma cell migration and invasion by regulating the TGF‑ beta signaling pathway
    Authors: Guangfu Chen, Min Wang, Xiang Liu
    Molecular Medicine Reports
  2. Growth differentiation factor 15 is a promising diagnostic and prognostic biomarker in colorectal cancer
    Authors: Chen Li, Xiaobing Wang, Ignacio Casal, Jingyu Wang, Peiwei Li, Wei Zhang et al.
    Journal of Cellular and Molecular Medicine
  3. Growth differentiation factor 15 induces cisplatin resistance through upregulation of xCT expression and glutathione synthesis in gastric cancer
    Authors: Wang, SF;Chang, YL;Fang, WL;Li, AF;Chen, CF;Yeh, TS;Hung, GY;Huang, KH;Lee, HC;
    Cancer science
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Western Blot, Neutralization
  4. Increased expression and accumulation of GDF15 in IPF extracellular matrix contribute to fibrosis
    Authors: A Radwanska, CT Cottage, A Piras, C Overed-Say, C Sihlbom, R Budida, C Wrench, J Connor, S Monkley, P Hazon, H Schluter, MJ Thomas, CM Hogaboam, LA Murray
    JCI Insight, 2022-08-22;7(16):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Circulating levels of GDF15 in patients with myalgic encephalomyelitis/chronic fatigue syndrome
    Authors: A Melvin, E Lacerda, HM Dockrell, S O'Rahilly, L Nacul
    J Transl Med, 2019-12-04;17(1):409.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  6. TGF-? contamination of purified recombinant GDF15
    Authors: OE Olsen, A Skjærvik, BF Størdal, A Sundan, T Holien
    PLoS ONE, 2017-11-21;12(11):e0187349.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. GDF15/MIC1 and MMP9 Cerebrospinal Fluid Levels in Parkinson's Disease and Lewy Body Dementia.
    Authors: Walter Maetzler, Willy Deleersni, Valérie Hanssens, Alice Bernard, Kathrin Brockmann, Justus Marquetan, Isabel Wurster, Tim W Rattay, Lorenzo Roncoroni, Eva Schaeffer, Stefanie Lerche, Anja Apel, Christian Deuschle, Daniela Berg
    PLoS ONE, 2016-03-03;0(0):1932-6203.
    Species: Human
    Sample Types: Beads
    Applications: Luminex Development
  8. Macrophage inhibitory cytokine 1 biomarker serum immunoassay in combination with PSA is a more specific diagnostic tool for detection of prostate cancer.
    Authors: Li, Ji, Veltri, Robert W, Yuan, Zhen, Christudass, Christhu, Mandecki, Wlodek
    PLoS ONE, 2015-04-08;10(4):e0122249.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  9. Growth differentiating factor 15 enhances the tumor-initiating and self-renewal potential of multiple myeloma cells.
    Authors: Tanno, Toshihik, Lim, Yiting, Wang, Qiuju, Chesi, Marta, Bergsagel, P Leif, Matthews, Geoff, Johnstone, Ricky W, Ghosh, Nilanjan, Borrello, Ivan, Huff, Carol An, Matsui, William
    Blood, 2013-12-17;123(5):725-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Growth differentiation factor-15 predicts mortality and morbidity after cardiac resynchronization therapy.
    Authors: Foley PW, Stegemann B, Ng K, Ramachandran S, Proudler A, Frenneaux MP, Ng LL, Leyva F
    Eur. Heart J., 2009-08-07;30(22):2749-57.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  11. Growth differentiation factor-15 as a prognostic marker in patients with acute myocardial infarction.
    Authors: Khan SQ, Ng K, Dhillon O, Kelly D, Quinn P, Squire IB, Davies JE, Ng LL
    Eur. Heart J., 2009-01-23;30(9):1057-65.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  12. Diet-induced macrophage inhibitory cytokine 1 promotes prostate cancer progression
    Authors: Mingguo Huang, Shintaro Narita, Takamitsu Inoue, Norihiko Tsuchiya, Shigeru Satoh, Hiroshi Nanjo et al.
    Endocrine-Related Cancer
  13. Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer
    Authors: Jyun-Yuan Huang, Yen-Yun Wang, Steven Lo, Ling-Ming Tseng, Dar-Ren Chen, Yi-Chia Wu et al.
    Cancers (Basel)
  14. Hyperbaric Oxygen Exposure Attenuates Circulating Stress Biomarkers: A Pilot Interventional Study
    Authors: Jae Seung Chang, Eunha Chang, Yoonsuk Lee, Yong Sung Cha, Seung-Kuy Cha, Won Gil Cho et al.
    International Journal of Environmental Research and Public Health
  15. The role of growth differentiation factor 15 in the pathogenesis of primary myelofibrosis.
    Authors: Uchiyama T, Kawabata H, Miura Y et al.
    Cancer Med
  16. GDF15 promotes EMT and metastasis in colorectal cancer
    Authors: Chen Li, Jingyu Wang, Jianlu Kong, Jinlong Tang, Yihua Wu, Enping Xu et al.
    Oncotarget

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Reviews for Human/Primate GDF-15 Antibody

Average Rating: 4.6 (Based on 7 Reviews)

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Human/Primate GDF-15 Antibody
By Anonymous on 12/26/2021
Application: WB Sample Tested: EDTA Plasma Species: Human
Western Blot Human/Primate GDF-15 Antibody MAB957
Human/Primate GDF-15 Antibody
By Anonymous on 08/31/2021
Application: WB Sample Tested: Serum Species: Human
Western Blot Human/Primate GDF-15 Antibody MAB957
Human/Primate GDF-15 Antibody
By Anonymous on 07/08/2020
Application: ELISA Sample Tested: Recombinant protein Species: Human
ELISA Human/Primate GDF-15 Antibody MAB957
Human/Primate GDF-15 Antibody
By Anonymous on 11/08/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human
ELISA Human/Primate GDF-15 Antibody MAB957
Human/Primate GDF-15 Antibody
By Anonymous on 06/11/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human
ELISA Human/Primate GDF-15 Antibody MAB957
Human/Primate GDF-15 Antibody
By Balaji Mahender on 12/20/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human
Human/Primate GDF-15 Antibody
By Ruhul Choudhury on 01/22/2017
Application: B/N Sample Tested: Extravillous trophoblasts conditioned medium Species: Mouse